中国组织工程研究

中国组织工程研究 ›› 2016, Vol. 20 ›› Issue (50): 7557-7564.doi: 10.3969/j.issn.2095-4344.2016.50.016

• 干细胞移植 stem cell transplantation • 上一篇    下一篇

高压氧对慢性应激抑郁模型大鼠行为及神经干细胞移植的影响

米  琨1，郭  强2，桑文华1，王海波2，李  乐3   

  1. 1河北省第六人民医院情感障碍二科，河北省保定市  071000
    2河北大学附属医院胸外科，河北省保定市  071000
    3保定市第一中心医院，河北省保定市  071028
  • 修回日期:2016-09-28 出版日期:2016-12-02 发布日期:2016-12-02
  • 通讯作者: 郭强，主治医师，河北大学附属医院胸外科，河北省保定市071000
  • 作者简介:米琨，女，1981年生，河北省保定市人，汉族，2006年河北医科大学毕业，主治医师，主要从事精神卫生疾病的临床与基础研究。

Effects of hyperbaric oxygen on behavior and transplanted neural stem cells in rats with chronic stress depression

Mi Kun1, Guo Qiang2, Sang Wen-hua1, Wang Hai-bo2, Li Le3   

  1. 1Department of Affective Disorder, Sixth People’s Hospital of Hebei Province, Baoding 071000, Hebei Province, China
    2Department of Thoracic Surgery, Affiliated Hospital of Hebei University, Baoding 071000, Hebei Province, China
    3Baoding First Central Hospital, Boading 071028, Hebei Province, China
  • Revised:2016-09-28 Online:2016-12-02 Published:2016-12-02
  • Contact: Guo Qiang, Attending physician, Department of Thoracic Surgery, Affiliated Hospital of Hebei University, Baoding 071000, Hebei Province, China
  • About author:Mi Kun, Attending physician, Department of Affective Disorder, Sixth People’s Hospital of Hebei Province, Baoding 071000, Hebei Province, China

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摘要：

文章快速阅读：

文题释义：
神经干细胞：具有分化为神经神经元、星形胶质细胞和少突胶质细胞的能力，能自我更新，并足以提供大量脑组织细胞的细胞群；是一类具有分裂潜能和自更新能力的母细胞，它可以通过不对等的分裂方式产生神经组织的各类细胞。需要强调的是，在脑脊髓等所有神经组织中，不同的神经干细胞类型产生的子代细胞种类不同，分布也不同。
高压氧：是指机体处于高气压环境中所呼吸的与环境等压的纯氧，而利用吸入高压氧治疗疾病的方法称为高压氧疗法。高压氧是目前最经济且不会造成伤害的供氧方式，其他任何方法都不能代替其地位。尽管如此，单独应用高压氧进行疾病治疗的情况极为少见，通常要根据疾病的需要，联合其他手段进行综合性治疗，以取得更好的疗效。

 

摘要
背景：高压氧治疗缺血缺氧性疾病的效果已得到公认，但需要与其他手段进行联合治疗。
目的：观察高压氧联合神经干细胞移植治疗大鼠慢性应激抑郁的效果。
方法：将60只SD大鼠随机分为4组，正常组不进行任何处理；神经干细胞组、联合组、模型组建立慢性应激抑郁模型。造模成功后24 h，联合组尾静脉注射神经干细胞悬液1 mL(细胞数3×106)，同时给予高压氧治疗，4次/d，持续1周；神经干细胞组尾静脉注射神经干细胞悬液1 mL(细胞数3×106)。造模成功后72 h，TUNEL法测定神经细胞凋亡情况；治疗后2周，观察神经干细胞的存活和分布情况，尼氏染色法检测大鼠海马神经元形态；治疗后1，2周，进行体质量测量、蔗糖水消耗实验；治疗后5 min内进行旷场实验，观察大鼠行为学改变。
结果与结论：①与正常组比较，模型组大鼠体质量、蔗糖水消耗量、矿场实验垂直直立次数与水平运动距离明显减少(P <  0.05)，神经细胞凋亡数量明显增加(P < 0.05)；②与模型组比较，神经干细胞组、联合组大鼠体质量、蔗糖水消耗量、矿场实验垂直直立次数与水平运动距离明显升高(P < 0.05)，神经细胞凋亡数量明显减少(P < 0.05)，联合组的效果优于神经干细胞组(P < 0.05)；③联合组移植神经干细胞存活数量明显多于神经干细胞组(P < 0.05)；④模型组神经元数目相对较少，结构不完整；神经干细胞组及联合组神经元数目多，结构完整；联合组神经元数目较神经干细胞组多，但少于正常组；⑤结果表明，神经干细胞联合高压氧可改善慢性应激抑郁模型大鼠的抑郁行为及海马神经元凋亡情况。

 

 

关键词: 干细胞, 移植, 神经干细胞, 高压氧, 抑郁, 大鼠

Abstract:

BACKGROUND: Hyperbaric oxygen as a well-recognized therapy for ischemic and hypoxic diseases has to be combined with other treatments.
OBJECTIVE: To study the effects of hyperbaric oxygen combined with neural stem cell transplantation via tail vein in chronic stress depression rats.
METHODS: Of 60 Sprague-Dawley rats, 15 rats randomly selected were given no treatment as normal group, and the rest 45 ones were used to establish a rat model of depression and randomly divided into three groups: model group (n=15, without giving any treatment), neural stem cell group (n=15, injection of 1 mL neural stem cell suspension (3×106) via the tail vein) and combined group (n=15, hyperbaric oxygen treatment plus neural stem cell injection). Hyperbaric oxygen treatments were carried out four times per day, for 1 week, and all the treatments were given 24 hours after modeling. Rats in each group were subjected to body mass measurement and sugar water consumption test 1 and 2 weeks after treatment, and open field test within 5 minutes after treatment to observe rat behavior changes. The survival and distribution of CM-Dil labeled neural stem cells were observed by fluorescence microscopy. The changes in the size, morphology and number of hippocampus neurons in rat were detected by the method of nylon staining. TUNEL method was used to measure the apoptosis of nerve cells.
RESULTS AND CONCLUSION: Compared with the normal group, in the model group, rat’s body weight, sucrose water preference and open field test scores were significantly lower, reduced number of hippocampal neurons with intact structure was found and TUNEL results showed more apoptosis (P < 0.05). Compared with the model group, the body weight, sucrose preference and open field test score increased significantly (P < 0.05), the number of hippocampal neurons was increased, while the number of apoptotic cells was reduced significantly in the neural stem cell and combined groups (P < 0.05). Compared with the neural stem cell group, these indexes were improved more significantly in the combined group (P < 0.05). More CM-Dil positive cells were found in the combined group than the neural stem cell group (P < 0.05). Additionally, the number of hippocampal neurons was higher in the combined group than the neural stem cell group, but lower than the normal group. To conclude, transplantation of neural stem cells combined with hyperbaric oxygen can improve the depression behavior and apoptosis in the hippocampal neurons in chronic stress depression rats.

 

 

Key words:  Stem Cells, Stem Cell Transplantation, Tissue Engineering

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