中国组织工程研究 ›› 2021, Vol. 25 ›› Issue (31): 5025-5033.doi: 10.12307/2021.146

• 干细胞综述 stem cell review • 上一篇    下一篇

间充质干细胞活体示踪:如何实现分布和存活的同时示踪

李  晔1,2,杨育坤1,2,朱向情1,何  洁1,2,王金祥1,王严影1,田  川1,庞荣清1,潘兴华1   

  1. 1解放军联勤保障部队第九二〇医院细胞生物治疗中心,云南省细胞治疗技术转化医学重点实验室,干细胞与免疫细胞生物医药技术国家地方联合工程实验室,云南省昆明市  650032;2昆明医科大学,云南省昆明市  650504
  • 收稿日期:2020-06-15 修回日期:2020-06-19 接受日期:2020-07-31 出版日期:2021-11-08 发布日期:2021-04-25
  • 通讯作者: 潘兴华,博士,主任医师,教授,解放军联勤保障部队第九二〇医院细胞生物治疗中心,云南省细胞治疗技术转化医学重点实验室,干细胞与免疫细胞生物医药技术国家地方联合工程实验室,云南省昆明市 650032
  • 作者简介:李晔,女,1995年生,云南省昆明市人,汉族,昆明医科大学在读博士,主要从事间充质干细胞与再生医学研究。
  • 基金资助:
    云南省重大科技专项计划(2018ZF007),项目负责人:潘兴华;国家自然科学基金项目(31970515),项目负责人:庞荣清

In vivo track technique for mesenchymal stem cells: how to realize simultaneous tracing of distribution and survival

Li Ye1, 2, Yang Yukun1, 2, Zhu Xiangqing1, He Jie1, 2, Wang Jinxiang1, Wang Yanying1, Tian Chuan1, Pang Rongqing1, Pan Xinghua1   

  1. 1Cell Biological Therapy Center of 920th Hospital of Joint Logistics Support Force of Chinese PLA, The Transfer Medicine Key Laboratory of Cell Therapy Technology of Yunnan Province, The Integrated Engineering Laboratory of Cell Biological Medicine of State and Regions, Kunming 650032, Yunnan Province, China; 2Kunming Medical University, Kunming 650504, Yunnan Province, China
  • Received:2020-06-15 Revised:2020-06-19 Accepted:2020-07-31 Online:2021-11-08 Published:2021-04-25
  • Contact: Pan Xinghua, MD, Chief physician, Professor, Cell Biological Therapy Center of 920th Hospital of Joint Logistics Support Force of Chinese PLA, The Transfer Medicine Key Laboratory of Cell Therapy Technology of Yunnan Province, The Integrated Engineering Laboratory of Cell Biological Medicine of State and Regions, Kunming 650032, Yunnan Province, China
  • About author:Li Ye, Doctoral candidate, Cell Biological Therapy Center of 920th Hospital of Joint Logistics Support Force of Chinese PLA, The Transfer Medicine Key Laboratory of Cell Therapy Technology of Yunnan Province, The Integrated Engineering Laboratory of Cell Biological Medicine of State and Regions, Kunming 650032, Yunnan Province, China; Kunming Medical University, Kunming 650504, Yunnan Province, China
  • Supported by:
    the Major Science and Technology Special Plan of Yunnan Province, No. 2018ZF007 (to PXH); the National Natural Science Foundation of China, No. 31970515 (to PRQ)

摘要:

文题释义

活体示踪技术:是一种借助适当的细胞标记方法,通过影像学成像在一定时间内无创地在活体内动态监测移植细胞的迁移、分布、定植和存活情况的一类技术,可为干细胞在体内的转归及其治疗的潜在作用机制提供一些线索,使用适当的一种或多种成像方式可以帮助评估干细胞的疗效和安全性,动态监测干细胞是否出现了恶性转化、畸胎瘤形成等不良事件。
光声成像:是一种借助光声效应将光学成像的对比度和特异性与超声的高时间分辨率结合在一起,机体吸收的激光能量引起组织的快速热弹性膨胀,从而产生超声波,用换能器检测超声波,该换能器将机械声波转换为电信号,所捕获的信号被处理后形成图像的一种成像技术。
背景:干细胞疗法对开发许多疑难疾病的新治疗策略有广阔的应用前景。尽管在过去的几年里,干细胞疗法的临床试验数量有所增加,但由于干细胞的低免疫原性和高增殖能力,在体内可能会存在恶性转化的潜在风险,通过影像学成像可以确定细胞治疗的安全性和有效性。
目的:需要详细的体内研究来监测移植细胞的命运,包括它们的分布、分化和寿命。实时追踪移植细胞的非侵入性细胞成像技术的进展,为确定干细胞治疗的有效性提供了一个强有力的工具。在这篇综述中,总结了最近10年干细胞标记和示踪使用的不同成像技术及其利弊。
方法:检索 PubMed 数据库、CNKI 数据库中2010年1月至2020年3月关于间充质干细胞非侵入性示踪技术的文章,英文检索词为“mesenchymal stem cell*;transplantation;cell track*;molecular imaging”,中文检索词为“间充质干细胞,移植,示踪”,检索文献类型不限,排除陈旧性和无关研究,最后选择92篇文献进行综述。
结果与结论:目前可用于活体干细胞示踪的分子影像技术主要包括光学成像、磁共振、核医学、超声/光声、磁粒子成像、多模态成像示踪等。间充质干细胞移植后的存活率和长期命运仍处于探索阶段,直接与间接标记成像相结合的新型多模态对比剂的出现,使得对移植细胞的分布和存活进行同时示踪成为可能。

https://orcid.org/0000-0002-3147-6824(李晔) 

中国组织工程研究杂志出版内容重点:干细胞;骨髓干细胞;造血干细胞;脂肪干细胞;肿瘤干细胞;胚胎干细胞;脐带脐血干细胞;干细胞诱导;干细胞分化;组织工程

关键词: 干细胞, 间充质干细胞, 细胞疗法, 干细胞移植, 回输, 活体示踪, 分子影像学, 多模态, 纳米颗粒

Abstract: BACKGROUND: Stem cell therapy has a broad application prospect for the development of new treatment strategies for many difficult diseases. Although the number of clinical trials of stem cell therapy has increased in the past few years, due to the low immunogenicity and high proliferation ability of stem cells, there may be a potential risk of malignant transformation in vivo. The safety and effectiveness of cell therapy can be determined by imaging.
OBJECTIVE: Detailed in vivo studies are needed to monitor the fate of transplanted cells, including their distribution, differentiation and lifespan. The development of non-invasive cell imaging techniques for real-time tracking of transplanted cells provides a powerful tool for determining the effectiveness of stem cell therapy. In this review, we summarize the advantages and disadvantages of different imaging techniques used for stem cell labeling and tracing in the last 10 years. 
METHODS: Articles about non-invasive tracer technology of mesenchymal stem cells in PubMed database and CNKI database from January 2010 to March 2020 were searched with the English key words of “mesenchymal stem cell*, cell track*, molecular imaging” and the Chinese key words of “mesenchymal stem cells, transplantation, tracer”. The search literature types were not limited; obsolete and irrelevant studies were excluded. Finally, 92 articles were selected for review. 
RESULTS AND CONCLUSION: At present, the molecular imaging techniques that can be used to trace stem cells in vivo mainly include optical imaging, magnetic resonance, nuclear medicine, ultrasound/photoacoustics, magnetic particle imaging, multimodal imaging and so on. The survival rate and long-term fate of mesenchymal stem cell transplantation are still in the exploratory stage. The emergence of the emergence of a variety of new multimodal contrast agents which are directly combined with indirect labeled imaging makes it possible to trace the distribution and survival of transplanted cells at the same time.

Key words: stem cells, mesenchymal stem cells, cell therapy, stem cell transplantation, transfusion, in vivo track, molecular imaging multimodal, nanoparticles

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