中国组织工程研究

中国组织工程研究 ›› 2012, Vol. 16 ›› Issue (12): 2115-2119.doi: 10.3969/j.issn.1673-8225.2012.12.006

• 组织工程神经材料 tissue-engineered nerve materials • 上一篇    下一篇

甲壳素神经再生室注入聚乳酸-聚乙醇酸-重组人促红细胞生成素微球促进 缺损周围神经的修复

黄亚洲，陈清汉，任明明   

  1. 郑州大学第二附属医院骨科，河南省郑州市   450014
  • 收稿日期:2011-12-12 修回日期:2012-01-14 出版日期:2012-03-18 发布日期:2012-03-18
  • 通讯作者: 陈清汉，硕士，教授，主任医师，郑州大学第二附属医院骨科，河南省郑州市 450014
  • 作者简介:黄亚洲，男，1982年生，河南省睢县人，汉族，2008年河南科技大学毕业，主要从事骨科周围神经方面的研究。 1105831335@ qq.com

Injection of polylactic acid-polyglycolic acid-recombinant human erythropoietin microspheres into chitin nerve regeneration chamber can promote sciatic nerve regeneration

Huang Ya-zhou, Chen Qing-han, Ren Ming-ming   

  1. Department of Orthopedics, the Second Affiliated Hospital of Zhengzhou University, Zhengzhou  450014, Henan Province, China
  • Received:2011-12-12 Revised:2012-01-14 Online:2012-03-18 Published:2012-03-18
  • Contact: author: Chen Qing-han, Master, Professor, Chief physician, Department of Orthopedics, the Second Affiliated Hospital of Zhengzhou University, Zhengzhou 450014, Henan Province, China
  • About author:Huang Ya-zhou, Department of Orthopedics, the Second Affiliated Hospital of Zhengzhou University, Zhengzhou 450014, Henan Province, China 1105831335@ qq.com

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被引次数

4

摘要：

背景：促红细胞生成素除了具有造血的作用以外, 对神经系统损伤的修复也起着重要作用。
目的：观察聚乳酸-聚乙醇酸-重组人促红细胞生成素微球对大鼠坐骨神经再生的作用。
方法：雌性SD大鼠60只，随机分为3组。制备大鼠双侧坐骨神经缺损模型(1 cm缺损)以及可吸收甲壳素神经再生室。实验组室内注入聚乳酸-聚乙醇酸-重组人促红细胞生成素微球；对照组室内注入聚乳酸-聚乙醇酸微球；空白对照组室内注入等渗生理盐水。
结果与结论：实验组再生神经的传导速度优于对照组及空白对照组，且12周优于6周，差异有显著性意义(P < 0.05)。S-100 免疫组织化学及Loyez氏神经染色法显示：实验组神经纤维数量多于对照组及空白对照组，12周多于6周，差异有显著性意义(P < 0.05)。结果提示聚乳酸-聚乙醇酸-重组人促红细胞生成素微球能够促进实验性坐骨神经缺损的再生和功能的恢复。
关键词：重组人促红细胞生成素；周围神经损伤；神经再生；甲壳素导管；传导速度
doi:10.3969/j.issn.1673-8225.2012.12.006

关键词: 重组人促红细胞生成素, 周围神经损伤, 神经再生, 甲壳素导管, 传导速度

Abstract:

BACKGROUND: Erythropoietin (EPO) plays an important role in hematopoiesis as well as in the repair of nervous system injury.
OBJECTIVE: To observe the effect of polylactic acid-polyglycolic acid-recombinant human erythropoietin microspheres on sciatic nerve regeneration.
METHODS: Sixty female SD rats were randomly divided into three groups, 20 rats in each group. The bilateral sciatic nerve defect model was prepared (1 cm defect) and the absorbable chitin nerve regeneration chamber was prepared. The chambers of the rats in the experimental group were injected with polylactic acid-polyglycolic acid-recombinant human erythropoietin microspheres, chambers of the rats in the control group were injected with polylactic acid-polyglycolic acid microspheres and chambers of the rats in the blank control group were injected with normal saline in the same dose.
RESULTS AND CONCLUSION: The conduction velocity of the regeneration nerve in the experimental group was better than that in the control group and blank control group, and the conduction velocity at the 12th week was better than that at the 6th week. There was significant difference (P < 0.05). S-100 immunohistochemistry and Loyezs neural staining showed that the number of nerve fibers in experimental group was more that in control group and blank control group, and the number of nerve fibers at the 12th week was more than that at the 6th week; the difference was statistically significant (P < 0.05). Polylactic acid-polyglycolic acid-recombinant human erythropoietin microspheres has the effect of promoting sciatic nerve regeneration and functional recovery.

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