中国组织工程研究

中国组织工程研究 ›› 2011, Vol. 15 ›› Issue (37): 6901-6904.doi: 10.3969/j.issn.1673-8225.2011.37.015

• 组织构建与生物活性因子 tissue construction and bioactive factors • 上一篇    下一篇

香烟提取物影响大鼠主动脉平滑肌细胞结缔组织生长因子的表达

王朋朋，刘闺男，彭  霞   

  1. 中国医科大学附属第一医院心血管内科，辽宁省沈阳市  110001
  • 收稿日期:2011-04-18 修回日期:2011-06-30 出版日期:2011-09-10 发布日期:2011-09-10
  • 通讯作者: 刘闺男，硕士，教授，中国医科大学附属第一医院心血管内科，辽宁省沈阳市 110001 guinanliu@hotmail.com
  • 作者简介:王朋朋★，男，1984年生，山西省晋城市人，汉族，2011年中国医科大学毕业，硕士，主要从事冠状动脉硬化性心脏病的基础及临床研究。 wp10878@163. com
  • 基金资助:

    国家自然科学基金资助项目(30871074)，课题名称：Egr-1与骨桥蛋白相互调控机制及在血管再狭窄中的作用。

Effects of cigarette smoke extract on the expression of connective tissue growth factor in rat aortic smooth muscle cells

Wang Peng-peng, Liu Gui-nan, Peng Xia   

  1. Department of Cardiology, First Affiliated Hospital of China Medical University, Shenyang   110001, Liaoning Province, China
  • Received:2011-04-18 Revised:2011-06-30 Online:2011-09-10 Published:2011-09-10
  • Contact: Liu Gui-nan, Master, Professor, Department of Cardiology, First Affiliated Hospital of China Medical University, Shenyang 110001, Liaoning Province, China guinanliu@hotmail. com
  • About author:Wang Peng-peng★, Master, Department of Cardiology, First Affiliated Hospital of China Medical University, Shenyang 110001, Liaoning Province, China wp10878@163.com
  • Supported by:

    the National Natural Science Foundation of China, No.30871074*

PDF

269

被引次数

4

摘要：

背景：血管平滑肌细胞增殖是心血管系统组织变化的基础。
目的：观察不同浓度香烟提取物对大鼠主动脉平滑肌细胞结缔组织生长因子表达的影响。
方法：取第3代大鼠主动脉平滑肌细胞，分别用体积分数2.5%，5%，10%，20%的香烟提取物进行干预。
结果与结论：实时定量RT-PCR结果显示，体积分数2.5%，5%，10%，20%的香烟提取物作用12 h均可促进大鼠主动脉平滑肌细胞结缔组织生长因子mRNA的表达，以体积分数5%香烟提取物的作用最明显，且其刺激大鼠主动脉平滑肌细胞结缔组织生长因子mRNA的表达在24 h内呈时间依赖性。提示香烟提取物可上调大鼠主动脉平滑肌细胞结缔组织生长因子mRNA的表达，导致大鼠主动脉平滑肌细胞的增殖。

关键词: 香烟提取物, 主动脉平滑肌细胞, 结缔组织生长因子, 增殖, 心血管疾病

Abstract:

BACKGROUND: Vascular smooth muscle cell proliferation is the basis for changes of cardiovascular tissue.
OBJECTIVE: To investigate the effects of different concentrations of cigarette smoke extract on the expression of connective tissue growth factor in rat aortic smooth muscle cells.
METHODS: Passage 3 rat aortic smooth muscle cells were interfered with 2.5%, 5%, 10% and 20% cigarette smoke extract.
RESULTS AND CONCLUSION: Real-time quantitative PCR results showed that treatment with 2.5%, 5%, 10% and 20% cigarette smoke extract for 12 hours can increase mRNA expression of connective tissue growth factor in rat aortic smooth muscle cells, in particular 5% cigarette smoke extract, and that mRNA expression of connective tissue growth factor in rat aortic smooth muscle cells was dose-dependent within 24 hours. Results demonstrated that cigarette smoke extract can upregulate the mRNA expression of connective tissue growth factor in rat aortic smooth muscle cells, leading to the proliferation of rat aortic smooth muscle cells.

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