中国组织工程研究

中国组织工程研究 ›› 2013, Vol. 17 ›› Issue (37): 6607-6612.doi: 10.3969/j.issn.2095-4344.2013.37.011

• 组织构建细胞学实验 cytology experiments in tissue construction • 上一篇    下一篇

基因芯片分析鱼藤素对斑马鱼胚胎发育的影响

李文浩1, 2，刘瑞瑾2，吴新荣1, 2   

  1. 1解放军广州军区广州总医院药剂科，广东省广州市  510010
    2华南理工大学轻工与食品学院，广东省广州市  510641
  • 收稿日期:2012-07-27 修回日期:2012-10-13 出版日期:2013-09-10 发布日期:2013-09-10
  • 通讯作者: 吴新荣，主任药师，博士生导师，华南理工大学轻工与食品学院，广东省广州市 510641 gzwxrong@yahoo.com.cn
  • 作者简介:李文浩★，男，1986年生，广东省五华县人，华南理工大学在读硕士，主要从事抗肿瘤药物方面的研究。 lwh288@qq.com
  • 基金资助:

    广东省自然科学基金(10251001002000000)*，课题名称：基于flk-GFP 斑马鱼模型的鱼藤素血管生成抑制作用机制研究

Deguelin treatment delays embryonic development of zebrafish: A gene chip analysis

Li Wen-hao1,2, Liu Rui-jin2, Wu Xin-rong1, 2   

  1. 1Department of Pharmacy, General Hospital of Guangzhou Military Command of PLA, Guangzhou  510010, Guangdong Province, China
    2School of Light Industry and Food, South China University of Technology, Guangzhou  510641, Guangdong Province, China
  • Received:2012-07-27 Revised:2012-10-13 Online:2013-09-10 Published:2013-09-10
  • Contact: Wu Xin-rong, Chief pharmacist, Doctoral supervisor, Department of Pharmacy, General Hospital of Guangzhou Military Command of PLA, Guangzhou 510010, Guangdong Province, China; School of Light Industry and Food, South China University of Technology, Guangzhou 510641, Guangdong Province, China gzwxrong@yahoo.com.cngzwxrong@yahoo.com.cn
  • About author:Li Wen-hao★, Studying for master’s degree, School of Light Industry and Food, South China University of Technology, Guangzhou 510641, Guangdong Province, China lwh288@qq.com
  • Supported by:

    the Natural Science Foundation of Guangdong Province, No. 10251001002000000*

PDF

532

被引次数

2

摘要：

背景：鱼藤素具有延缓斑马鱼胚胎发育的作用，但其具体作用机制尚未明确。
目的：分析鱼藤素处理斑马鱼胚胎的基因与信号传导通路表达差异。
方法：斑马鱼胚胎来自于根据Zebrafish book中描述的方法养的殖斑马鱼。用0.6 μmol/L鱼藤素处理2-细胞期斑马鱼胚胎，以二甲基亚砜孵化液处理2-细胞期斑马鱼胚胎为对照。4 hpf 时期提取二甲基亚砜组及鱼藤素组总RNA，利用基因芯片技术检测鱼藤素处理斑马鱼胚胎后基因的差异表达，采用实时荧光定量PCR验证基因芯片结果，结合聚类分析及通路分析来探索鱼藤素的作用机制。
结果与结论：芯片结果显示，鱼藤素处理斑马鱼胚胎后差异表达上调3倍以上的基因407个，下调的461个。PCR 验证结果与芯片结果相符。经KEGG 通路分析，涉及14条通路。鱼藤素可能通过干扰细胞的代谢生长及影响其分化起作用。

关键词: 组织构建, 组织构建细胞学实验, 鱼藤素, 斑马鱼, 抗肿瘤, 胚胎发育, 胚胎, 基因芯片, 信号通路, 差异表达, 基因, 省级基金

Abstract:

BACKGROUND: Anticancer drug deguelin delays the embryonic development of zebrafish, but its exact mechanism is not yet clear.
OBJECTIVE: To explore expression differences in genes and signaling pathways in deguelin-treated zebrafish embryos.
METHODS: Zebrafish embryos were harvested from zebrafish fed according to the Zebrafish Book. Zebrafish embryos at 2-cell stage were selected and divided into two groups: control group treated with dimethyl sulfoxide incubator liquid, and experimental group treated with 0.6 μmol/L deguelin. Total RNA was extracted from the two groups at sphere stage. Then, gene chip technique was used to detect differentially expressed genes in the deguelin-treated zebrafish embryos. Real-time quantitative PCR was employed to validate microarray cluster analysis and pathway analysis to explore the mechanism of action of deguelin.
RESULTS AND CONCLUSION: Chip results showed that 407 genes were upregulated more than three times and 461 genes were downregulated more than three times after deguelin treatment. PCR validation results were consistent with those of the chip. Fourteen pathways were identified by KEGG pathway analysis. Deguelin may play an important role by intervening cell metabolism growth and differentiation.

Key words: derris, zebrafish, embryonic development, signal transduction

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