中国组织工程研究

中国组织工程研究 ›› 2021, Vol. 25 ›› Issue (16): 2554-2561.doi: 10.3969/j.issn.2095-4344.3118

• 材料生物相容性 material biocompatibility • 上一篇    下一篇

丝素蛋白/姜黄素复合膜敷料促进皮肤创面愈合的评价

陈珍玉，张小宁，罗钰昕，梁健威，晏  驰   

  1. 西南大学家蚕基因组生物学国家重点实验室，西南大学生物技术学院，重庆市   400715
  • 收稿日期:2020-06-28 修回日期:2020-07-02 接受日期:2020-08-04 出版日期:2021-06-08 发布日期:2021-01-07
  • 通讯作者: 张小宁，博士，副教授，西南大学生物技术学院，重庆市 400715
  • 作者简介:陈珍玉，女，1996年生，重庆市人，汉族，西南大学在读硕士，主要从事蚕丝蛋白在生物医用材料领域的应用研究工作。
  • 基金资助:
    重庆市茧丝绸发展补助资金(CQ2019JSCC03)，项目负责人：张小宁；重庆市留学人员回国创业创新支持计划(cx2019098)，项目负责人：张小宁；2020年西南大学国家级大学生创新创业训练计划项目资助(202010635088)，项目负责人：晏驰

Evaluation of silk fibroin/curcumin composite film for promoting wound healing

Chen Zhenyu, Zhang Xiaoning, Luo Yuxin, Liang Jianwei, Yan Chi   

  1. State Key Laboratory of Silkworm Genome Biology, College of Biotechnology, Southwest University, Chongqing 400715, China
  • Received:2020-06-28 Revised:2020-07-02 Accepted:2020-08-04 Online:2021-06-08 Published:2021-01-07
  • Contact: Zhang Xiaoning, PhD, Associate Professor, State Key Laboratory of Silkworm Genome Biology, College of Biotechnology, Southwest University, Chongqing 400715, China
  • About author:Chen Zhenyu, Master candidate, State Key Laboratory of Silkworm Genome Biology, College of Biotechnology, Southwest University, Chongqing 400715, China
  • Supported by:
    the Subsidy Funds for the Development of Silk Industry Sponsored by Chongqing Municipal Commission of Commerce, No. CQ2019JSCC03 (to ZXN); Venture & Innovation Support Program for Chongqing Overseas Returnees, No. cx2019098 (to ZXN); National Training Program of Innovation and Entrepreneurship for Undergraduates in Southwest University in 2020, No. 202010635088 (to YC)

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摘要：

文题释义：
皮肤创面愈合：在致伤因子作用下皮肤等组织出现损伤后的一系列愈合过程，创面愈合过程一般分为炎症反应、肉芽组织形成、再上皮化及伤口闭合后塑形几个阶段。伤口愈合过程中应当注意不被细菌感染，因为伤口感染会增加患者的痛苦或导致并发症，进而延长伤口愈合时间。
姜黄素光敏化：姜黄素具有良好的抗菌效果，在光照条件下姜黄素的抗菌活性增强，这是由于姜黄素在光照下能够产生活性氧，通过氧化损伤机制杀灭细菌。除活性氧外，姜黄素在光照下还会产生姜黄素自由基，可引起明显的DNA损伤。

