中国组织工程研究

中国组织工程研究 ›› 2021, Vol. 25 ›› Issue (22): 3540-3544.doi: 10.3969/j.issn.2095-4344.3234

• 材料生物相容性 material biocompatibility • 上一篇    下一篇

经聚多巴胺涂层的羟基磷灰石生物陶瓷具有亲水性与细胞黏附性

何  林1，吴  稀2，何  淞2，杨  森1   

  1. 1遂宁市中心医院口腔医学中心，四川省遂宁市   629000；2西南医科大学附属口腔医院，四川省泸州市   646000
  • 收稿日期:2020-07-10 修回日期:2020-07-11 接受日期:2020-08-11 出版日期:2021-08-08 发布日期:2021-01-20
  • 通讯作者: 杨森，博士，硕士生导师，副主任医师，遂宁市中心医院口腔医学中心，四川省遂宁市 629000
  • 作者简介:何林，女，1993年生，四川省会理县人，汉族，硕士，医师，主要从事口腔临床医学(口腔正畸学)研究。
  • 基金资助:
    中华口腔医学会西部行口腔医学临床科研基金(CSA-W-2016-01)，项目负责人：杨森；四川惠民口腔健康推广中心(SCHMKQ-KF-002)，项目负责人：杨森

Hydrophilicity and cell adhesion of hydroxyapatite bioceramics after the coating of polydopamine

He Lin1, Wu Xi2, He Song2, Yang Sen1   

  1. 1Dental Center of Suining Central Hospital, Suining 629000, Sichuan Province, China; 2Stomatological Hospital Affiliated to Southwest Medical University, Luzhou 646000, Sichuan Province, China
  • Received:2020-07-10 Revised:2020-07-11 Accepted:2020-08-11 Online:2021-08-08 Published:2021-01-20
  • Contact: Yang Sen, MD, Master’s supervisor, Associate chief physician, Dental Center of Suining Central Hospital, Suining 629000, Sichuan Province, China
  • About author:He Lin, Master, Physician, Dental Center of Suining Central Hospital, Suining 629000, Sichuan Province, China
  • Supported by:
    the Clinical Research Fund of Stomatology in Western China of Chinese Stomatological Association, No. CSA-W-2016-01 (to YS); the Sichuan Huimin Oral Health Promotion Center, No. SCHMKQ-KF-002 (to YS)

PDF

116

摘要：

文题释义：
聚多巴胺：可以通过多巴胺氧化自聚生成，是一种天然黑色素，具有良好的生物相容性，不会引起免疫排斥反应，而且其化学结构类似海洋贻贝分泌的黏蛋白，其中的邻苯二酚基团具有超强的黏附性能，可在多种材料表面甚至超疏水材料的表面进行超强黏附，已被应用于改善金属、陶瓷、半导体、纤维等多种有机材料和无机材料的表面性能。
人根尖乳头干细胞：为从人牙根尖尚未形成的第三磨牙牙根部牙乳头组织中分离出的成体干细胞，是一种间充质干细胞，与牙髓干细胞相比拥有更强的组织再生能力，与其他间充质干细胞相比其取材较方便，能变废为宝，更有临床应用价值。

