中国组织工程研究

中国组织工程研究 ›› 2012, Vol. 16 ›› Issue (28): 5181-5184.doi: 10.3969/j.issn.2095-4344.2012.28.011

• 组织构建实验造模 experimental modeling in tissue construction • 上一篇    下一篇

育亨宾、纳洛酮及两药合用对氯胺酮麻醉模型小鼠的催醒试验

陈 琳，张彩丽，鞠艳敏，周振雷   

  1. 南京农业大学动物医学院，江苏省南京市 210095
  • 收稿日期:2012-01-15 修回日期:2012-02-11 出版日期:2012-07-08 发布日期:2012-07-08
  • 通讯作者: 周振雷，博士，副教授，南京农业大学动物医学院，江苏省南京市 210095
  • 作者简介:陈琳，女，1992年生，安徽省滁州市人，汉族，南京农业大学动物医学院在读本科生。 njauchenlin@163.com

Effect of yohimbine, naloxone and their combination on analepsia from ketamine-induced anesthesia in mice

Chen Lin, Zhang Cai-li, Ju Yan-min, Zhou Zhen-lei   

  1. College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, Jiangsu Province, China
  • Received:2012-01-15 Revised:2012-02-11 Online:2012-07-08 Published:2012-07-08
  • Contact: Zhou Zhen-lei, Doctor, Associate professor, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, Jiangsu Province, China
  • About author:Chen Lin, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, Jiangsu Province, China njauchenlin@163.com

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摘要：

背景：育亨宾、纳洛酮作为临床常用麻醉拮抗剂，尤其是在拮抗氯胺酮麻醉方面运用广泛。
目的：观察育亨宾、纳洛酮及二者合用对氯胺酮麻醉小鼠的催醒作用。
方法：昆明种小鼠40只随机分成4组，所有小鼠均腹腔注射氯胺酮制作麻醉模型，待翻正反射消失1 min后，不同实验组分别采用腹腔注射生理盐水、育亨宾、纳洛酮、育亨宾+纳洛酮处理，观察翻正反射消失的持续时间(恢复时间)。
结果与结论：与生理盐水处理组相比，育亨宾组、纳洛酮组、育亨宾+纳洛酮组小鼠的睡眠时间均明显缩短，两药合用组比单独用药组睡眠缩短时间明显。提示育亨宾与纳洛酮及二者合用能有效拮抗氯胺酮的麻醉效应。

关键词: 氯胺酮, 育亨宾, 纳洛酮, 催醒作用, 麻醉模型, 小鼠

Abstract:

BACKGROUND: Yohimbine and naloxone are widely used as narcotic antagonists in clinic, especially against ketamine-induced anesthesia.
OBJECTIVE: To investigate the analeptic effects of yohimbine, naloxone and their combination on ketamine-induced anesthesia in mice.
METHODS: Forty Kunming mice were randomly assigned to four groups, and all mice were intraperitoneally administered with ketamine. One minute after loss of righting reflex, different groups were intraperitoneally injected with normal saline, yohimbine, naloxone, yohimbine+naloxone, respectively. The duration of loss righting reflex (sleeping time) was recorded.
RESULTS AND CONCLUSION: Compared with the normal saline group, the sleeping time in the yohimbine, naloxone and yohimbine+naloxone groups was obviously decreased. In addition, significant difference was also observed between the single treated and combined groups. Yohimbine, naloxone and their combination can efficaciously play an antagonistic role in the narcotism of ketamine.

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