中国组织工程研究

中国组织工程研究 ›› 2011, Vol. 15 ›› Issue (2): 224-227.doi: 10.3969/j.issn.1673-8225.2011.02.008

• 组织构建实验造模 experimental modeling in tissue construction • 上一篇    下一篇

大鼠种植性CBRH-7919肝癌模型的MRI、数字减影血管造影及病理学表现

沈俊杰，李鹤平，杨建勇，张  冰，侯昌龙   

  1. 中山大学附属第一医院放射介入科，广东省广州市 510080
  • 收稿日期:2010-07-12 修回日期:2010-08-18 出版日期:2011-01-08 发布日期:2011-01-08
  • 通讯作者: 李鹤平，博士，副主任医师，中山大学附属第一医院放射介入科，广东省广州市 510080 jxgdhp@hotmail.com
  • 作者简介:沈俊杰☆，男，1971年生，安徽省巢湖市人，汉族，中山大学在读博士，中级职称，主要从事影像诊断与介入放射学研究。 sjjlsx@126.com
  • 基金资助:

    国家自然科学资金资助(项目批准号：30600156)面上项目，名称：经肝动脉应用IL-12基因转染的免疫活性脾淋巴细胞治疗肝癌的研究。

Magnetic resonance imaging, digital subtraction angiography and pathologic findings of rat implanted CBRH-7919 hepatic cancer models

Shen Jun-jie, Li He-ping,Yang Jian-yong, Zhang Bing, Hou Chang-long   

  1. Department of Interventional Radiology, the First Affiliated Hospital of Sun Yat-sen University, Guangzhou  510080, Guangdong Province, China
  • Received:2010-07-12 Revised:2010-08-18 Online:2011-01-08 Published:2011-01-08
  • Contact: Li He-ping, Doctor, Associate chief physician, Department of Interventional Radiology, the First Affiliated Hospital of Sun Yat-sen University, Guangzhou 510080, Guangdong Province, China jxgdhp@hotmail.com
  • About author:Shen Jun-jie☆, Studying for doctorate, Department of Interventional Radiology, the First Affiliated Hospital of Sun Yat-sen University, Guangzhou 510080, Guangdong Province, China sjjlsx@126.com
  • Supported by:

     the National Natural Science Foundation of China (General Program), No. 30600156

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361

被引次数

3

摘要：

背景：大鼠种植性CBRH-7919肝癌模型是肝癌实验性治疗和影像学诊断研究重要的工具，但模型建立后如何对模型进行检测和验证鲜有报道。
目的：通过彩色超声、MRI及肝动脉血管数字减影血管造影检查的方法来检测和验证Wistar大鼠种植性CBRH-7919肝癌模型，并探讨大鼠种植性CBRH-7919肝癌模型的影像学及病理表现。
方法：CBRH-7919大鼠肝癌细胞瘤株接种裸鼠双侧肩胛部皮下，再将生成的肿瘤组织在开腹直视下移植接种于成年Wistar大鼠肝脏内，建立大鼠种植性CBRH-7919肝癌模型。模型建立4周后用彩超检查、筛选建模成功的Wistar大鼠进行MRI检查、肝动脉血管数字减影血管造影及病理学检查。
结果与结论：在建模成功的35只大鼠中，超声检查发现37个结节，MRI检查发现肝脏内共41个结节，较超声增加4个；最小的结节约3 mm，最大约8 mm。MRI扫描显示T1WI表现为低信号，T2WI及脂肪抑制序列表现高信号。肝动脉血管数字减影血管造影均表现由肝动脉供血的富血供结节。光学显微镜下观察可见肿瘤细胞呈巢状或条块状排列，周围伴行多条血管，肿瘤细胞体积大小不一致，核大而浓染，细胞质少，异型性明显。结果表明彩超和核磁共振扫描是大鼠肝癌检查简单而有效的方法，肝动脉血管数字减影血管造影可以了解肿块的供血动脉及其血供情况。通过对Wistar大鼠种植性肝癌模型的MRI及肝动脉血管数字减影血管造影表现能反映肿瘤的病理形态学和生物学行为改变。

关键词: 数字减影血管造影, Wistar大鼠, 肝肿瘤, 动物模型, MRI, 病理学

Abstract:

BACKGROUND: Rat implanted CBRH-7919 hepatic cancer models are important tools for the experimental therapy and imageologic diagnosis. However, how to determine and verify the established models remains poorly understood.
OBJECTIVE: To study the imageologic and pathologic findings of implanted CBRH-7919 hepatic cancer model in Wistar rats.
METHODS: Concentrated cancerous suspension of CBRH-7919 cell lines were injected subcutaneouly into the nude mice scapular region. Then the tumor tissues were implanted into the liver of adult Wistar rats under direct vision. At 4 weeks after implantation, models were confirmed by color ultrasonograph and observed by imageologic and pathologic examination.
RESULTS AND CONCLUSION: Totally 37 nodes were found by ultrasonograph in 35 successful models, and additional 4 nodes were found by magnetic resonance imaging (MRI), with the diameter of the largest tumor was 8 mm, and the least was 3 mm. The tumor presented with hypointensity signal on T1WI and hyperintensity signal on T2WI and fat-suppression images. Digital subtraction angiography showed that all nodes had a large amount of blood supply which came from the liver arteries. The tumor cells are variable in sizes and shapes, disarranged and remarkable atypia with light microscopic observation. All findings demonstrated that, color ultrasonograph and MRI are effective and convenient to examine the model rats, but digital subtraction angiography can help us to find the blood supply of tumors. MRI and digital subtraction angiography examination can show pathological and biological behavior changes of tumor in rat implanted hepatic cancer models.

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