中国组织工程研究

中国组织工程研究 ›› 2019, Vol. 23 ›› Issue (6): 951-956.doi: 10.3969/j.issn.2095-4344.1540

• 材料力学及表面改性 material mechanics and surface modification • 上一篇    下一篇

Triton-x100与丹参酚酸B制备脱细胞血管支架及其生物力学性能

赵 亮1，2，李霞飞3，周 坤2，闫欢欢2，张其清2   

  1. 1北京大学工学院，北京市 100871；新乡医学院，2生命科学技术学院，生命科学与健康研究院，3生物医学工程学院，河南省新乡市 453003
  • 收稿日期:2018-10-25 出版日期:2019-02-28 发布日期:2019-02-28
  • 通讯作者: 赵亮，北京大学工学院，北京市 100871；新乡医学院生命科学技术学院，生命科学与健康研究院，河南省新乡市 453003
  • 作者简介:赵亮，男，河南省新乡市人，博士，主要从事组织工程和人工血管研究。
  • 基金资助:

    河南省高等学校重点科研项目计划(17A180034)，项目负责人：赵亮；第二届大学生生命科学联赛立项课题(SKYLS017)，项目负责人：赵亮

Preparation of acellular vascular scaffold using Triton-x100 and salvianolic acid B and its biomechanical performance

Zhao Liang1, 2, Li Xiafei3, Zhou Kun2, Yan Huanhuan2, Zhang Qiqing2   

  1. 1College of Engineering, Peking University, Beijing 100871, China; 2School of Life Sciences and Technology, Life Science and Health Research Institute, Xinxiang Medical University, Xinxiang 453003, Henan Province, China; 3School of Biomedical Engineering, Xinxiang Medical University, Xinxiang 453003, Henan Province, China
  • Received:2018-10-25 Online:2019-02-28 Published:2019-02-28
  • Contact: Zhao Liang, College of Engineering, Peking University, Beijing 100871, China; School of life Sciences and Technology, Life Science and Health Research Institute, Xinxiang Medical University, Xinxiang 453003, Henan Province, China
  • About author:Zhao Liang, PhD, College of Engineering, Peking University, Beijing 100871, China; School of Life Sciences and Technology, Life Science and Health Research Institute, Xinxiang Medical University, Xinxiang 453003, Henan Province, China
  • Supported by:

    The Key Scientific Research Project of Henan Higher Education Institutions, No. 17A180034 (to ZL); the Project of the Second College Student Life Sciences League, No. SKYLS017 (to ZL)

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摘要：

文章快速阅读：

 

文题释义：
脱细胞血管支架：是极佳的血管移植材料，在制备过程中将原材料彻底去除细胞成分，最大限度地保存血管壁成分中的弹性纤维和胶原纤维，最大程度地减低移植产生的免疫原性，较为完好地保留种子细胞黏附生长所需的三维微环境，以及机械性能良好等优点，因此在血管组织工程中得到了越来越多的重视。
细胞-材料复合物的制备：从机体获取少量的活体组织，用特殊的酶或其他方法将细胞(又称种子细胞)从组织中分离出来在体外进行培养扩增，然后将扩增的细胞与具有良好生物相容性、可降解性和可吸收的生物材料(支架)按一定的比例混合，使细胞黏附在生物材料(支架)上形成细胞-材料复合物。
 
 
背景：脱细胞血管支架具有优良性能，但是在其制备过程中其生物力学性能出现不同程度的降低。
目的：观察在组织工程制备脱细胞血管支架中丹参酚酸B的应用前景。
方法：经Triton-x100单独处理，Triton-x100-丹参酚酸B联合处理制备脱细胞血管支架，分别设为Tx组和Tx-sal组，对其进行形态学观察、组织厚度测量、孔隙率检测及生物力学特性检测，比较在应用丹参酚酸B后处理之后脱细胞血管支架在形态学等各种指标上的变化。

结果与结论：Tx组和Tx-sal组的血管管壁厚度及血管孔隙率差异无显著性意义(P > 0.05)，形态学无明显变化。Tx-sal组的脱细胞血管支架可以较完好地保留血管壁的胶原纤维和弹性纤维，抗拉强度、最大变形量、断裂伸长比、爆裂压力及缝合强度均优于Tx组(P < 0.05)。结果证实，Triton-x100联合丹参酚酸B处理能够成功制备脱细胞效果良好、生物力学性能优良的脱细胞血管支架。

ORCID: 0000-0001-8264-2944(赵亮)

中国组织工程研究杂志出版内容重点：生物材料；骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性；组织工程

关键词: 丹参酚酸B, 脱细胞血管支架, 血管移植材料, 孔隙率, 抗拉强度, 主动脉, 细胞-材料复合物, 缝合强度, 组织构建

Abstract:

BACKGROUND: The acellular vascular scaffold has excellent properties, but its biomechanical properties have been reduced to varying degrees during preparation.

OBJECTIVE: To explore the application prospect of salvianolic acid B in the tissue-engineered preparation of acellular vascular scaffold.
METHODS: The rat thoracic aorta was decellularized using Triton-x100 alone (Tx group) or combined with salvianolic acid B (Tx-sal group) to prepare acellular vascular scaffolds. Morphology of the two groups of acellular vascular scaffolds was observed. Measurements of tissue thickness, porosity and biomechanical properties were conducted, and changes in the morphology of acellular vascular scaffolds were compared between two groups.
RESULTS AND CONCLUSION: The use of salvianolic acid B treatment had no effect on the wall thickness and porosity of acellular vascular scaffolds, and there was no significant difference between the Tx group and Tx-sal group (P > 0.05). There was also no significant change in the scaffold morphology. Compared with the Tx group, the acellular vascular scaffold in the Tx-sal group was better to retain collagen fibers and elastic fibers of the vessel wall. The biomechanical indicators in the Tx-sal gorup such as tensile strength, maximum deformation, elongation at break, burst pressure, and suture strength were all superior to those in the Tx group (P < 0.05). Therefore, Triton-x100 combined with salvianolic acid B treatment can produce acellular vascular materials with good decellularization and excellent biomechanical properties. 

Key words: Stents, Biomechanics, Tissue Engineering

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