中国组织工程研究

中国组织工程研究 ›› 2011, Vol. 15 ›› Issue (45): 8404-8407.doi: 10.3969/j.issn.1673-8225.2011.45.010

• 肿瘤干细胞 cancer stem cells • 上一篇    下一篇

MCF7细胞中肿瘤干细胞的增殖特征

黄名威1，陆云飞2   

  1. 1广西医科大学附属肿瘤医院，广西壮族自治区南宁市  530021
    2广西医科大学第一附属医院，广西壮族自治区南宁市 530021
  • 收稿日期:2011-04-14 修回日期:2011-06-01 出版日期:2011-11-05 发布日期:2011-11-05
  • 通讯作者: 陆云飞，硕士，主任医师，广西医科大学第一附属医院，广西壮族自治区南宁市 530021
  • 作者简介:黄名威☆，男，1977年生，广西壮族自治区上林县人，汉族，2010年广西医科大学毕业，博士，主治医师，主要从事肿瘤发生学研究。 hmw503@163.com
  • 基金资助:

    广西研究生教育创新计划资助项目(2008105981002D33)。

Proliferation characteristics of tumor stem cells in MCF7 cell line

Huang Ming-wei1, Lu Yun-fei2   

  1. 1The Affiliated Tumors Hospital of Guangxi Medical University, Nanning  530021, Guangxi Zhuang Autonomous Region, China
    2the First Affiliated Hospital of Guangxi Medical University, Nanning  530021, Guangxi Zhuang Autonomous Region, China
  • Received:2011-04-14 Revised:2011-06-01 Online:2011-11-05 Published:2011-11-05
  • Contact: Lu Yun-fei, Master, Chief physician, the First Affiliated Hospital of Guangxi Medical University, Nanning 530021, Guangxi Zhuang Autonomous Region, China
  • About author:Huang Ming-wei☆, Doctor, Attending physician, the Affiliated Tumors Hospital of Guangxi Medical University, Nanning 530021, Guangxi Zhuang Autonomous Region, China hmw503@163.com
  • Supported by:

    Guangxi Graduate Education Innovation Program, No. 2008105981002D33*

PDF

279

被引次数

2

摘要：

背景：肿瘤干细胞学说认为，肿瘤干细胞是肿瘤不断增殖的根源，并与肿瘤的浸润转移、耐药现象密切相关。
目的：在单细胞水平研究体外传代培养的MCF7细胞中肿瘤干细胞含量变化规律，认识肿瘤干细胞在体外培养环境下的增殖特点。
方法：利用单细胞分离种植-成瘤性克隆方法对传代培养后不同时间点MCF7细胞中肿瘤干细胞的比例连续检测，流式细胞仪同步检测相应细胞样本中CD44+CD24-/low亚群的含量变化。
结果与结论：传代后培养的MCF7细胞在不同时间点其肿瘤干细胞比例及CD44+CD24-/low亚群比例均呈现有规律的变化。但CD44+CD24-/low亚群变化更为显著(36.84%到81.95%)，而肿瘤干细胞含量仅在小范围波动(38.54%~47.39%)。结果提示传代培养的MCF7细胞中，肿瘤干细胞具有通过调节自身增殖来保持其比例相对稳定的特点；CD44+CD24-/low亚群并不代表或者富集MCF7细胞株中的肿瘤干细胞亚群。

关键词: 肿瘤干细胞, 成瘤性克隆, MCF7细胞株, 增殖, CD44+/CD24-/low

Abstract:

BACKGROUND: The hypothesis of tumor stem cells posited that tumor stem cells are the root of tumor proliferation constantly and closely related to tumor biological behaviors such as infiltration, metastasis and drug-resistant.
OBJECTIVE: To investigate the proliferation characteristics of tumor stem cells in MCF7 cell line in vitro by studying the tumorigenic-colony forming rate at single cell level.
METHODS: Tumor stem cells proportion in MCF7 cell line was detected at series timing after passaged culture by single-cell-transplanting and tumorigenic-colony forming assay. The proportion of the CD44+/CD24-/low subset was detected by flow cytometry at the same time.
RESULTS AND CONCLUSION: The proportion of tumor stem cells and the CD44+/CD24-/low subset in MCF7cell line both varied regularly after passaged culture. But the variation of CD44+/CD24-/low (from 36.84% to 81.95%) subset was more drastic than that of tumor stem cells proportion,which only fluctuated in a limited range from 38.54% to 47.39%. The proportion of tumor stem cells was steady in MCF7 cell line after passaged culture, owing to the property of tumor stem cells to modulate and balance the proportion by itself. CD44+/CD24-/low subset neither represented nor enriched tumor stem cells subset in MCF7 cell line.  

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