关键词: 组织构建, 组织构建实验造模, 高血压, 肺性, 肺动脉高压模型, 脱氢野百合碱, 野百合碱, Swan-Ganz漂浮导管, Vigilance系统, 连续热稀释法, 肺血管阻力, 心输出量, 省级基金

Abstract:

BACKGROUND: Previous methods of invasive blood pressure monitoring in small animals with pulmonary hypertension are mediated by pressure tracings of biological signals acquisition system using right heart  catheter method, however, due to the limitations of experimental instruments and animal size, pulmonary vascular resistance and cardiac output cannot be measured with pulmonary artery catheter method.
OBJECTIVE: To measure cardiac output and pulmonary vascular resistance in beagle models with pulmonary hypertension induced by dehydromonocrotaline by using Swan-Ganz seven-lumen floating catheter and Vigilance monitor system with principles of continuous thermodilution method, and to explore the application value of continuous cardiac output method in pulmonary hypertension models.
METHODS: Ten beagles were randomized into two groups: control group (right atrium was injected with dimethylformamide), and experimental group (right atrium was injected with dehydromonocrotaline to establish pulmonary hypertension models). Prior to the administration and 8 weeks after administration, the right atrial systolic pressure, right ventricular systolic pressure, pulmonary arterial systolic pressure, mean pulmonary arterial pressure, pulmonary capillary wedge pressure and cardiac output of beagles in two groups were measured using Swan-Ganz floating catheter and Vigilance monitor system.
RESULTS AND CONCLUSION: In the experimental group, the pulmonary vascular resistance was significantly increased (P=0.00), while cardiac output was significantly decreased (P < 0.05) at 8 weeks after dehydromonocrotaline injection. Continuous thermodilution method was superior to conventional interrupted thermodilution method. We concluded that floating catheter and Vigilance monitor system based on the principles of continuous thermodilution method is an accurate, stable, and repeatable method for measuring pulmonary vascular resistance and cardiac output in beagle models with pulmonary hypertension induced by dehydromonocrotaline, with less trauma.

Key words:  tissue construction, experimental modeling in tissue construction, hypertension, pulmonary, pulmonary hypertension model, dehydromonocrotaline, monocrotaline, Swan-Ganz floating catheter, Vigilance system, continuous thermodilution method, pulmonary vascular resistance, cardiac output, provincial grants-supported paper