中国组织工程研究

中国组织工程研究 ›› 2010, Vol. 14 ›› Issue (20): 3696-3700.doi: 10.3969/j.issn.1673-8225.2010.20.020

• 组织构建实验造模 experimental modeling in tissue construction • 上一篇    下一篇

建立荷人结肠癌裸鼠移植瘤模型及MRI成像检查

冯仕庭1，孙灿辉1，蔡华崧1，周 健1，谭国胜1，邱鹏新2，张 健3，李子平1，孟悛非1   

  1. 1中山大学附属第一医院放射科，广东省广州市  510080；
    中山大学，2药理学教研室， 3实验动物中心，广东省广州市  510080
  • 出版日期:2010-05-14 发布日期:2010-05-14
  • 通讯作者: 李子平，博士，教授，中山大学附属第一医院放射科，广东省广州市 510080 liziping163@tom.com
  • 作者简介:冯仕庭，男，1977年生，广东省新会市人，汉族，中山医科大学在读硕博连读研究生，主治医师，主要从事医学影像诊断方面的研究。
  • 基金资助:

    广东省科技计划项目(2007B031516007)

Establishment of human colonic carcinoma (LoVo) xenograft in nude mice and magnetic resonance imaging examination  

Feng Shi-ting1, Sun Can-hui1, Cai Hua-song1, Zhou Jian1, Tan Guo-sheng1, Qiu Peng-xin2, Zhang Jian3, Li Zi-ping1, Meng Quan-fei1   

  1. 1Department of Radiology, the First Affiliated Hospital, Sun Yat-sen University, Guangzhou  510080, Guangdong Province, China;
    2Department of Pharmacology, 3Laboratory Animal Center, Sun Yat-sen University, Guangzhou  510080, Guangdong Province, China
  • Online:2010-05-14 Published:2010-05-14
  • Contact: Li Zi-ping, Doctor, Professor, Department of Radiology, the First Affiliated Hospital, Sun Yat-sen University, Guangzhou 510080, Guangdong Province, China Liziping@tom.com
  • About author:Feng Shi-ting, Studying for doctorate, Attending physician, Department of Radiology, the First Affiliated Hospital, Sun Yat-sen University, Guangzhou 510080, Guangdong Province, China
  • Supported by:

    Technology Planning Project of Guangdong Province, No. 2007B031516007*

PDF

569

被引次数

18

摘要：

背景：肿瘤移植瘤动物模型是探索和模拟肿瘤在人体内生物学行为的一种较为理想的方法，但应用MRI对模型进行评价的研究较少。

目的：应用人结肠癌LoVo细胞接种裸鼠，鼠间移植传代，建立人结肠癌裸鼠移植瘤模型，并应用MRI进行初步评价。

方法：采用细胞移植和瘤块移植两种方法建立裸鼠移植瘤模型，观察移植瘤大体形态和组织病理学改变，测定宿主血液中的癌胚抗原值，应用免疫组化法观察肿瘤细胞中癌胚抗原的的分布，对6只裸鼠移植瘤模型进行MRI成像检查，并测量肿瘤、肝脏、肌肉组织的T1WI和T2WI的信噪比和三者的T1，T2值。 

结果与结论：移植瘤的形态和功能特性与原发肿瘤基本相似，移植瘤的移植成功率为100%；6只裸鼠模型的MRI测量结果显示，其肿瘤、肝脏、肌肉的T1WI平均信噪比分别为26.19，22.71，26.62；T2WI平均信噪比分别为9.42，7.66，8.59；平均T1值分别为1039.22，907.63，1611.51ms；平均T2值分别为109.95，37.31，64.35 ms。结果证实荷人结肠癌裸鼠模型的MRI成像图像清楚，组织分辨率高，测定组织的T1值和T2值可作为定量指标进行分析。

关键词: 肿瘤移植, 裸鼠, 结肠肿瘤, MRI, 动物模型

Abstract:

BACKGROUND: The colonic carcinoma xenograft model is an ideal method for exploring and stimulating in vivo biological behavior of human, however, studies concerning evaluating models using magnetic resonance imaging (MRI) have less been reported yet.

OBJECTIVE: To establish human colonic carcinoma xenograft model in nude mice with human colonic carcinoma LoVo cells inoculation and transplantation, and to evaluate the models by using MRI. 

METHODS: The nude mice models with colonic carcinoma were established by cell transplantation and tumor transplantation. The morphological and histopathological features of transplanted tumor were examined. Blood samples taken from the host were assayed for carcino embryonic antigen levels. Immunohistochemical localization of carcino embryonic antigen in the tumor tissues was also determined. The models (n = 6) were performed MRI examination. Signal to noise ratio (SNR) on T1 weighted imaging (T1WI) and T2 weighted imaging (T2WI), T1 and T2 relaxation time of the tumor, liver and muscle were measured.

RESULTS AND CONCLUSION: It showed that the morphological and functional characteristics of the transplanted tumors were strikingly identical to the original human tumor. The successful transplantation rate was 100%. The mean of SNR on T1WI of tumor, liver and muscle were 26.19, 22.71, and 26.62, respectively, and, the mean of SNR on T2WI were 9.42, 7.66 and 8.59, respectively. T1 relaxation time of tumor, liver and muscle were 1 039.22 ms, 907.63 ms and 1 611.51 ms, respectively, while the mean of T2 relaxation time were 109.95 ms, 37.31 ms and 64.35 ms, respectively. MRI was performed successfully in all the models with good imaging quality and high resolution. T1 and T2 relaxation time could be the quantitative index for further evaluation.

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