背景：骨移植材料是目前研究的要点和难点，羟基磷灰石具有良好的生物相容性与降解性，但人工合成的羟基磷灰石机械性能差且缺乏骨诱导性能，将根尖乳头干细胞与临床广泛应用的骨修复材料羟基磷灰石生物陶瓷结合更接近于临床运用。
目的：观察羟基磷灰石生物陶瓷经聚多巴胺涂层后的理化性能和细胞毒性变化。
方法：将羟基磷灰石生物陶瓷置于多巴胺-Tris缓冲液中制备聚多巴胺涂层羟基磷灰石生物陶瓷，以未涂层羟基磷灰石生物陶瓷为对照，检测两种陶瓷的平均孔径、孔隙率和水滴接触角。将第3代人根尖乳头干细胞分别接种于两种陶瓷表面，培养15 min后，倒置显微镜与激光共聚焦显微镜下观察细胞黏附情况；培养1，3，5，7 d后，采用CCK-8法检测细胞毒性。
结果与结论：①涂层前后羟基磷灰石生物陶瓷的平均孔径、孔隙率比较差异无显著性意义(P > 0.05)，经聚多巴胺涂层后生物陶瓷表面的颗粒细屑更大，表面更加平整；②经聚多巴胺涂层生物陶瓷的接触角低于未涂层生物陶瓷(P < 0.001)；③倒置显微镜显示，未涂层生物陶瓷的细胞爬片周围散在分布人根尖乳头干细胞，聚多巴胺涂层的细胞爬片周围无散在细胞，人根尖乳头干细胞均匀黏附在其表面；激光共聚焦显微镜显示，与未涂层生物陶瓷的细胞爬片相比，聚多巴胺涂层细胞爬片表面的人根尖乳头干细胞体积更大、伸展更加充分，细胞触角更加明显；④两种陶瓷上培养不同时间点的人根尖乳头干细胞相对增殖率均在75%以上，细胞毒性0-1级；⑤结果表明与未涂层羟基磷灰石生物陶瓷相比，经聚多巴胺涂层羟基磷灰石生物陶瓷具有更好的亲水性与细胞黏附性。

关键词: 材料, 口腔, 聚多巴胺, 羟基磷灰石, 生物陶瓷, 人根尖乳头干细胞, 细胞毒性, 亲水性, 黏附

Abstract: BACKGROUND: Bone graft materials are the key points and difficulties in current research. Hydroxyapatite has good biocompatibility and degradation. However, the mechanical properties of synthetic hydroxyapatite are poor and lack of osteoinductive properties. It is more close to clinical application to combine the more easily obtained apical papilla stem cells with hydroxyapatite bioceramics, which is widely used in clinical bone repair.           
OBJECTIVE: To observe the physicochemical properties and cytotoxicity of hydroxyapatite bioceramics coated with polydopamine.           
METHODS: Polydopamine-hydroxyapatite bioceramics were prepared by placing hydroxyapatite bioceramics in dopamine Tris buffer solution. Uncoated hydroxyapatite bioceramics were used as control. The average pore diameter, porosity and contact angle of water droplets of polydopamine coated hydroxyapatite bioceramics and uncoated hydroxyapatite bioceramics were measured. The third generation of human apical papilla stem cells was inoculated on the two kinds of ceramic surfaces respectively. After 15 minutes of culture, the cell adhesion was observed under inverted microscope and laser confocal microscope. After 1, 3, 5 and 7 days of culture, the cytotoxicity was detected by CCK-8 assay.
RESULTS AND CONCLUSION: (1) There was no significant difference in the average pore size and porosity of hydroxyapatite bioceramics before and after coating (P > 0.05). The particles on the surface of polydopamine coated hydroxyapatite bioceramics were larger and the surface of polydopamine coated hydroxyapatite bioceramics was more flat. (2) The contact angle of polydopamine coated hydroxyapatite bioceramics was lower than that of uncoated hydroxyapatite bioceramics (P < 0.001). (3) Inverted microscope showed that human apical papilla stem cells were scattered around the uncoated cell slides, but not around the coated cell slides; and uniformly adhered human root tip papilla stem cells were observed on the surface of polydopamine coated cell slides. Laser confocal microscopy showed that compared with uncoated cell slides, the volume of human apical papilla stem cells on the surface of polydopamine coated cell climbing tablets was larger, more fully extended, and cell antennae were more obvious. (4) The relative proliferation rates of human apical papilla stem cells cultured at different time points on the two scaffolds were above 75%, and the cytotoxicities were 0-1 grade. (5) The results showed that compared with uncoated hydroxyapatite bioceramics, polydopamine coated hydroxyapatite bioceramics had better hydrophilicity and cell adhesion.

Key words: materials, oral cavity, polydopamine, hydroxyapatite, bioceramics, human apical papilla stem cells, cytotoxicity, hydrophilicity, adhesion

中图分类号: