中国组织工程研究

中国组织工程研究 ›› 2018, Vol. 22 ›› Issue (36): 5760-5765.doi: 10.3969/j.issn.2095-4344.0543

• 骨组织构建 bone tissue construction • 上一篇    下一篇

早期膝骨关节炎患者血清雌二醇和睾酮水平与病变程度的关系

黄  捷1，薄占东2，刘  刚3，罗世兴4   

  1. (1广西医科大学第二附属医院，广西壮族自治区南宁市  530007；2广西医科大学第一附属医院，广西壮族自治区南宁市  530021；3广西医科大学第四附属医院，广西壮族自治区柳州市  545005；4广西医科大学第九附属医院，广西壮族自治区北海市  536000)
  • 收稿日期:2018-04-02 出版日期:2018-12-28 发布日期:2018-12-28
  • 通讯作者: 罗世兴，教授，主任医师，广西医科大学第九附属医院，广西壮族自治区北海市 536000
  • 作者简介:黄捷，男，1990年生，广西壮族自治区贵港市人，汉族，2017年广西医科大学毕业，硕士，医师，主要从事骨科方向研究。
  • 基金资助:

    国家自然科学基金(81460345)

Correlation between serum estradiol and testosterone levels and severity of early-stage knee osteoarthritis in patients

Huang Jie1, Bo Zhandong2, Liu Gang3, Luo Shixing4   

  1.  (1the Second Affiliated Hospital of Guangxi Medical University, Nanning 530007, Guangxi Zhuang Autonomous Region, China; 2the First Affiliated Hospital of Guangxi Medical University, Nanning 530021, Guangxi Zhuang Autonomous Region, China; 3Fourth Affiliated Hospital of Guangxi Medical University, Liuzhou 545005, Guangxi Zhuang Autonomous Region, China; 4the Ninth Affiliated Hospital of Guangxi Medical University, Beihai 536000, Guangxi Zhuang Autonomous Region, China)
  • Received:2018-04-02 Online:2018-12-28 Published:2018-12-28
  • Contact: Luo Shixing, Professor, Chief physician, the Ninth Affiliated Hospital of Guangxi Medical University, Beihai 536000, Guangxi Zhuang Autonomous Region, China
  • About author:Huang Jie, Master, Physician, the Second Affiliated Hospital of Guangxi Medical University, Nanning 530007, Guangxi Zhuang Autonomous Region, China
  • Supported by:

    the National Natural Science Foundation of China, No. 81460345

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摘要：

文章快速阅读：

文题释义：
电化学发光法：是一种在电极表面由电化学引发的特异性发光反应，包括电化学和化学发光两个部分。分析中应用的标记物为电化学发光的底物三联吡啶钌或其衍生物N-羟基琥珀酰胺(NHS)酯，可通过化学反应与抗体或不同化学结构抗原分子结合，制成标记的抗体或抗原。其基本原理是发光底物二价的三联吡啶钌及反应参与物三丙胺在电极表面失去电子而被氧化。氧化的三丙胺失去一个H+而成为强还原剂，将氧化型的三价钌还原为激发的二价钌，随即释放光子而恢复为基态的发光底物。这一过程在电极表面周而复始地进行，不断地发出光子而常保持底物浓度的恒定。
糖胺聚糖(glycosaminoglycan)：曾称黏多糖(mucopolysaccharide)、氨基多糖和酸性多糖，为杂多糖的一种，主要存在于高等动物结缔组织中，植物中也有发现。研究证明糖胺聚糖具有保护关节软骨退化的作用。
摘要
背景：研究表明膝关节骨关节炎绝经后女性的患病率更明显高于同龄段男性，根据膝关节骨关节炎特殊的发病年龄和性别提示性激素水平与膝关节骨关节炎的发病机制可能有密切相关性。
目的：在早期的膝关节骨关节炎患者中，探索绝经后女性和同龄男性的血清中性激素水平与膝关节骨关节炎严重程度的相关性。
方法：采集膝关节骨关节炎患者44例和正常人29例，共73例，按性别差异分为绝经后女性组(n=37)和对应年龄段男性组(n=36)，两组再参照Kellgren-Lawrence(K-L)分级标准，分为K-L 0级、Ⅰ级、Ⅱ级3个亚组。采用电化学发光法检测各组血清中的雌二醇和睾酮水平，分别关联受试者的K-L分级，分析其与K-L分级的相关性。
结果与结论：①在绝经后女性膝关节骨关节炎患者中，K-L分级0级与Ⅰ级者血清中雌二醇的水平差异无显著性意义(P > 0.05)，0级、Ⅰ级分别与Ⅱ级的血清中雌二醇水平差异有显著意义(P < 0.05)；②绝经后女性膝关节骨关节炎按K-L分级0级、Ⅰ级、Ⅱ级的血清中睾酮水平两两比较差异均无显著性意义(P > 0.05)；③绝经后女性膝关节骨关节炎严重程度与血清雌二醇水平呈现出明显的负相关性(rs=-0.709, P < 0.05)，与血清睾酮水平无明显相关性(P > 0.05)；④在对应年龄段男性膝关节骨关节炎患者中，K-L分级0级与Ⅰ级的血清中雌二醇水平和睾酮差异均无显著意义(P > 0.05)，0级、Ⅰ级分别与Ⅱ级的血清中雌二醇和睾酮水平差异均有显著意义(P < 0.05)；⑤对应年龄段男性膝关节骨关节炎严重程度与血清雌二醇水平(rs=-0.476，P < 0.05)和睾酮水平(rs=-0.418，P < 0.05)均呈现出明显的负相关性；⑥结果提示，绝经后女性早期膝关节骨关节炎严重程度与血清中雌二醇水平存在负相关；对应年龄段的男性早期膝关节骨关节炎严重程度与血清中雌二醇和睾酮水平均存在负相关。

中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程
ORCID: 0000-0003-2728-3402(黄捷)

关键词: 膝骨关节炎, 早期, 血清, 性激素, 男性, 女性, 雌二醇, 睾酮, K-L分级, 国家自然科学基金

Abstract:

BACKGROUND: The incidence of knee osteoarthritis (KOA) in postmenopausal females has been found to be higher than that in the males of the same age. The age and gender distribution suggests that there exists certain correlation between KOA pathogenesis and sex hormone levels.
OBJECTIVE: To explore the correlation between sex hormone levels of the postmenopausal females and males of the same age, and early-stage KOA severity.
METHODS: Forty-four KOA patients and 29 healthy people were enrolled. The subjects were divided into two groups by sex, including postmenopausal female group (n=37) and male group age (n=36). Each group was further subdivided by Kellgren-Lawrence K-L grading system, including subgroups of K-L 0, K-L I and K-L II. The serum estradiol and testosterone levels in each subgroup were detected by electrochemical luminescence method, and its association with K-L grade was analyzed.
RESULTS AND CONCLUSION: In the postmenopausal female KOA patients, the serum estradiol level showed no significant difference between K-L 0 and K-L I subgroups (P > 0.05), the level in the K-L 0 and K-L I subgroups showed significant difference compared with the K-L II subgroup (P < 0.05). The serum testosterone level showed no significant difference among groups in the postmenopausal female KOA patients (P > 0.05). The KOA severity showed a negative correlation with the serum estradiol level (rs=-0.709, P < 0.05) but no correlation with the serum testosterone (P > 0.05). In the male KOA patients, the serum estradiol and testosterone levels showed no significant difference between K-L 0 and K-L I subgroups (P > 0.05), the levels in the K-L 0 and K-L I subgroups showed significant difference compared with the K-L II subgroup (P < 0.05). The KOA severity in male KOA patients showed a negative correlation with the serum estradiol level (rs=-0.476,  P < 0.05) and serum testosterone level (rs=-0.418, P < 0.05). These results indicate that the severity of early-stage KOA shows a negative correlation with the serum estradiol level in the postmenopausal female patients and a negative correlation with the serum estradiol and testosterone levels in the males of the same age.

中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程

Key words: Osteoarthritis, Knee, Estradiol, Testosterone, Tissue Engineering

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