中国组织工程研究 ›› 2019, Vol. 23 ›› Issue (9): 1319-1323.doi: 10.3969/j.issn.2095-4344.1642

• 骨髓干细胞 bone marrow stem cells • 上一篇    下一篇

CXCL12和CXCR4对骨髓干细胞向子宫内膜间质细胞趋化的影响

黄晓萍,张 娟,王文平,范 波   

  1. 川北医学院附属医院,四川省南充市 637000
  • 修回日期:2018-11-30 出版日期:2019-03-28 发布日期:2019-03-28
  • 通讯作者: 黄晓萍,副主任医师,川北医学院附属医院,四川省南充市 637000
  • 作者简介:黄晓萍,女,1966年生,四川省广安市人,汉族,1990年泸州医学院毕业,副主任医师,主要从事产科方面的研究。
  • 基金资助:

    四川省医学科研课题计划(S15026),项目负责人:黄晓萍

Effects of CXCL12 and CXCR4 on chemotaxis of bone marrow stem cells towards endometrial stromal cells

Huang Xiaoping, Zhang Juan, Wang Wenping, Fan Bo   

  1. Affiliated Hospital of North Sichuan Medical College, Nanchong 637000, Sichuan Province, China
  • Revised:2018-11-30 Online:2019-03-28 Published:2019-03-28
  • Contact: Huang Xiaoping, Affiliated Hospital of North Sichuan Medical College, Nanchong 637000, Sichuan Province, China
  • About author:Huang Xiaoping, Associate chief physician, Affiliated Hospital of North Sichuan Medical College, Nanchong 637000, Sichuan Province, China
  • Supported by:

    the Medical Research Project of Sichuan Province, No. S15026 (to HXP)

摘要:

文章快速阅读:

文题释义:
趋化因子受体CXCR4:
在体内大部分组织和器官上都有表达,它是由352个氨基酸组成的G蛋白偶联受体,具有7次穿膜结构。CXCR4参与体内多种生理机制,包括参与HIV-1病毒侵染、造血功能、胚胎发育及肿瘤迁移等。
趋化因子CXCL12:是一种具有趋化活性的细胞因子,也被称为基质细胞衍生因子1,最早从骨髓基质细胞中分离获得。CXCR4是CXCL12的特异性受体,CXCL12及其受体CXCR4构成的生物轴在介导造血干细胞迁移和归巢、炎症反应、恶性肿瘤的浸润转移等方面有重要作用。

 

摘要
背景:
骨髓干细胞可定植于子宫内膜损伤小鼠的子宫内膜组织,然而促进骨髓干细胞向子宫内膜迁移的分子机制目前尚不清楚。
目的:探讨CXCL12和CXCR4对骨髓干细胞向子宫内膜间质细胞趋化的影响。
方法:将小鼠骨髓干细胞和人子宫内膜间质细胞进行血清饥饿过夜,用雌二醇处理24 h,RT-PCR检测小鼠骨髓干细胞中CXCR4的mRNA表达,ELISA检测小鼠骨髓干细胞和人子宫内膜间质细胞中CXCL12水平,Transwell小室观察小鼠骨髓干细胞向人子宫内膜间质细胞的迁移情况。
结果与结论:雌二醇预处理可增加人子宫内膜间质细胞中CXCL12的表达和小鼠骨髓干细胞中CXCR4的表达,促进小鼠骨髓干细胞迁移到人子宫内膜间质细胞条件培养基,结果表明,雌二醇能通过CXCL12/CXCR4信号通路促进骨髓干细胞迁移到子宫内膜,为子宫内膜疾病的治疗提供了新思路。


中国组织工程研究杂志出版内容重点:干细胞;骨髓干细胞;造血干细胞;脂肪干细胞;肿瘤干细胞;胚胎干细胞;脐带脐血干细胞;干细胞诱导;干细胞分化;组织工程
ORCID: 0000-0001-7670-9954(黄晓萍)

关键词: 骨髓干细胞, 子宫内膜间质细胞, CXCL12, CXCR4, 雌二醇

Abstract:

BACKGROUND: Bone marrow stem cells can be colonized into the mouse endometrium. However, the molecular mechanisms underlying the migration of bone marrow stem cells into the endometrium are still unclear.
OBJECTIVE: To investigate the effects of CXCL12 and CXCR4 on the chemotaxis of bone marrow stem cells towards endometrial stromal cells.
METHODS: Mouse bone marrow stem cells and human endometrial stromal cells were serum-starved overnight. After 24 hours of estradiol pretreatment, the mRNA expressions of CXCR4 and CXCL12 in the mouse bone marrow stem cells were detected by RT-PCR; the levels of CXCL12 in the mouse bone marrow stem cells and human endometrial stromal cells were detected by ELISA; and the migration of mouse bone marrow stem cells into human endometrial stromal cells was observed by Transwell chamber test.
RESULTS AND CONCLUSION: Estradiol pretreatment could upregulate the expression of CXCL12 in the human endometrial stromal cells as well as the expression of CXCR4 in the mouse bone marrow cells, promoting the migration of mouse bone marrow stem cells towards the conditioned medium of human endometrial stromal cells. In conclusion, estradiol can promote the migration of bone marrow stem cells into the endometrium, which may provide new ideas for the treatment of endometrial diseases.


中国组织工程研究杂志出版内容重点:干细胞;骨髓干细胞;造血干细胞;脂肪干细胞;肿瘤干细胞;胚胎干细胞;脐带脐血干细胞;干细胞诱导;干细胞分化;组织工程

Key words: Bone Marrow, Stem Cells, Endometrium, Estradiol, Tissue Engineering

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