中国组织工程研究

中国组织工程研究 ›› 2012, Vol. 16 ›› Issue (8): 1363-1366.doi: 10.3969/j.issn.1673-8225.2012.08.008

• 纳米生物材料 nanobiomaterials • 上一篇    下一篇

金纳米链与人喉癌Hep-2细胞共培养自由进出细胞的形式*☆

丛林海1，何晓光1，杨一兵1，张世文2，彭淑昆1   

  1. 1昆明医学院第一附属医院耳鼻咽喉科，云南省昆明市  650031；2昆明医学院第三附属医院头颈外科，云南省昆明市  650031
  • 收稿日期:2011-07-16 修回日期:2011-08-26 出版日期:2012-02-19 发布日期:2012-02-19
  • 通讯作者: 何晓光，教授，昆明医学院第一附属医院耳鼻喉科，云南省昆明市 650031
  • 作者简介:丛林海☆，男，1972年生，黑龙江省伊春市人，汉族，2011年昆明医学院毕业，博士，主治医师，主要从事头颈肿瘤基础及临床研究。
  • 基金资助:

    国家自然科学基金项目(81160324/H1625)。课题名称：EGFR-mAb功能化修饰金纳米棒光热诱导Hep-2细胞凋亡分子机制研究。

     

Endocytosis and exocytosis of gold nanochain attaching Hep-2 cells of human laryngeal carcinoma*☆

Cong Lin-hai1, He Xiao-guang1, Yang Yi-bing1, Zhang Shi-wen2, Peng Shu-kun1   

  1. 1Department of Otorhinolaryngology, First Hospital of Kunming Medical University, Kunming 650031, Yunnan Province, China; 2Department of Head and Neck Surgery, Third Hospital of Kunming Medical University, Kunming 650031, Yunnan Province, China
  • Received:2011-07-16 Revised:2011-08-26 Online:2012-02-19 Published:2012-02-19
  • Contact: He Xiao-guang, Professor, Department of Otorhinolaryngology, First Hospital of Kunming Medical University, Kunming 650031, Yunnan Province, China hexg1018@ yahoo.com.cn
  • About author:Cong Lin-hai☆, Doctor, Attending physician, Department of Otorhinolaryngology, First Hospital of Kunming Medical University, Kunming 650031, Yunnan Province, China conglinhai2008@ 126.com
  • Supported by:

    the National Natural Science Foundation of China, No. 81160324/H1625*

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摘要：

背景：金纳米颗粒对肿瘤细胞具有杀伤效应。
目的：观察金纳米链对人喉癌Hep-2细胞增殖的影响。
方法：首先运用葡萄糖体系合成法制备金纳米链溶胶，然后MTT法检测不同终浓度(10%，25%，50%，75%，95%)金纳米链溶胶对人喉癌Hep-2细胞增殖的影响，并通过电镜观察金纳米链进出Hep-2细胞的过程。
结果与结论：金纳米链在75%和95%高浓度时对Hep-2细胞增殖有一定的抑制，但并没有随着浓度的增加而加重，均属于一级范围，表明金纳米链对人喉癌Hep-2细胞无毒性。金纳米链在与Hep-2细胞共培养8 h后即能以胞吞的方式进入细胞，48 h后大部分出胞，能够自由进出细胞。

关键词: 金纳米链, Hep-2细胞, 细胞毒性, 增殖, 生物材料与纳米技术

Abstract:

BACKGROUND: The gold nanoparticles have a killing effect on tumor cells.
OBJECTIVE: To investigate the influence of gold nanochain on Hep-2 cells proliferation of human laryngeal carcinoma.
METHODS: The gold nanochain was prepared by a glucose synthesis method and added into the culture cells with different concentrations (10%, 25%, 50%, 75%, 95%) to test the influence on proliferation of in vitro cultured Hep-2 cells. The endocytosis and exocytosis of transmembrane when gold nanochain attached to Hep-2 cells were observed by electron microscopy.
RESULTS AND CONCLUSION: The gold chain at high concentrations (75%, 95%) exhibited inhibitory effects on the proliferation of Hep-2 cells, but the influence was not increased with increasing concentration, belonging to a range of non-toxic. Gold nanchain can enter into Hep-2 cells after 8 hours of co-culture and leave cells after 48 hours, indicating gold nanoparticles chain can enter and leave Hep-2 freely.

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