中国组织工程研究

中国组织工程研究 ›› 2012, Vol. 16 ›› Issue (8): 1359-1362.doi: 10.3969/j.issn.1673-8225.2012.08.007

• 纳米生物材料 nanobiomaterials • 上一篇    下一篇

聚丁二炔纳米粒快速检测日本血吸虫**☆◆

周伟华1, 2，郭讯枝1, 2，张阳德1，何剪太1   

  1. 1中南大学国家卫生部肝胆肠外科研究中心，中南大学生物医学工程研究院，湖南省长沙市 410008；   2宜春学院江西省天然药物活性成分研究重点实验室，江西省宜春市 336000
  • 收稿日期:2011-12-08 修回日期:2011-12-20 出版日期:2012-02-19 发布日期:2012-02-19
  • 作者简介:周伟华☆，男，1971年生，广东省梅县人，汉族，副教授，2008年中南大学毕业，博士，主要从事纳米生物技术研究。 subdeb@ 163.com 郭讯枝☆，女，1972年生，湖南省韶山市人，汉族，中南大学在读博士，讲师，主要从事纳米生物技术研究。
  • 基金资助:

    江西省科技支撑计划资助项目(2009BSA12400) 和江西省教育厅科技项目(GJJ09359)。

Rapid detection of Schistosoma Japonicum by using polydiacetylene nanoparticles

Zhou Wei-hua1, 2, Guo Xun-zhi1, 2, Zhang Yang-de1, He Jian-tai1   

  1. 1National Hepatobiliary & Enteric Surgery Research Center, Ministry of Health, Institute of Biomedical Engineering of Central South University, Changsha  410008, Hunan Province, China; 2Key Laboratory of Jiangxi Province for Research on Active Ingredients in Natural Medicines, Yichun University, Yichun  336000, Jiangxi Province, China
  • Received:2011-12-08 Revised:2011-12-20 Online:2012-02-19 Published:2012-02-19
  • About author:Zhou Wei-hua☆, Doctor, Associate professor, National Hepatobiliary & Enteric Surgery Research Center, Ministry of Health, Institute of Biomedical Engineering of Central South University, Changsha 410008, Hunan Province, China; Key Laboratory of Jiangxi Province for Research on Active Ingredients in Natural Medicines, Yichun University, Yichun 336000, Jiangxi Province, China subdeb@163.com Guo Xun-zhi, Studying for master’s degree, Lecturer, National Hepatobiliary & Enteric Surgery Research Center, Ministry of Health, Institute of Biomedical Engineering of Central South University, Changsha 410008, Hunan Province, China; Key Laboratory of Jiangxi Province for Research on Active Ingredients in Natural Medicines, Yichun University, Yichun 336000, Jiangxi Province, China
  • Supported by:

     the Science Supporting Program of Jiangxi Province, No. 2009BSA12400*; the Science and Technology Project of Jiangxi Education Bureau, No. GJJ09359*

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摘要：

背景：采用PCR、ELISA、免疫荧光球法及基因芯片技术进行日本血吸虫的快速检测，需要数小时才能得到结果，并且对日本血吸虫有量的要求，还需配备专门人员、一定的仪器设备，故非常不适用于现场快速检验。
目的：制备聚丁二炔纳米粒，并对日本血吸虫进行免疫检测。
方法：采用超声分散法制备聚丁二炔纳米粒，并利用其检测日本血吸虫。
结果与结论：制备的聚丁二炔纳米粒粒径均匀，分布范围窄，结构稳定。取血吸虫感染兔血清稀释液滴入聚丁二炔免疫纳米粒稀释液中，溶液颜色由蓝变红，颜色变化约20 s时，体系有70%~80%发生了颜色变化，在约2 min体系颜色变化即基本完成。吸收光谱、透射电镜、激光粒径分布均显示，加入血吸虫感染兔血清后聚丁二炔免疫纳米粒变化很大。表明利用聚丁二炔免疫纳米粒比色检测日本血吸虫操作简便、快速。
关键词：聚丁二炔；纳米粒；PCDA；日本血吸虫；生物材料与纳米技术
doi:10.3969/j.issn.1673-8225.2012.08.007

关键词: 聚丁二炔, 纳米粒, PCDA, 日本血吸虫, 生物材料与纳米技术

Abstract:

BACKGROUND: PCR, ELISA, immunofluorescence-ball method and gene chip technology for rapid detection of Schistosoma Japonicum will need hours, volume requirements of Schistosoma Japonicum, specialists and certain equipments to get results, and are not suitable for on-site rapid test.
OBJECTIVE: To prepare polydiacetylene nanoparticles for detection of Schistosoma Japonicum.
METHODS: Polydiacetylene nanoparticles (PDA) were prepared using sonication.
RESULTS AND CONCLUSION: The prepared nanoparticles had uniform particle size, narrow distribution and stable structure. Dilution of rabbit serum after schistosome infection was dropped into PDA nanoparticle dilution, and the solution color turned from blue to red. Twenty seconds later, 70%-80% of the solution changed from blue to red. The whole solution changed in 2 minutes. The graphs of absorption spectroscopy, transmission electron microscopy, and nanosizer displayed big changes with Schistosoma Japonicum and antibody PDA nanoparticle. It is indicated that colorimetric detection of Schistosoma Japonicum using PDA nanoparticles is simple and rapid.

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