背景：前期研究成功制备出了丝素蛋白/姜黄素复合膜，经测试其具有良好的理化性质、缓释性能、抑菌性能及细胞相容性等。
目的：进一步观察丝素蛋白/姜黄素复合膜作为敷料修复皮肤创伤的效果。
方法：参照前期研究方法制备丝素蛋白/姜黄素复合膜，检测其力学性能、透光率。以大肠杆菌与金黄色葡萄球菌为菌种，利用平板菌落计数法检测光照后丝素蛋白/姜黄素复合膜、未光照丝素蛋白/姜黄素复合膜与未光照丝素蛋白膜的抑菌性能。在20只昆明小鼠背部制作全层皮肤缺损创面，其中10只覆盖丝素蛋白/姜黄素复合膜，另10只覆盖无菌纱布，术后3，7，14 d，观察创面愈合情况与创面组织学形态。实验方案经西南大学实验动物伦理审查委员会批准(批准号：IACUC-2019011)。
结果与结论：①丝素蛋白/姜黄素复合膜的断裂强度高于丝素蛋白膜(P < 0.05)，杨氏模量显著低于丝素蛋白膜(P < 0.01)；②丝素蛋白膜与丝素蛋白/姜黄素复合膜均具有良好的透光率，但丝素蛋白/姜黄素复合膜在400-450 nm的可见光区域有较强的吸收；③与未光照丝素蛋白膜相比，未光照丝素蛋白/姜黄素复合膜具有较强的抑菌性能；与未光照丝素蛋白/姜黄素复合膜相比，光照后丝素蛋白/姜黄素复合膜具有更强的抑菌性能；④实验组各时间点的创面愈合率高于对照组(P < 0.05)；组织形态学分析显示，实验组第3，7天的再生上皮长度、肉芽组织厚度大于对照组(P < 0.01)，第14天的肉芽组织厚度小于对照组(P < 0.01)；⑤结果表明，丝素蛋白/姜黄素复合膜可促进皮肤缺损创面的愈合。

关键词: 材料, 丝素蛋白膜, 透光性, 姜黄素, 光敏化, 抑菌性, 医用敷料, 昆明小鼠, 创面愈合

Abstract: BACKGROUND: In the previous study, we have successfully prepared a silk fibroin/curcumin composite film, which possesses good physicochemical properties, sustained-release properties, antibacterial activity and biocompatibility.
OBJECTIVE: To further observe the effect of the prepared silk fibroin/curcumin composite film, as the wound dressing, in the field of wound healing.
METHODS: The silk fibroin/curcumin composite film was prepared by referring to the previously established methods. The mechanical properties and light transmittance of the prepared composite film were then investigated. Escherichia coli and Staphylococcus aureus were used as strains. The plate-counting technique was applied to evaluate the antibacterial activity of the light illuminated silk fibroin/curcumin composite film, silk fibroin/curcumin composite film without light exposure, and silk fibroin film without light exposure. The full-thickness excisional lesions were created on the dorsum of 20 Kunming mice. The wounds of 10 mice were covered with silk fibroin/curcumin composite film, and the wounds of other 10 mice were dressed with sterile gauze. The healing status and histological morphology of the wound sites were observed at 3, 7, and 14 days post operation. The animal experimental design and the protocols were approved by the Laboratory Animal Ethics Committee of Southwest University (approval number: IACUC-2019011).
RESULTS AND CONCLUSION: (1) Compared to silk fibroin film, the fracture strength of silk fibroin/curcumin composite film increased significantly (P < 0.05), while the Young’s modulus of silk fibroin/curcumin composite film decreased significantly (P < 0.01). (2) Both silk fibroin film and the silk fibroin/curcumin composite film exhibited excellent transparency, but the silk fibroin/curcumin composite film showed a stronger absorption in visible light region at a wavelength of 400-450 nm. (3) Without light illumination, the silk fibroin/curcumin composite film exhibited stronger antibacterial activity than that of silk fibroin film. While, the silk fibroin/curcumin composite film with light illumination demonstrated stronger antibacterial activity than that of silk fibroin/curcumin composite film without light illumination. (4) The wound healing rate of the experimental group was higher than that of the control group at each time point (P < 0.05). Analysis on histological morphology reveals that the length of regenerated epithelia of dermal tissue and the thickness of granulation tissue in the experimental group were greater than those in the control group at 3 and 7 days post operation (P < 0.01). While, the thickness of granulation tissue was smaller than that in the control group at 14 days post operation (P < 0.01). (5) The results indicated that the silk fibroin/curcumin composite film can facilitate skin wound healing.


Key words: material, silk fibroin film, optical transmission, curcumin, photosensitization, antibacterial activity, wound dressing, Kunming mice, wound healing

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