中国组织工程研究

中国组织工程研究 ›› 2018, Vol. 22 ›› Issue (25): 4077-4082.doi: 10.3969/j.issn.2095-4344.0924

• 干细胞基础实验 basic experiments of stem cells • 上一篇    下一篇

白细胞介素1β与机械牵拉共同作用角膜成纤维细胞CollagenⅠ及Lumican的表达

冯鹏飞，李晓娜，容 烁，陈维毅，王晓君   

  1. 太原理工大学应用力学与生物医学工程研究所，山西省材料强度与结构冲击重点实验室，山西省太原市 030024
  • 修回日期:2018-04-21 出版日期:2018-09-08 发布日期:2018-09-08
  • 通讯作者: 陈维毅，博士生导师，太原理工大学应用力学与生物医学工程研究所，山西省材料强度与结构冲击重点实验室，山西省太原市 030024
  • 作者简介:冯鹏飞，男，1985年生，汉族，山西省运城市人。
  • 基金资助:

    国家自然科学基金项目(11572213，31271005，11402161，11402162)；山西省研究生教育创新项目(2015BY28)

Combined effects of interleukin 1beta and mechanical stretching on Collagen I synthesis and Lumican expression in corneal fibroblasts

Feng Peng-fei, Li Xiao-na, Rong Shuo, Chen Wei-yi, Wang Xiao-jun   

  1. Key Laboratory of Material Intensity and Structural Impact in Shanxi Province, Institute of Applied Mechanics and Biomedical Engineering, Taiyuan University of Technology, Taiyuan 030024, Shanxi Province, China
  • Revised:2018-04-21 Online:2018-09-08 Published:2018-09-08
  • Contact: Chen Wei-yi, Doctoral supervisor, Key Laboratory of Material Intensity and Structural Impact in Shanxi Province, Institute of Applied Mechanics and Biomedical Engineering, Taiyuan University of Technology, Taiyuan 030024, Shanxi Province, China
  • About author:Feng Peng-fei, Key Laboratory of Material Intensity and Structural Impact in Shanxi Province, Institute of Applied Mechanics and Biomedical Engineering, Taiyuan University of Technology, Taiyuan 030024, Shanxi Province, China
  • Supported by:

    the National Natural Science Foundation of China, No. 11572213, 31271005, 11402161, 11402162; the Graduate Student Education Innovation Project in Shanxi Province, No. 2015BY28

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摘要：

文章快速阅读：

文题释义：
机械牵拉：是体外研究组织细胞对力学刺激响应的一种技术，可体外模拟细胞在组织中的受力情况，研究细胞的力学生物学效应。细胞可以通过力转导信号途径感受力学刺激来改变细胞外基质的结构和功能。
Lumican和白细胞介素1β：Lumican是富含亮氨酸的小分子蛋白，是角膜中主要的蛋白聚糖，可以调节胶原纤维的结构和角膜的透明性。白细胞介素1β是与组织损伤有关的炎性因子，在眼部疾病中起着重要的介导作用，可引起炎症反应、细胞凋亡和细胞外基质的积聚。

 

摘要
背景：继发性圆锥角膜是角膜屈光手术后的并发症之一，但其发生机制尚不清楚。
目的：探讨白细胞介素1β与机械牵拉对角膜成纤维细胞CollagenⅠ和Lumican表达的影响。
方法：体外培养角膜成纤维细胞，对其施加不同幅度(5%，10%，15%)的机械牵拉和不同质量浓度的白细胞介素1β，共同作用12，24，36 h，通过实时荧光定量PCR检测CollagenⅠ和Lumican mRNA的表达变化。
结果与结论：①白细胞介素1β单独作用12 h使CollagenⅠα1的表达降低(P < 0.05)，作用24 h使Lumican的表达升高(P < 0.05)；②5%牵拉单独作用24，36 h使CollagenⅠα1表达升高(P < 0.05)；10%和15%牵拉单独作用12，24，36 h使CollagenⅠα1和CollagenⅠα2的表达降低(P < 0.05)；③5%牵拉单独作用12 h使Lumican表达升高(P < 0.05)，而10%和15%牵拉单独作用12 h使Lumican表达降低(P < 0.05)；5%，10%，15%牵拉24 h和36 h时使Lumican表达升高(P < 0.05)；④白细胞介素1β和机械牵拉共同作用24，36 h使CollagenⅠα1和CollagenⅠα2表达降低，Lumican表达升高；⑤结果提示，白细胞介素1β能抑制CollagenⅠ的合成，而促进Lumican的表达。低幅度机械牵拉能促进角膜成纤维细胞胶原的合成，中高幅度机械牵拉抑制其胶原的合成。两者共同作用能抑制角膜成纤维细胞胶原合成且促进Lumican的表达。

中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程
ORCID: 0000-0003-1702-5653(陈维毅)

关键词: 角膜, 成纤维细胞, 白细胞介素1β, 机械牵拉, CollagenⅠ, Lumican, 国家自然科学基金

Abstract:

BACKGROUND: Secondary keratoconus is one of the complications after corneal refractive surgery, but the mechanism remains unclear.
OBJECTIVE: To study the effects of interleukin-1β and mechanical stretching on the synthesis of the Collagen I and Lumican in corneal fibroblasts. 
METHODS: Rabbit corneal fibroblasts were cultured in vitro and were treated by different concentrations of interleukin-1β and different amplitudes of mechanical stretching (5%, 10%, 15%) for 12, 24 or 36 hours. The expression of Collagen I and Lumican was detected by real-time fluorescent quantitative PCR.
RESULTS AND CONCLUSION: Interleukin-1β decreased the expression of Collagen I α1 at 12 hours and increased the expression of Lumican at 24 hours (P < 0.05). 5% stretching alone increased the expression of Collagen I α1 at 24 and 36 hours (P < 0.05), but 10% and 15% stretching alone decreased the expression of Collagen I α1 and Collagen I α2 at 12, 24 and 36 hours (P < 0.05). 5% stretching alone increased the expression of Lumican at 12 hours (P < 0.05), while 10% and 15% stretching alone decreased the expression of Lumican at 12 hours (P < 0.05). Moreover, 5%, 10% and 15% stretching alone increased the expression of Lumican at 24 and 36 hours (P < 0.05). Reduced expression of Collagen I α1 and Collagen I α2 and increased expression of Lumican in corneal fibroblasts were observed at 24 and 36 hours after combined treatment with interleukin-1β and mechanical stretching. These results reveal that interleukin-1β can inhibit the synthesis of Collagen I and promote the expression of Lumican. Low-amplitude stretching can increase collagen synthesis; however, middle-to-large- amplitude stretching can inhibit its expression in corneal fibroblasts. The synthesis of collagen can be suppressed and the expression of Lumican can be increased under the combined use of interleukin-1β and stretching.

中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程

Key words: Cornea, Fibroblasts, Interleukin-1beta, Collagen Type I, Tissue Engineering

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