中国组织工程研究

中国组织工程研究 ›› 2019, Vol. 23 ›› Issue (22): 3556-3561.doi: 10.3969/j.issn.2095-4344.1282

• 材料生物相容性 material biocompatibility • 上一篇    下一篇

Ⅱ型胶原酶对兔角膜生物力学特性的影响

陈昕妍，秦  晓，张海霞，李  林
  

  1. 首都医科大学生物医学工程学院，首都医科大学临床生物力学应用基础研究北京市重点实验室，北京市  100069
  • 收稿日期:2019-03-20
  • 通讯作者: 李林，博士，教授，博士生导师，首都医科大学生物医学工程学院，首都医科大学临床生物力学应用基础研究北京市重点实验室，北京市 100069
  • 作者简介:陈昕妍，女，1997年生，福建省人，汉族，首都医科大学在读硕士，主要从事眼生物力学方面的研究。
  • 基金资助:

    国家自然科学基金项目(31470914)，项目负责人：张海霞；国家自然科学基金项目(31370952)，项目负责人：李林

Effect of collagenase type II on the biomechanical properties of rabbit cornea

Chen Xinyan, Qin Xiao, Zhang Haixia, Li Lin
  

  1. School of Biomedical Engineering, Capital Medical University, Beijing Key Laboratory of Fundamental Research on Biomechanics in Clinical Application, Capital Medical University, Beijing 100069, China
  • Received:2019-03-20
  • Contact: Li Lin, MD, Professor, Doctoral supervisor, School of Biomedical Engineering, Capital Medical University, Beijing Key Laboratory of Fundamental Research on Biomechanics in Clinical Application, Capital Medical University, Beijing 100069, China
  • About author:Chen Xinyan, Master candidate, School of Biomedical Engineering, Capital Medical University, Beijing Key Laboratory of Fundamental Research on Biomechanics in Clinical Application, Capital Medical University, Beijing 100069, China
  • Supported by:

    the National Natural Science Foundation of China, Nos. 31470914 (to ZHX), 31370952 (to LL)

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摘要：

文章快速阅读：

 

文题释义：
Ⅱ型胶原酶：也称基质金属蛋白酶8，是一种只作用于胶原或胶原变形水解产物明胶，而不作用于其他蛋白的酶类。Ⅱ型胶原酶可通过切断胶原分子的多肽链而水解Ⅰ型胶原、Ⅱ型胶原和Ⅲ型胶原，从而导致细胞外基质的变性。
角膜生物力学特性：角膜是人眼的主要屈光介质，同许多其他生物软组织一样，具有非线性、黏弹性和各向异性的复杂生物力学特性。角膜的生物力学特性对维持角膜正常形态及功能具有重要作用，对角膜生物力学特性的检测有利于疾病的诊断与治疗。
 
 
背景：Ⅱ型胶原酶的异常表达与许多角膜的病理状态相关，其作用导致的角膜基质降解可能引起角膜力学性质的改变，而角膜的力学特性又与角膜形态维持、屈光状态密切相关。
目的：观察Ⅱ型胶原酶作用后兔角膜生物力学性质的变化。
方法：6只实验兔麻醉后左眼角膜去上皮，滴加Ⅱ型胶原酶溶液(18 g/L，200 μL)静置30 min。正常饲养3个月。实验经首都医科大学动物实验及实验动物福利委员会的批准，伦理批号：AEEI-2014-066。
结果与结论：与对照眼相比，实验眼在低、高应变区的弹性模量分别减少51.5%和29.9%，总体上实验眼的松弛程度小于对照眼。提示Ⅱ型胶原酶作用后角膜力学特性有所改变，弹性模量降低，应力松弛程度总体上减少，角膜整体承载能力下降。

关键词: Ⅱ型胶原蛋白酶, 角膜, 生物力学特性, 单轴拉伸, 弹性模量, 应力松弛, 应力, 应变

Abstract:

BACKGROUND: Many pathological states of the cornea are associated with abnormal expression of collagenase type II, which can cause corneal matrix degradation and may result in the changes of corneal mechanical properties. The mechanical properties of the cornea are closely related to the corneal shape and refractive state.
OBJECTIVE: To observe the changes in the mechanical properties of the rabbit cornea after treatment with collagenase type II.
METHODS: After anesthesia and epithelial debridement, a collagenase type II solution (18 g/L, 200 μL) was used to treat the left eyes of the experimental rabbits for 30 minutes. Then all rabbits were normally raised for 3 months. The experiments were approved by the Ethics Committee of Animal Experiment and Laboratory Animal Welfare of Capital Medical University (approval No. AEEI-2014-066).  
RESULTS AND CONCLUSION: Compared with the control eyes, the elastic modulus of the experimental eyes in the low and high strain regions decreased by 51.5% and 29.9%, respectively. Overall, the degree of stress relaxation of the experimental eyes was smaller than that of the control eyes. These results suggest that collagenase type II eads to changes in corneal mechanical properties, decreases in elastic modulus, decreases in the degree of overall stress relaxation, and decreases in the overall carrying capacity of the cornea.  

Key words: collagenase type Ⅱ, cornea, mechanical properties, uniaxial stretching, elastic modulus, stress relaxation, stress, strain

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