中国组织工程研究

中国组织工程研究 ›› 2011, Vol. 15 ›› Issue (45): 8391-8394.doi: 10.3969/j.issn.1673-8225.2011.45.007

• 脂肪干细胞 adipose-derived stem cells • 上一篇    下一篇

兔脂肪干细胞在含有转化因子和转铁蛋白培养基中向软骨细胞的分化

杨国庆1，王兆杰2，安荣泽2，赵俊延2，齐新文2   

  1. 1湄潭县医院外科，贵州省遵义市 564100
    2遵义医学院第五附属(珠海)医院骨科，广东省珠海市519100
  • 收稿日期:2011-07-29 修回日期:2011-09-05 出版日期:2011-11-05 发布日期:2011-11-05
  • 通讯作者: 王兆杰，博士，主任医师，教授，遵义医学院第五附属(珠海)医院骨科，广东省珠海市519100 zhaojiewang@163.com
  • 作者简介:杨国庆，男，1956年生，贵州省贵阳市人，汉族，1980年遵义医学院毕业，副主任医师，主要从事骨与关节损伤修复及重建的研究。 Yangguoqing6666@sina.com
  • 基金资助:

    贵州省科技厅资助项目(黔科通NY字[2006]62-2)；珠海市医学重点专科建设基金资助(珠卫[2008]80)。

Chondrogenic differentiation of rabbit adipose-derived stem cells in culture medium containing transforming factors and transferrin

Yang Guo-qing1, Wang Zhao-jie2, An Rong-ze2, Zhao Jun-yan2, Qi Xin-wen2   

  1. 1Department of Surgery, Meitan County Hospital, Zunyi  564100, Guizhou Province, China
    2Department of Orthopedics, Fifth Affiliated Hospital of Zunyi Medical College, Zhuhai  519100, Guangdong Province, China
  • Received:2011-07-29 Revised:2011-09-05 Online:2011-11-05 Published:2011-11-05
  • Contact: Wang Zhao-jie, Doctor, Chief physician, Professor, Department of Orthopedics, Fifth Affiliated Hospital of Zunyi Medical College, Zhuhai 519100, Guangdong Province, China zhaojiewang@163.com
  • About author:Yang Guo-qing, Associate chief physician, Department of Surgery, Meitan County Hospital, Zunyi 564100, Guizhou Province, China yangguoqing6666@sina.com
  • Supported by:

    Guizhou Science and Technology Bureau, No. [2006]62-2*; the Medical Key Foundation of Zhuhai City, No. [2008]80*

PDF

320

被引次数

5

摘要：

背景：成软骨的种子细胞的选择是软骨组织工程研究中的关键因素。
目的：观察脂肪干细胞在含有转化因子和转铁蛋白诱导条件下向软骨细胞分化的能力。
方法：取新西兰大白兔颈背部脂肪组织，机械分离及酶消化法获得脂肪干细胞，显微镜下观察细胞黏附及生长情况；加入含有转化因子β1和转铁蛋白的诱导培养基培养2周后以免疫组化方法检测Ⅱ型胶原的表达。
结果与结论：从兔脂肪组织中分离出的干细胞原代培养时24 h贴壁，96 h后达80%融合；于软骨诱导培养基内向软骨诱导7 d后形成软骨结节，14 d后Ⅱ型胶原免疫组化阳性。结果初步表明兔脂肪干细胞经诱导后可以向软骨细胞分化。

关键词: 脂肪干细胞, 软骨细胞, 软骨组织工程, 分化, 兔

Abstract:

BACKGROUND: The choice of seed cells is a key factor to the cartilage tissue engineering.
OBJECTIVE: To explore the chondrogenic potential of adipose-derived stem cells (ADSCs) in culture medium containing transforming factors and transferring.
METHODS: ADSCs were obtained from the subcutaneous adipose tissue of 6-month-old New Zealand white rabbits’ neck back by mechanical digestion and enzyme digestion, and then cultured and amplified in vitro. The adhesion and growth of cells were observed using inverted phase contrast microscope. Two weeks after the cells were cultured in chondrogenic medium, collagen type Ⅱ expression was detected by immunohistochemistry staining.
RESULTS AND CONCLUSION: The primary stem cells isolated from adipose tissue adhered on the plate in 24 hours, and reached 90% confluence in single layers after 96 hours. The cartilage nodules were observed at 7 days after ADSCs were cultured under chondrogenic medium, while collagen type Ⅱ was positive 14 days later. ADSCs of rabbits can express chondrogenic phenotype when they were cultured in chondrogenic medium, which proves the ADSCs can be one of the options for cartilage tissue engineering.

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