中国组织工程研究

中国组织工程研究 ›› 2012, Vol. 16 ›› Issue (6): 994-997.doi: 10.3969/j.issn.1673-8225.2012.06.010

• 干细胞培养与分化 stem cell culture and differentiation • 上一篇    下一篇

BrdU标记法检测不同年龄阶段小鼠神经前体细胞的增殖潜能****★

朱  茜1，赵云鹤1，刘雪芹1，杨武林2，陆  利1   

  1. 1山西医科大学人体解剖学教研室，山西省太原市  030001；2合肥工业大学生物与食品工程学院，安徽省合肥市 230009
  • 收稿日期:2011-08-27 修回日期:2011-10-19 出版日期:2012-02-05 发布日期:2012-02-05
  • 通讯作者: 陆利，博士，副教授，硕士生导师，山西医科大学人体解剖学教研室，山西省太原市 030001 luli7300@126.com
  • 作者简介:朱茜★，女，1984年生，山西省长治市人，汉族，山西医科大学在读硕士，主要从事神经干细胞与衰老的相关研究。 zhuqian-20404@163.com
  • 基金资助:

    国家自然科学基金项目(30973094)，课题名称：蛋白酶体β5 亚单位与神经干细胞衰老进程关系及其调控机制；山西省青年科技研究基金项目(2009021045)，课题名称：26s蛋白酶体对神经干细胞衰老进程的影响；山西医科大学大学生创新基金项目(201007)，课题名称：蛋白酶体抑制剂对骨髓间充质干细胞增殖潜能的影响；国家自然科学基金项目(30800178)，课题名称：利用生物标记/蛋白质组法研究APP基因家族胞内段在神经元细胞中的转录分子作用网络。

Bromodeoxyuridine assay for detecting the proliferation of neural progenitor cells in mice at different ages

Zhu Qian1, Zhao Yun-he1, Liu Xue-qin1, Yang Wu-lin2, Lu Li1   

  1. 1Department of Anatomy, Shanxi Medical University, Taiyuan  030001, Shanxi Province, China; 2School of Biotechnology and Food Engineering, Hefei University of Technology, Hefei  230009, Anhui Province, China
  • Received:2011-08-27 Revised:2011-10-19 Online:2012-02-05 Published:2012-02-05
  • Contact: Lu Li, Doctor, Associate professor, Master’s supervisor, Department of Anatomy, Shanxi Medical University, Taiyuan 030001, Shanxi Province, China luli7300@126.com
  • About author:Zhu Qian★, Studying for master’s degree, Department of Anatomy, Shanxi Medical University, Taiyuan 030001, Shanxi Province, China zhuqian-20404@163.com
  • Supported by:

    the National Natural Science Foundation of China, No. 30973094*; Youth Science and Technology Foundation of Shanxi Province, No. 2009021045*; Innovation Foundation of Shanxi Medical University, No. 201007*; the National Natural Science Foundation of China, No. 30800178*

PDF

378

被引次数

8

摘要：

背景：BrdU是胸腺嘧啶核苷类似物，常用来标记前脑室管膜下区具有增殖潜能的神经前体细胞。
目的：建立不同年龄阶段BrdU标记方法，系统分析不同年龄阶段神经前体细胞增殖特点以及变化规律。
方法：选取胚胎期小鼠、新生小鼠、成年小鼠和老年小鼠各5只，腹腔注射BrdU，观察不同年龄阶段神经前体细胞增殖潜能及不同年龄阶段前脑衰老细胞，探讨微环境对神经前体细胞增殖潜能的影响。
结果与结论：胚胎期小鼠、新生小鼠、成年小鼠和老年小鼠前脑室管膜下区BrdU+细胞分别为(897.20±22.38)，(262.17±26.26)，(76.67±5.63)，(43.8±2.61)个，随年龄增加细胞增殖潜能逐渐降低(P < 0.05)。成年小鼠和老年小鼠脑室周围有大量β-半乳糖苷酶染色阳性衰老细胞，说明随年龄增加脑内β-半乳糖苷酶染色活性增强，衰老细胞逐渐增多，神经前体细胞生存微环境失衡可能与神经前体细胞增殖潜能下降有关。

关键词: 神经前体细胞, 5-溴脱氧尿嘧啶核苷, &beta, -半乳糖苷酶, 增殖, 衰老

Abstract:

BACKGROUND: Bromodeoxyuridine (BrdU) is an analog of thymidine that can be used to analyze the proliferation of neural progenitor cells (NPCs) in the forebrain subventricular zone (SVZ).
OBJECTIVE: To systematically assess the proliferation of NPCs in SVZ of mice at different ages by using the optimal BrdU labeling method.
METHODS: Embryonic (n=5), neonatal (n=5), adult (n=5) and aged mice (n=5) were used in this study. Mice were given intraperitoneal injection of BrdU, the proliferation of NPCs in forebrain cells in rats at different ages was analyzed, and the influence of niche on NPCs proliferation was investigated.
RESULTS AND CONCLUSION: The number of BrdU+ cells in SVZ of embryonic, neonatal, adult and aged mice was (897.20±22.38), (262.17±26.26), (76.67±5.63) and (43.8±2.61), respectively. The proliferation of NPCs was decreased as the age increased (P < 0.05). Meanwhile, SA-β-galactosidase staining was used to detect senescence cells in the forebrain，positive cells were found around ventricle in adult and aged mice. Increased activity of SA-β-gal in the brain indicated that age-related changes may influence the proliferation of NPCs.

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