中国组织工程研究 ›› 2016, Vol. 20 ›› Issue (50): 7565-7570.doi: 10.3969/j.issn.2095-4344.2016.50.017

• 干细胞培养与分化 stem cell culture and differentiation • 上一篇    下一篇

酶消化法和组织块法培养心肌干细胞的比较

李  沙,李树仁,郝清卿,杨玲玲,张倩辉,马玉龙,张跃华,邓文浩   

  1. 河北医科大学附属河北省人民医院心内一科,河北省石家庄市  050051
  • 修回日期:2016-10-17 出版日期:2016-12-02 发布日期:2016-12-02
  • 通讯作者: 李树仁,博士,教授,主任医师,硕士生导师,河北省人民医院心内一科,河北省石家庄市 050051
  • 作者简介:李沙,女,1986年生,河北省邯郸县人,汉族,河北医科大学在读硕士,主要从事心血管病研究。
  • 基金资助:

    河北省自然科学基金项目(C2015307019)

Enzyme digestion method versus tissue block method in the culture of cardiac stem cells

Li Sha, Li Shu-ren, Hao Qing-qing, Yang Ling-ling, Zhang Qian-hui, Ma Yu-long, Zhang Yue-hua, Deng Wen-hao   

  1. Department of Cardiology, Hebei General Hospital Affiliated to Hebei Medical University, Shijiazhuang 050051, Hebei Province, China
  • Revised:2016-10-17 Online:2016-12-02 Published:2016-12-02
  • Contact: Li Shu-ren, M.D., Professor, Chief physician, Master’s supervisor, Department of Cardiology, Hebei General Hospital, Shijiazhuang 050051, Hebei Province, China
  • About author:Li Sha, Studying for master’s degree, Department of Cardiology, Hebei General Hospital Affiliated to Hebei Medical University, Shijiazhuang 050051, Hebei Province, China
  • Supported by:

    the Natural Science Foundation of Hebei Province, No. C2015307019

摘要:

文章快速阅读:

文题释义:
心肌干细胞:
成年哺乳动物心脏内存在心肌干细胞巢,在心房和心尖部位分布较多,心肌干细胞除存在于心肌干细胞巢外,也有少数分散在心肌中,心脏受到损伤刺激后,能够迅速向损伤部位迁移、分化产生心肌细胞。心肌干细胞的主要生物学特性包括:①克隆能力;②有多能性和能够自我更新;③能够分化为成熟的心肌细胞、血管内皮细胞及平滑肌细胞等心脏结构细胞;④可在损伤心肌局部激活、迁移。
免疫细胞化学:又称免疫组织化学,其主要原理是用标记的抗体或抗原对细胞相应抗原或抗体进行定性、定位或定量检测,经过化学的呈色反应后,用显微镜或电子显微镜观察。

 

摘要
背景:
目前国内动物研究中提取心肌干细胞方法不统一,分离培养及提纯未形成规范化的流程。
目的:探讨酶消化法和组织块法培养大鼠心肌干细胞的差异。
方法:取清洁级1 d龄SD大鼠心脏组织,分为组织块组与酶消化组,分别使用酶消化法和组织块法体外培养心肌干细胞。
结果与结论:酶消化法和组织块法均能培养出心肌干细胞,但组织块组培养的细胞中心肌干细胞比例显著高于酶消化组。提示在心肌干细胞培养过程中,组织块法优于酶消化法。

 

 

关键词: 干细胞, 培养, 心肌干细胞, 细胞培养, 免疫荧光, 流式细胞术, 免疫组化, C-kit+心肌干细胞, 河北省自然科学基金

Abstract:

BACKGROUND: At present, various methods of extracting cardiac stem cells have been reported in China, but the processes of isolation, culture and purification are not yet standardized.
OBJECTIVE: To explore the difference in the culture of rat cardiac stem cells using enzyme digestion method and tissue block method.
METHODS: Cardiac stem cells from Sprague-Dawley rats, clean grade, 1 day of age, were extracted and cultured in vitro using the enzyme digestion method (control group) or tissue block method (experimental group).
RESULTS AND CONCLUSION: Both of the two methods could produce cardiac stem cells, but the proportion of cultured cells in the experimental group was significantly higher than that in the control group, indicating the tissue block method is superior to the enzyme digestion method in the culture of cardiac stem cells.

 

 

Key words: Cell Culture Techniques, Myocardium, Tissue Engineering

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