中国组织工程研究

中国组织工程研究 ›› 2012, Vol. 16 ›› Issue (28): 5185-5190.doi: 10.3969/j.issn.2095-4344.2012.28.012

• 组织构建实验造模 experimental modeling in tissue construction • 上一篇    下一篇

Ⅲ期压疮豚鼠模型的构建

郑 敏，甘秀妮，胡亚丽   

  1. 重庆医科大学附属第二医院护理部，重庆市 400010
  • 收稿日期:2012-02-23 修回日期:2012-05-02 出版日期:2012-07-08 发布日期:2012-07-08
  • 通讯作者: 甘秀妮，主任护师，硕士导师，重庆医科大学附属第二医院护理部，重庆市 400010 ganxn@163.com
  • 作者简介:郑敏★，女，1979年生，重庆医科大学在读硕士，主要从事临床护理方面的研究。 1287285766@qq.com

Construction of an animal model for stage Ⅲ pressure ulcer in guinea pigs

Zheng Min, Gan Xiu-ni, Hu Ya-li   

  1. Department of Nursing, the Second Affiliated Hospital of Chongqing Medical University, Chongqing 400010, China
  • Received:2012-02-23 Revised:2012-05-02 Online:2012-07-08 Published:2012-07-08
  • Contact: Gan Xiu-ni, Chief nurse, Master’s supervisor, Department of Nursing, the Second Affiliated Hospital of Chongqing Medical University, Chongqing 400010, China ganxn@163.com
  • About author:Zheng Min★, Studying for master’s degree, Department of Nursing, the Second Affiliated Hospital of Chongqing Medical University, Chongqing 400010, China

PDF

312

被引次数

5

摘要：

背景：压疮的发生发展是一个由多种原因引起的复杂病理过程，加之受到伦理道德和法律上的限制，无法直接在人体上进行实验研究。
目的：建立Ⅲ期压疮豚鼠模型，为压疮预防、诊断、治疗和发病机制提供研究依据。
方法：根据缺血-再灌注原理，利用自制建模装置对豚鼠(n=3)左侧大转子处间歇性施加不同压力6.65，13.30，19.95，26.60，33.25，39.90 kPa循环，诱导Ⅲ期压疮的形成，从大体、组织病理和行为学3个方面对模型进行评价。
结果与结论：各实验组创面颜色及形态学特征相似，但出现时间与压强大小成反比。组织苏木精-伊红染色显示不同程度的病理损害特征，6.65 kPa组<13.30 kPa组，19.95 kPa组<33.25，39.90 kPa组，其中13.30 kPa压力时组织病理表现与人类Ⅲ期压疮最为近似。与对照组比较，施加不同压力的各组豚鼠饮食量、饮水量及体质量变化比较，差异有显著性意义(P < 0.01)；随着施加的压强增加，豚鼠外观、精神状态、步态和性情逐步出现异常，其中6.65，13.30 kPa组豚鼠行为异常最少。说明在13.30 kPa压强下，采取缺血再灌注方式循环反复施加于豚鼠左侧大转子处，每天循环4次，连续12次(3 d)，后接5 d硬痂下感染溶解期，可稳定重现Ⅲ期压疮创面，且对豚鼠行为学影响较小，可用于需长期观察的压疮研究。

关键词: 压疮, 创面, 形态学, 豚鼠, 动物模型, 组织构建

Abstract:

BACKGROUND: The occurrence and development of pressure ulcers is a complex pathological process caused by a variety of reasons, coupled by the ethical and legal constraints. The relevant experiments cannot be directly done in the human body.
OBJECTIVE: To construct an animal model for stage Ⅲ pressure ulcer in guinea pigs, which can provide evidence for studies on prevention, diagnosis, treatment and pathogenesis of pressure ulcer.
METHODS: According to the ischemia-reperfusion principle, stage Ⅲ pressure ulcer was induced on the left trochanter of guinea pigs by exerting different pressures (6.65, 13.3, 19.95, 26.60, 33.25, 39.90 kPa). The model was evaluated based on general, pathohistological and behavioral observation.
RESULTS AND CONCLUSION: Wound color and morphological features were similar among different groups, but appearing time was inversely proportional to the pressure size. Hematoxylin-eosin staining showed different degrees of histopathological injuries (6.65 kPa < 13.30, 19.95 kPa < 33.25, 39.90 kPa). When the pressure was 13.30 kPa, the pathohistological presence of the animal models was most similar to that of stage Ⅲ pressure ulcer in the human body. Compared with the control group, the experimental groups had a significant difference in food intake, water intake and body mass changes from the control group (P < 0.01). With the increase in the pressure applied, the appearance, mental status, gait and temperament of guinea pigs became abnormal gradually. Stage Ⅲ pressure ulcer model can be prepared under the pressure of 13.30 kPa on the left trochanter by the ischemia-reperfusion method, four cycles a day, 12 cycles in a row (3 days) followed by 5 days without treatment. This model can be used for long-term observation of the pressure ulcer study.

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