中国组织工程研究 ›› 2018, Vol. 22 ›› Issue (20): 3184-3189.doi: 10.3969/j.issn.2095-4344.0273

• 组织构建实验造模 experimental modeling in tissue construction • 上一篇    下一篇

自发性犬骨肉瘤动物模型的免疫组织化学检测

刘  洋1,赵得志1,郗  政1,邓志会2,孙  启1,于  水1   

  1. 1齐齐哈尔医学院附属第二医院骨外科,黑龙江省齐齐哈尔市   161006;         2齐齐哈尔医学院医药科学研究院分子生物学中心,黑龙江省齐齐哈尔市   161000
  • 收稿日期:2018-04-21 出版日期:2018-07-18 发布日期:2018-07-18
  • 作者简介:刘洋,男,1976年生,黑龙江省齐齐哈尔市人,2001年佳木斯医学院毕业,副主任医师,主要从事脊柱、骨关节创伤、骨病,运动医学,骨关节疾病的微创治疗的研究。
  • 基金资助:

    2013年黑龙江省医药卫生一般研究计划(2013KYA155)

Immunohistochemical detection in dog models of spontaneous osteosarcoma

Liu Yang1, Zhao De-zhi1, Xi Zheng1, Deng Zhi-hui2, Sun Qi1, Yu Shui1   

  1. 1Department of Orthopedics, the Second Affiliated Hospital of Qiqihar Medical University, Qiqihar 161006, Heilongjiang Province, China; 2Center for Molecular Biology, Academy of Medical Sciences of Qiqihar Medical University, Qiqihar 161000, Heilongjiang Province, China
  • Received:2018-04-21 Online:2018-07-18 Published:2018-07-18
  • About author:Liu Yang, Associate chief physician, Department of Orthopedics, the Second Affiliated Hospital of Qiqihar Medical University, Qiqihar 161006, Heilongjiang Province, China
  • Supported by:

    the General Research Plan of Heilongjiang Provincial Medical Health in 2013, No. 2013KYA155

摘要:

文章快速阅读:
文题释义:
碱性磷酸酶(alkaline phosphatase):是一种能够将对应底物去磷酸化的酶,即通过水解磷酸单酯将底物分子上的磷酸基团除去,并生成磷酸根离子和自由的羟基,这类底物包括核酸、蛋白、生物碱等。碱性磷酸酶是目前免疫诊断试剂产品最常用的标记酶之一。与辣根过氧化物酶(HRP)相比,碱性磷酸酶用作标记酶的优点是,稳定性高、灵敏度高,缺点是成本高,标记困难。
免疫组织化学检测(IHC):用于检测与人体组织或细胞标本。鉴别形态很相似的疾病或肿瘤,确定组织来源及研究肿瘤组织代谢改变,包括细胞内酶学的变化、糖原的变化、核酸的变化等。
摘要
背景
:由于骨肉瘤基因的复杂性,其分子特征至今还没有被完全理解。对于人类骨肉瘤,高表达碱性磷酸酶、转录因子2(Runx2)和骨形态发生蛋白4,部分微表达肌间线蛋白、S100和神经特异性烯醇化酶(NSE),而犬骨肉瘤肿瘤标记物是否与人类相似,尚不确定。
目的:对作为人类疾病模型的犬类骨肉瘤进行验证,评估对于人类骨肉瘤来说重要的标记物的表达情况。
方法:取59例来自于被证实有原发性骨肉瘤的犬类组织切片,进行免疫组织化学标记,检测碱性磷酸酶、肌间线蛋白、S100、神经特异性烯醇化酶、转录因子2和骨形态发生蛋白4的表达。
结果与结论:①碱性磷酸酶、转录因子2和骨形态发生蛋白4在所有肿瘤中高度表达,而肌间线蛋白、S100和神经特异性烯醇化酶表达情况多变;②研究发现与在人类骨肉瘤中的发现类似,表明犬科动物可用作研究人类疾病的模型。

中国组织工程研究杂志出版内容重点:组织构建;骨细胞;软骨细胞;细胞培养;成纤维细胞;血管内皮细胞;骨质疏松组织工程
ORCID: 0000-0002-6810-8734(刘洋)

关键词: 免疫组织化学, 犬骨肉瘤, 人类骨肉瘤, 组织构建

Abstract:

BACKGROUND: Molecular features of osteosarcoma are not fully understood because of the complexity of osteosarcoma gene. In human osteosarcoma, there are highly expressed alkaline phosphatase, runt-related transcription factor 2 (Runx2) and bone morphogenetic protein 4 (BMP4), as well as slightly expressed desmin, S100 and neuro-specific enolase (NSE). However, whether markers of osteosarcoma are similar to those of human osteosarcoma remains unclear.
OBJECTIVE: To verify the canine osteosarcoma experiments simulating human disease model and to assess the expression of important markers for human osteosarcoma.
METHODS: Fifty-nine tissue sections of the canine osteosarcoma were used for immunohistochemical staining. The expression levels of alkaline phosphatase, desmin, S100, NSE, Runx2 and BMP4 were detected.
RESULTS AND CONCLUSION: Alkaline phosphatase, Runx2 and BMP4 were highly expressed in all tumors, while the expression of desmin, S100 and NSE was variable. The findings are similar to those found in human osteosarcoma, suggesting that canine animals can be used as the models for studying human diseases.

中国组织工程研究杂志出版内容重点:组织构建;骨细胞;软骨细胞;细胞培养;成纤维细胞;血管内皮细胞;骨质疏松组织工程

Key words: Osteosarcoma, Disease Models, Animal, Canidae, Tissue Engineering

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