中国组织工程研究

中国组织工程研究 ›› 2011, Vol. 15 ›› Issue (45): 8408-8412.doi: 10.3969/j.issn.1673-8225.2011.45.011

• 脐带脐血干细胞 umbilical cord blood stem cells • 上一篇    下一篇

人脐带源间充质干细胞体内外移植的免疫学特征

野向阳1，徐  岩1，毕薇薇1，郭立斌1，李相军2，刘建国3   

  1. 1吉林省四平市中心医院骨科，吉林省四平市   136000
    2吉林大学药学院病理教研室，吉林省长春市  130021
    3吉林大学第一医院骨关节科，吉林省长春市  130021
  • 收稿日期:2011-04-20 修回日期:2011-05-31 出版日期:2011-11-05 发布日期:2011-11-05
  • 通讯作者: 刘建国，博士，教授，博士生导师，吉林大学第一医院骨关节科，吉林省长春市 130021 jgliu.2005@yahoo.com.cn
  • 作者简介:野向阳☆，男，1974年生，吉林省四平市人，汉族，博士，副主任医师，主要从事人工关节与关节创伤研究。 xyyegk@yahoo.cn

Immunological characteristics of human umbilical cord derived mesenchymal stem cells transplanted in vivo and vitro

Ye Xiang-yang1, Xu Yan1, Bi Wei-wei1, Guo Li-bin1, Li Xiang-jun2, Liu Jian-guo3   

  1. 1Department of Orthopedics, Central Hospital of Siping City, Siping  136000, Jilin Province, China
    2Department of Pathology, College of Pharmacy, Jilin University, Changchun  130021, Jilin Province, China
    3Department of Bone and Joint, First Hospital of Jilin University, Changchun  130021, Jilin Province, China
  • Received:2011-04-20 Revised:2011-05-31 Online:2011-11-05 Published:2011-11-05
  • Contact: Liu Jian-guo, Doctor, Professor, Doctoral supervisor, Department of Bone and Joint, First Hospital of Jilin University, Changchun 130021, Jilin Province, China jgliu.2005@yahoo.com.cn
  • About author:Ye Xiang-yang☆, Doctor, Associate chief physician, Department of Orthopedics, Central Hospital of Siping City, Siping 136000, Jilin Province, China xyyegk@yahoo.cn

PDF

363

被引次数

5

摘要：

背景：部分体外实验证实人脐带源间充质干细胞具有同种异体的免疫耐受特性，然而对其体内研究尚处于异种异体之间，且结果及机制缺少统一性。
目的：培养人脐带源间充质干细胞，通过体内外试验分析其移植免疫学特性。
方法：应用组织块贴壁法提取人脐带源间充质干细胞，通过体外淋巴细胞混合反应检测其同种异体免疫耐受作用；应用流式细胞仪检测体内行人脐带源间充质干细胞移植前后患者血液中CD4、CD8浓度及比值变化，并通过ELISA方法对比体外淋巴细胞混合培养前后血液、培养上清及体内移植前后患者血液中白细胞介素2，10、γ-干扰素的浓度变化。
结果与结论：体内外实验中，混合淋巴细胞反应前后血浆及细胞上清中的细胞因子浓度变化呈相似趋势：白细胞介素10较反应前上升(P < 0.05)，γ-干扰素较反应前下降(P < 0.05)。人脐带源间充质干细胞移植后患者T细胞亚群中CD4+/CD8+比值较移植前轻度下降。说明人脐带源间充质干细胞具有同种异体免疫耐受及免疫调节作用。

关键词: 人脐带源间充质干细胞, 同种异体, 免疫耐受, 免疫调节, 细胞移植

Abstract:

BACKGROUND: Human umbilical cord derived mesenchymal stem cells (HUC-MSCs) have been confirmed to have the characteristics of immunologic tolerance in vitro, but there are no relevant allogenic research in vivo. The results and mechanism are lack of consistency.
OBJECTIVE: To cultivate HUC-MSCs and research the immunological characteristics of implanted HUC-MSCs in vivo and in vitro.
METHODS: HUC-MSCs were obtained by using tissue adhesion method. Mixed-leukocyte reaction (MLR) was performed to evaluate immunologic tolerance in vitro, flow cytometry applied to detect the changes of CD4 and CD8 expression as well as ratio of CD4 to CD8, and ELISA used to detect interleukin 2 (IL-2), IL-10, γ-interferon (IFN-γ) levels.
RESULTS AND CONCLUSION: MLR results showed a similar trend in the concentration changes of cytokines: the level of IL-10 slightly increased after MLR reaction (P < 0.05), while the level of IFN-γ decreased (P < 0.05). The ratio of CD4+/CD8+ in T cell subsets was decreased to a slight extent after cell transplantation, indicating that HUC-MSCs possessed the characteristics of allogeneic immunological tolerance and immunoloregulation effect.

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