中国组织工程研究

中国组织工程研究 ›› 2011, Vol. 15 ›› Issue (45): 8386-8390.doi: 10.3969/j.issn.1673-8225.2011.45.006

• 脂肪干细胞 adipose-derived stem cells • 上一篇    下一篇

骨形态发生蛋白2复合脂肪源性干细胞修复兔缺血性股骨头坏死

齐新文，王兆杰，陈军平，赵俊延，安荣泽   

  1. 遵义医学院第五附属(珠海)医院骨科，广东省珠海市  519100 
  • 收稿日期:2011-07-27 修回日期:2011-09-13 出版日期:2011-11-05 发布日期:2011-11-05
  • 通讯作者: 王兆杰，博士，主任医师，遵义医学院第五附属(珠海)医院骨科，广东省珠海市 519100 c3h3qxw@163.com
  • 作者简介:齐新文★，男，1973年生，汉族，2004年遵义医学院外科系骨科专业毕业，硕士，副主任医师，主要从事股骨头坏死和骨质疏松等骨病及创伤脊柱骨科方面的研究。
  • 基金资助:

    珠海市卫生局科研课题立项资助(2009069)； 遵义医学院2009年度青年科研启动基金课题资助[2009(05)]；珠海市医学重点建设专科基金资助项目(珠卫2008[80])。

Bone morphogenetic protein-2 combined with adipose derived stem cells for treatment of avascular necrosis of the femoral head in rabbits

Qi Xin-wen, Wang Zhao-jie, Chen Jun-ping, Zhao Jun-yan, An Rong-ze   

  1. Department of Orthopedics, the Fifth Affiliated Hospital of Zunyi Medical College, Zhuhai  51900, Guangdong Province, China
  • Received:2011-07-27 Revised:2011-09-13 Online:2011-11-05 Published:2011-11-05
  • Contact: Wang Zhao-jie , Doctor, Chief physician, Department of Orthopedics, the Fifth Affiliated Hospital of Zunyi Medical College, Zhuhai 51900, Guangdong Province, China c3h3qxw@163.com.cn
  • About author:Qi Xin-wen★, Master, Associate chief physician, Department of Orthopedics, the Fifth Affiliated Hospital of Zunyi Medical College, Zhuhai 51900, Guangdong Province, China
  • Supported by:

    Zhuhai Municipal Health Bureau of Scientific Research Project, No. 2009069*; 2009 Annual Youth Scientific Research Fund Project of Zunyi Medical College, No. 2009(05) *; Medical Development Project of Zhuhai City, No. 2008[80]*

PDF

341

被引次数

11

摘要：

背景：骨形态发生蛋白2和脂肪源性干细胞都有很强的成骨能力，对于缺血性股骨头坏死的修复有重要意义。
目的：观察骨形态发生蛋白2体内复合脂肪源性干细胞成骨治疗兔股骨头缺血坏死的疗效。
方法：48只新西兰大白兔采用液氮冷冻法造模，随机分为对照组，髓芯减压组，脂肪源性干细胞组，骨形态发生蛋白2复合脂肪源性干细胞组。
结果与结论：脂肪源性干细胞组与骨形态发生蛋白2复合脂肪源性干细胞组兔股骨头坏死区域的组织修复旺盛，新生骨小梁较多且逐步强化，X射线未见明显的囊性变和股骨头塌陷发生。在8周时骨形态发生蛋白2复合脂肪源性干细胞组骨小梁及骨髓组织生长较单纯脂肪源性干细胞组更旺盛。表明骨形态发生蛋白2因子复合脂肪源性干细胞植入股骨头坏死模型后成骨较多，修复坏死区域功能较强。

关键词: 股骨头坏死, 细胞移植, 脂肪源性干细胞, 骨形态发生蛋白, 兔

Abstract:

BACKGROUND: Adipose derived stem cells (ADSCs) and bone morphogenetic protein-2 (BMP-2) have a strong osteogenic capability that is important for the repair of avascular necrosis of the femoral head.
OBJECTIVE: To observe the combined effects of ADSCs and BMP-2 on avascular necrosis of the femoral head.
METHODS: The avascular femoral head necrosis models were established in 48 rabbits using liquid nitrogen. The rabbits were divided into control group, core decompression group, ADSCs group and BMP-2+ADSCs group randomly. 
RESULTS AND CONCLUSION: Tissue repair of femoral head necrosis in ADSCs group and BMP-2+ADSCs group was vigorous. There were more newly formed trabeculae, which were strengthened. X-ray showed no obvious cystic degeneration and collapse of the femoral head. The growth of trabeculae and bone marrow in BMP-2+ADSCs group was more vigorous than that in ADSCs group at 8 weeks. It is indicated that BMP-2 combined with ADSCs have a stronger repairable ability in necrosis of the femoral head necrosisl.

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