中国组织工程研究

中国组织工程研究 ›› 2014, Vol. 18 ›› Issue (16): 2563-2569.doi: 10.3969/j.issn.2095-4344.2014.16.017

• 生物材料基础实验 basic experiments of biomaterials • 上一篇    下一篇

淫羊藿苷抑制钛颗粒诱导的炎症反应

崔京福，徐耀增，朱世军，朱 锋，付 稳，邵洪国，耿德春   

  1. 苏州大学附属第一医院骨科，江苏省苏州市  215006
  • 修回日期:2014-03-10 出版日期:2014-04-16 发布日期:2014-04-16
  • 通讯作者: 耿德春，副研究员，苏州大学附属第一医院骨科，江苏省苏州市 215006
  • 作者简介:崔京福，男，1988年生，河南焦作孟州市人，汉族，苏州大学附属第一医院在读硕士，主要从事骨关节外科研究。
  • 基金资助:

    江苏省临床医学科技专项资助项目(BL2012004)；国家自然科学基金资助项目(81101399，81272018，81372018)；江苏省自然科学基金资助项目(BK2011303)；江苏省研究生培养创新工程资助项目(CXZZ13_ 0835)

Icariin inhibits titanium particle-induced inflammatory reaction

Cui Jing-fu, Xu Yao-zeng, Zhu Shi-jun, Zhu Feng, Fu Wen, Shao Hong-guo, Geng De-chun   

  1. Department of Orthopaedics, the First Affiliated Hospital of Soochow University, Suzhou 215006, Jiangsu Province, China
  • Revised:2014-03-10 Online:2014-04-16 Published:2014-04-16
  • Contact: Geng De-chun, Associate investigator, Department of Orthopaedics, the First Affiliated Hospital of Soochow University, Suzhou 215006, Jiangsu Province, China
  • About author:Cui Jing-fu, Studying for master’s degree, Department of Orthopaedics, the First Affiliated Hospital of Soochow University, Suzhou 215006, Jiangsu Province, China
  • Supported by:

    the Clinical Medicine Specific Fund of Jiangsu Province, No. BL2012004; the National Natural Science Foundation of China, No. 81101399, 81272018, 81372018; the Natural Science Foundation of Jiangsu Province, No. BK2011303; the Innovation Project for Graduate Culture in Jiangsu Province, No. CXZZ13_0835

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摘要：

背景：体外实验表明，淫羊藿苷可抑制脂多糖诱导的急性肺炎，其抗炎作用在磨损颗粒存在的条件下是否依然有效？

目的：通过体内外实验相结合的方法探讨淫羊藿苷对磨损颗粒诱导炎症反应的调控作用。
方法：①体内实验：将80只雄性C57BL/6小鼠随机分为4组，钛组和钛+淫羊藿苷组采用钛诱导小鼠颅骨无菌炎症模型，对照组、淫羊藿苷组也进行相同手术过程，但不植入钛，淫羊藿苷组、钛+淫羊藿苷组建模当天灌胃给予淫羊藿苷200 mg/(kg·d)，对照组、钛组灌胃给予等量的安慰剂，2周后酶联免疫吸附实验、定量RT-PCR检测肿瘤坏死因子α，白细胞介素1β蛋白或mRNA的表达量。②体外实验：将小鼠单核/巨噬细胞RAW264.7分别与核因子кB受体配体、核因子кB受体配体+淫羊藿苷、核因子кB受体配体+钛颗粒及核因子кB受体配体+钛颗粒+淫羊藿苷共培养72 h，ELISA检测培养基上清中肿瘤坏死因子α，白细胞介素1β水平，RT-PCR分析肿瘤坏死因子α，白细胞介素1β基因mRNA表达。

结果与结论：①体内实验：在钛颗粒存在的条件下，口服淫羊藿苷可明显减少颗粒诱导的炎症细胞浸润，使炎性增厚的骨膜变薄，抑制颅骨标本中肿瘤坏死因子α，白细胞介素1β的表达。②体外实验：经钛颗粒刺激后，细胞培养基中肿瘤坏死因子α，白细胞介素1β质量浓度显著增加，细胞中相应mRNA表达量上调，而经淫羊藿苷干预后这两种炎性因子在蛋白和基因水平的表达量显著下调。结果表明淫羊藿苷在体内外均可显著抑制钛颗粒诱导的炎症反应。


中国组织工程研究杂志出版内容重点：生物材料；骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性；组织工程


全文链接：

关键词: 生物材料, 材料相容性, 淫羊藿苷, 钛颗粒, 炎症反应, 肿瘤坏死因子α, 白细胞介素1β, 国家自然科学基金

Abstract:

BACKGROUND: Studies in vitro have suggested that icariin can attenuate lipopolysaccharide (LPS)-induced acute pneumonia. Is the anti-inflammatory effect of icariin still valid in the presence of wear particles?

OBJECTIVE: With studies in vivo and in vitro, to investigate the regulatory effect of icarrin on titanium particle-induced inflammatory reaction.
METHODS: (1) Studies in vivo: Eighty male C57BL/6 mice aged 6-8 weeks were randomly divided into four groups: control group, icariin group, titanium particle group, and titanium particle+icariin group. Mice in the titanium particle group and titanium particle+icariin group received surgical procedure, and sterile and endotoxin-free titanium particles were implanted on the calvaria surfaces to induce inflammatory reaction. Mice in the control group and icariin group received the same surgery, but no wear particles were implanted. Then icariin was given orally to mice in the titanium particle group and titanium particle+ icariin group with a dose of 200 mg/kg per day for 2 weeks from the day of modeling. Mice in the control group and icariin group were given orally the same dose of placebo. Two weeks later, tumor necrosis factor-α and interleukin-1β at protein and mRNA levels were respectively detected with enzyme-linked immunohistochemical (ELISA) and quantitative real time reverse transcription PCR (qRT-PCR) analysis. (2) Studies in vitro: Mouse monocyte/macrophage RAW264.7 cells were cultured with different conditioned media: control group, nuclear factor receptor ligand кB (RANKL); icariin group, RANKL+icariin; titanium particle group, RANKL+titanium particles; titanium particle+icarrin group, RANKL+icariin+titanium particles. Titanium particles stimulated RAW264.7 cells were co-cultured with RANKL and icariin for 72 hours. Tumor necrosis factor-α and interleukin-1β at protein and mRNA levels in the supernatant were detected with ELISA analysis and qRT-PCR, respectively.

RESULTS AND CONCLUSION: (1) Results in vivo: icariin treatment obviously decreased titanium particle-induced inflammatory cell infiltration and made the thickness of periosteum thinner, down-regulated tumor necrosis factor-α and interleukin-1β expressions at protein and mRNA levels. (2) Results in vitro: when RAW264.7 cells were stimulated with titanium particles for 72 hours, tumor necrosis factor-α and interleukin-1β expressions at protein and mRNA levels in culture media increased obviously; when icariin was administrated, tumor necrosis factor-α and interleukin-1β expressions at protein and mRNA levels down-regulated significantly. These results suggest icariin can obviously suppress titanium particle-induced inflammatory reaction in vivo and in vitro.


中国组织工程研究杂志出版内容重点：生物材料；骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性；组织工程


全文链接：

Key words:  titanium, systemic inflammatory response syndrome, drugs, chinese herbal, tumor necrosis factor-alpha, interleukin-1beta

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