中国组织工程研究

中国组织工程研究 ›› 2011, Vol. 15 ›› Issue (45): 8395-8398.doi: 10.3969/j.issn.1673-8225.2011.45.008

• 肿瘤干细胞 cancer stem cells • 上一篇    下一篇

LMP1-CTAR3对鼻咽癌干细胞SP18生长的影响

全  胜1，2，庄英帜1，张志伟3，刘重元3，姜  浩1，赵  强4，朱建思3，董  琳3   

  1. 1南华大学附属第一医院肿瘤内科，湖南省衡阳市 421001
    2湖南环境生物职业技术学院，湖南省衡阳市  421001
    3南华大学医学院肿瘤研究所，湖南省衡阳市421001
    4南华大学附属第一医院病理科，湖南省衡阳市421001
  • 收稿日期:2011-07-26 修回日期:2011-09-07 出版日期:2011-11-05 发布日期:2011-11-05
  • 通讯作者: 庄英帜，主任医师，南华大学附属第一医院肿瘤内科，湖南省衡阳市 421001 988696@qq.com 并列通讯作者：张志伟，硕士，副教授，南华大学医学院肿瘤研究所，湖南省衡阳市421001 zhanghiweichina@yahoo.com.cn
  • 作者简介:全胜，男，1981年生，湖南省衡阳市人，汉族，2005年南华大学毕业，讲师，主要从事肿瘤内科临床与科研。 22029735@qq.com
  • 基金资助:

    湖南省高校创新平台开放基金(10K052)，湖南省重点学科建设基金(2006-180)，湖南省研究生科研创新项目(CX2010B380)，湖南省大学生研究性学习和创新性实验计划项目(CXSY-SJ-09007)。

The growth of nasopharyngeal cancer stem cell SP18 affected by carboxy terminal activating region-3 of the Epstein-Barr virus encoded latent membrane protein 1

Quan Sheng1, 2, Zhuang Ying-zhi1, Zhang Zhi-wei3, Liu Zhong-yuan3, Jiang Hao1, Zhao Qiang4, Zhu Jian-si3, Dong Lin3   

  1. 1Department of Oncology, the First Affiliated Hospital, University of South China, Hengyang  421001, Hunan Province, China
    2Hunan Environment- Biological Polytechnic College, Hengyang  421001, Hunan Province, China
    3Cancer Research Institute of Medical College, University of South China, Hengyang  421001, Hunan Province, China
    4Department of Pathology, the First Affiliated Hospital, University of South China, Hengyang  421001, Hunan Province, China
  • Received:2011-07-26 Revised:2011-09-07 Online:2011-11-05 Published:2011-11-05
  • Contact: Zhuang Ying-zhi, Chief physician, Department of Oncology, the First Affiliated Hospital, University of South China, Hengyang 421001, Hunan Province, China 988696@qq.com Correspondence to: Zhang Zhi-wei, Master, Associate professor, Cancer Research Institute of Medical College, University of South China, Hengyang 421001, Hunan Province, China zhanghiweichina@yahoo.com.cn
  • About author:Quan Sheng, Lecturer, Department of Oncology, the First Affiliated Hospital, University of South China, Hengyang 421001, Hunan Province, China; Hunan Environment- Biological Polytechnic College, Hengyang 421001, Hunan Province, China 22029735@qq.com
  • Supported by:

    Innovation Platform Open Foundation of Hunan Universities, No. 10K052*; the Key Discipline Construction Fund of Hunan Province, No. 2006-180*; Graduate Research and Innovation Project of Hunan Province, No. CX2010B380*; College Students Learning and Innovative Research Project of Hunan Province, No. CXSY-SJ-09007*

PDF

310

被引次数

4

摘要：

背景：课题组前期构建了CTAR3缺失突变型LMP1(LMP1△232-351)表达载体，初步了解该活性区在LMP1发挥作用中扮演十分重要的角色。
目的：观察LMP1-CTAR3对鼻咽癌干细胞SP18生长的影响。
方法：采用逆病毒感染方式，建立稳定表达LMP1及CTAR3缺失突变型LMP1(LMP1△232-351)的鼻咽癌干细胞SP18细胞系，通过细胞生长曲线、软琼脂集落形成实验和平皿克隆形成实验观察LMP1△232-351对鼻咽癌干细胞SP18生长的影响，然后选用流式细胞术检测细胞周期，计算细胞增殖指数。另外，利用Western-blot检测细胞中JAK3蛋白的磷酸化水平。
结果与结论：①与SP18-LMP1细胞比较，SP18-LMP1△232-351细胞生长速度减慢，增殖指数降低(P < 0.01)。②在SP18-  LMP1△232-351细胞中JAK3的磷酸化水平低于SP18- LMP1细胞。结果说明LMP1-CTAR3可能通过影响SP18细胞中JAK3的磷酸化，下调细胞的增殖指数，降低促细胞增殖的能力。

关键词: 鼻咽癌干细胞SP18, LMP1, CTAR3, 生长增殖, JAK3蛋白

Abstract:

BACKGROUND: Carboxy terminal activating region-3 of the Epstein-Barr virus encoded latent membrane protein 1 (LMP1-CTAR3) has been constructed in the previous studies. CTAR3 plays an important role in LMP1.
OBJECTIVE: To observe the effect of LMP1-CTAR3 on the growth of nasopharyngeal cancer stem cell SP18.
METHODS: We adopted the method of retroviruses infection and established the nasopharyngeal cancer stem cell SP18 of stable expression LMP1 and LMP1△232-351. Then, we made use of cell growth curve, plate clone formation and soft agar colony experiment to observe the growth of nasopharyngeal cancer stem cell SP18 affected by LMP1△232-351. Meanwhile, we detected the cycle of SP18-LMP1△232-351 cell by flow cytometry and calculated cellular proliferation index. In addition, Western-blot detection was used to determine phosphorylation JAK3 protein.
RESULTS AND CONCLUSION: Compared with SP18-LMP1 cells, the growth velocity and proliferation index of SP18-LMP1△232-351 cells obviously decreased (P < 0.01). The expression of phosphorylation JAK3 protein in SP18-LMP1△232-351 cells was lower than that in SP18-LMP1 cells. LMP1-CTAR3 probably affected the expression of phosphorylation JAK3 protein in SP18 to decrease cellular proliferation index and reduce the ability of cell proliferation promoted by LMP1.

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