中国组织工程研究

中国组织工程研究 ›› 2012, Vol. 16 ›› Issue (5): 871-874.doi: 10.3969/j.issn.1673-8225.2012.05.027

• 器官移植基础实验 basic experiments of organ transplantation • 上一篇    下一篇

促胰素对大鼠胰岛细胞增殖及功能的影响★

徐艳花1，王洪敏2，张  振1，蔡德鸿1，于静雯1，吴礼凤1，陈道明2，陈  宏1   

  1. 1南方医科大学附属珠江医院内分泌科，广东省广州市  510282；2解放军第四五八医院全军肝病中心，广东省广州市  
    510600
  • 收稿日期:2011-10-28 修回日期:2011-12-16 出版日期:2012-01-29 发布日期:2012-01-29
  • 通讯作者: 陈宏，主任医师，南方医科大学珠江医院内分泌科，广东省广州市 510282 rubychq@163.com
  • 作者简介:徐艳花★，女，1985年生，江西省九江市人，汉族，南方医科大学在读硕士，主要从事胰岛再生与移植研究。 xyylvls@163.com

Effect of pancreatotrophin on the proliferation and function of islet cells in rats

Xu Yan-hua1, Wang Hong-min2, Zhang Zhen1, Cai De-hong1, Yu Jing-wen1, Wu Li-feng1, Chen Dao-ming1, Chen Hong1   

  1. 1Department of Endocrinology, Affiliated Zhujiang Hospital of Southern Medical University, Guangzhou  510282, Guangdong Province, China; 2Center of Liver Disease, the 458 Hospital of Chinese PLA, Guangzhou  510600, Guangdong Province, China
  • Received:2011-10-28 Revised:2011-12-16 Online:2012-01-29 Published:2012-01-29
  • Contact: Chen Hong, Chief physician, Department of Endocrinology, Affiliated Zhujiang Hospital of Southern Medical University, Guangzhou 510282, Guangdong Province, China rubychq@163.com
  • About author:Xu Yan-hua★, Studying for master’s degree, Department of Endocrinology, Affiliated Zhujiang Hospital of Southern Medical University, Guangzhou 510282, Guangdong Province, China xyylvls@163.com

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摘要：

背景：有研究报道，促进胰岛β细胞增殖分化和再生是2型糖尿病患者一种潜在的治疗方案。
目的：观察促胰素对体外培养大鼠胰岛细胞增殖及功能的影响。
方法：采用胶原酶消化和组织培养法分离纯化大鼠胰岛细胞，检测其纯度和活性，将胰岛细胞分为空白对照组和实验组，空白对照组加入普通培养基，实验组培养基中加入不同质量浓度(100，200，300，400 mg/L)促胰素，培养1，3，5 d后CCK-8法检测细胞增殖活性；培养5 d后行低糖和高糖刺激胰岛素释放实验。
结果与结论：随着促胰素质量浓度的增高，胰岛细胞增殖活性呈剂量依赖性增加(P < 0.05)，但无明显时间依赖性。除100，200 mg/L组外，300，400 mg/L两组胰岛素分泌量均显著高于空白对照组(P < 0.05)。表明300，400 mg/L促胰素不仅可促进胰岛细胞增殖，而且可显著增强胰岛细胞分泌胰岛素的功能。
关键词：促胰素；胰岛细胞；增殖；胰岛功能；胰岛分离；纯化
doi:10.3969/j.issn.1673-8225.2012.05.027

关键词: 促胰素, 胰岛细胞, 增殖, 胰岛功能, 胰岛分离, 纯化

Abstract:

BACKGROUND: Studies have reported to promote proliferation, differentiation and regeneration of β-cells is a potential treatment for patients with type 2 diabetes.
OBJECTIVE: To investigate the effects of pancreatotrophin on the proliferation and function of rat islet cells in vitro.
METHODS: The rat islet cells were isolated and purified by collagenase digestion and tissue-culture, followed with the detection of purity and activity. The islet cells were divided into blank control group and experimental group. Normal culture medium was added in the blank control group, pacreatotrophin in different concentrations were put in the experimental groups (100, 200, 300, 400 mg/L respectively). CCK-8 detection was performed to test the proliferous activity after 1, 3 and 5 days. After 5 days of culture, insulin release test was carried out in the condition of low-glucose and high-glucose.
RESULTS AND CONCLUSION: Along with the increase of the pancreatotrophin concentration, the proliferate activity of islet cells was in a dose-dependent manner (P < 0.05), but it demonstrated that there was no obvious time-dependent; In addition to 100 mg/L group and 200 mg/L group, the volume of insulin secretion in 300 mg/L group and 400 mg/L group were significantly higher than that in the blank control group (P < 0.05). It demonstrates that the concentrations of pancreatotrophin in 300 mg/L and 400 mg/L can not only promote cell proliferation, but also can significantly enhance the function of islet cells to secrete insulin

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