中国组织工程研究 ›› 2021, Vol. 25 ›› Issue (13): 2079-2086.doi: 10.3969/j.issn.2095-4344.2193
• 干细胞综述 stem cell review • 上一篇 下一篇
韩宁宁,左进富,孙 淼,唐胜建,刘方军
收稿日期:
2020-05-11
修回日期:
2020-05-16
接受日期:
2020-06-17
出版日期:
2021-05-08
发布日期:
2020-12-29
通讯作者:
刘方军,教授,硕士生导师,潍坊医学院整形外科研究所,山东省潍坊市 261000
作者简介:
韩宁宁,女,1994年生,山东省梁山县人,汉族,潍坊医学院在读硕士,主要从事骨缺损修复方面的研究。
Han Ningning, Zuo Jinfu, Sun Miao, Tang Shengjian, Liu Fangjun
Received:
2020-05-11
Revised:
2020-05-16
Accepted:
2020-06-17
Online:
2021-05-08
Published:
2020-12-29
Contact:
Liu Fangjun, Professor, Master’s supervisor, Plastic Surgery Institute, Weifang Medical University, Weifang 261000, Shandong Province, China
About author:
Han Ningning, Master candidate, Plastic Surgery Institute, Weifang Medical University, Weifang 261000, Shandong Province, China
摘要:
文题释义:
人脐带间充质干细胞:是源于新生儿脐带组织的一种多功能干细胞,具备较强的增殖、自我更新能力。同时,由于其具有多向分化潜能,成为骨、软骨、肌肉、肌腱、神经、肝、心肌等组织工程种子细胞的优良选择。目前,可从整条脐带及脐带不同组织(羊膜上皮细胞、Wharton’s jelly及脐动静脉)分别提取出间充质干细胞。
生物打印技术:采用特殊打印装置,对生物材料、活细胞及生长因子等活性因子进行程序化打印,可精确调控宏观及微观结构,最终得到三维立体组织、器官等仿生结构。此方法为细胞生物学、再生医学和组织工程重建等领域做出重要贡献。
背景:由于脐带间充质干细胞作为种子细胞具有来源广泛、取材方便、免疫原性低、成骨分化潜能及较强的增殖和自我更新能力等诸多优点,近年来关于其在骨组织工程中的应用研究越来越多。
目的:对脐带间充质干细胞在分离培养、成骨诱导及其支架等方面进行综述。
方法:由第一作者检索CNKI数据库和PubMed数据库的相关文章,检索词为“人脐带间充质干细胞,分离,培养,成骨分化,支架,骨组织工程”“human umbilical cord stem cell,culture,isolation,osteogenic differentiation,scaffold,bone tissue engineering”,查阅2004至2020年来有关的文献,最终纳入104篇文献。
结果与结论:脐带间充质干细胞的分离、培养方法不一;无血清或动物血清替代物培养体系及细胞共培养技术取得了很大进展;3D培养系统将是未来发展的方向。脐带间充质干细胞成骨诱导分化机制仍不明确,需深入研究阐明。至今,开发取长补短、性能更优的复合支架仍是研究的焦点;生物打印技术突破了微米级精确调控支架内部复杂结构打造个体化支架的难题,为骨组织工程带来巨大希望;设计和制造出具有多方面理想组成(包括生物相容性、微观及宏观高孔隙率、机械性能和相关生物可吸收性等)及减少临床治疗不良反应的支架仍是骨组织工程中的重要挑战之一。
中国组织工程研究杂志出版内容重点:干细胞;骨髓干细胞;造血干细胞;脂肪干细胞;肿瘤干细胞;胚胎干细胞;脐带脐血干细胞;干细胞诱导;干细胞分化;组织工程
中图分类号:
韩宁宁, 左进富, 孙 淼, 唐胜建, 刘方军. 脐带间充质干细胞在骨组织工程中的应用与进展[J]. 中国组织工程研究, 2021, 25(13): 2079-2086.
Han Ningning, Zuo Jinfu, Sun Miao, Tang Shengjian, Liu Fangjun. Application and progress of umbilical cord mesenchymal stem cells in bone tissue engineering[J]. Chinese Journal of Tissue Engineering Research, 2021, 25(13): 2079-2086.
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中国组织工程研究杂志出版内容重点:干细胞;骨髓干细胞;造血干细胞;脂肪干细胞;肿瘤干细胞;胚胎干细胞;脐带脐血干细胞;干细胞诱导;干细胞分化;组织工程
中国组织工程研究杂志出版内容重点:干细胞;骨髓干细胞;造血干细胞;脂肪干细胞;肿瘤干细胞;胚胎干细胞;脐带脐血干细胞;干细胞诱导;干细胞分化;组织工程
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