中国组织工程研究 ›› 2018, Vol. 22 ›› Issue (2): 222-227.doi: 10.3969/j.issn.2095-4344.0010

• 纳米生物材料 nanobiomaterials • 上一篇    下一篇

线粒体介导金银合金纳米粒子的合成

孟  彪,陈维毅,张益霞,温旭东,黎  凯
  

  1. 太原理工大学应用力学与生物医学工程研究所,山西省太原市  030024
  • 收稿日期:2017-08-22 出版日期:2018-01-18 发布日期:2018-01-18
  • 通讯作者: 陈维毅,博士,教授,博士生导师,太原理工大学应用力学与生物医学工程研究所,山西省太原市 030024
  • 作者简介:孟彪,男,1990年生,山西省运城市人,硕士,主要从事生物医学工程研究。
  • 基金资助:
    国家自然科学基金项目(81602506);山西省高等学校创新项目(2016143)

Mitochondria-mediated synthesis of gold-silver alloy nanoparticles

Meng Biao, Chen Wei-yi, Zhang Yi-xia, Wen Xu-dong, Li Kai
  

  1. Institute of Applied Mechanics and Biomedical Engineering, Taiyuan University of Technology, Taiyuan 030024, Shanxi Province, China
  • Received:2017-08-22 Online:2018-01-18 Published:2018-01-18
  • Contact: Chen Wei-yi, M.D., Professor, Doctoral supervisor, Institute of Applied Mechanics and Biomedical Engineering, Taiyuan University of Technology, Taiyuan 030024, Shanxi Province, China
  • About author:Meng Biao, Master, Institute of Applied Mechanics and Biomedical Engineering, Taiyuan University of Technology, Taiyuan 030024, Shanxi Province, China
  • Supported by:
    the National Natural Science Foundation of China, No. 81602506; the Innovative Program of Shanxi High Educations, No. 2016143

摘要:

文章快速阅读:

 

文题释义:
金属纳米材料:指三维空间中至少有一维处于纳米尺度或由它们作为基体单元构成的金属材料,按照维数可将金属纳米材料分为零维、一维和二维。金属纳米材料具有独特的力学性能、表面效应、小尺寸效应、久保效应、宏观量子隧道效应、原子扩散行为及催化和贮氢性能。
金属纳米粒子制备方法:主要通过肼类、有机醇、柠檬酸等强腐蚀性化学试剂和利用微生物合成方法,然而前者有一定生物毒性且生产成本高,后者操作复杂,生产周期较长;利用线粒体生物合成双金属金银纳米粒子是一种全新的纳米粒子绿色制备方法,方法简单,成本低,对生态环境友好。
 
背景:由于金银双金属纳米粒子较强的催化活性及选择性催化氧化的特性,在生物医学、药物传递、电化学分析等方面存在潜在的广泛应用。
目的:以动物细胞中线粒体为模板,制备金银合金纳米粒子。
方法:从鱼肝中分离线粒体;在1 mL偏碱性HAuCl4溶液(10 mmol/L)中加入1 mL线粒体母液,摇匀,加入1 mL AgNO3溶液(10 mmol/L),室温条件下在电磁搅拌器上反应20-30 h,观察反应液颜色从无色逐渐变为紫色,即得金银合金纳米粒子,对其进行表征。①细胞毒性实验:将不同质量浓度(0,25,50,75,100,125,150 mg/L)的金银合金纳米粒子加入到胃癌细胞中,培养48 h后,MTT法检测细胞增殖A值;②安全稳定性实验:将1 mL不同质量浓度(0,25,50,75,100,125,150 mg/L)的金银合金纳米粒子溶液置于玻璃反应瓶中,将0.2 mL超纯水分次加入到纳米粒子溶液中,左右震荡摇匀;当加入超纯水后,使用紫外-可见光谱对溶液进行表征。
结果与结论:①金银合金纳米粒子为合金结构,平均粒径20-30 nm,基本为球形分布,粒度较均一,结晶度较好,结构单一,表面存在羟基、羰基等活性基团;②MTT实验表明在150 mg/L质量浓度范围内,金银纳米粒子无明显的细胞毒性;③随着金银合金纳米粒子质量浓度的变化,强度最大的特征吸收峰与金银合金纳米颗粒的含量基本呈正相关;④结果表明,金银合金纳米粒子具有良好的细胞相容性及体外稳定性。

 ORCID: 0000-0002-5035-374X(孟彪)

关键词: 生物材料, 纳米材料, 纳米金, 纳米银, 生物相容性, 双金属纳米粒子, 线粒体, 一步法, 协同效应, 绿色化学, 可持续过程, 国家自然科学基金

Abstract:

BACKGROUND: Due to its strong catalytic activity and selective catalytic oxidation, gold-silver bimetallic nanoparticles have potential applications in biomedicine, drug delivery and electrochemical analysis.
OBJECTIVE: To prepare the gold-silver alloy nanoparticles using mitochondria in animal cells as templates.
METHODS: Separating mitochondria from the fish liver: 1 mL of mitochondrial mother liquor was added into 1 mL of alkaline HAuCl4 solution (10 mmol/L) and shaken; then, 1 mL of AgNO3 solution (10 mmol/L) was added, and the mixture was reacted in an electromagnetic stirrer for 20-30 hours until the color of the reaction solution was changed from colorless to purple, which indicated that gold and silver alloy nanoparticles were successfully obtained; finally, the characterization  of the nanoparticles was analyzed. (1) Cytotoxicity test: gold-silver alloy nanoparticles atdifferent concentrations (0, 25, 50, 75, 100, 125, 150 mg/L) were added into gastric cancer cells. After 48 hours of culture, the proliferation of cells (absorbance value) was detected by MTT assay. (2) Stability evaluation: 1 mL of gold-silver alloy nanoparticles at different concentrations (0, 25, 50, 75, 100, 125, 150 mg/L) were placed into glass reaction flasks and then, 0.2 mL of ultrapure water was added in portions following by shaking. After addition of the ultra-pure water, ultraviolet-visible spectrum was used to analyze the characterization of the solution.
RESULTS AND CONCLUSION: The gold-silver alloy nanoparticles were spherically distributed and confirmed to have an alloy structure with the average particle size of 20-30 nm, and presented to have the advantages of simple structure, good crystallinity, and active groups such as hydroxyl and carbonyl groups on the surface. MTT results showed that the gold-silver alloy nanoparticles at the mass concentration of < 150 mg/L had no obvious cytotoxicity. The characteristic absorption peak with the largest intensity was positively correlated with the concentration of gold-silver alloy nanoparticles. To conclude, the gold-silver alloy nanoparticles have good cytocompatibility and stability in vitro.

Key words: Metal Nanoparticles, Mitochondria, Tissue Engineering

中图分类号: