中国组织工程研究

中国组织工程研究 ›› 2021, Vol. 25 ›› Issue (2): 196-200.doi: 10.3969/j.issn.2095-4344.2977

• 肌肉肌腱韧带组织构建 tissue construction of the muscle, tendon and ligament • 上一篇    下一篇

骨骼肌钝挫伤兔模型制备与打击力度的关系

刘  杏，魏晓菡，邓  洁，李仲铭   

  1. 昆明医科大学基础医学院人体解剖学与组织胚胎学系，云南省昆明市   650031
  • 收稿日期:2019-12-09 修回日期:2019-12-12 接受日期:2020-01-20 出版日期:2021-01-18 发布日期:2020-11-21
  • 通讯作者: 李仲铭，硕士，副教授，硕士生导师，昆明医科大学基础医学院人体解剖学与组织胚胎学系，云南省昆明市 650031
  • 作者简介:刘杏，女，1992年生，河南省太康县人，汉族，昆明医科大学基础医学院在读硕士，主要从事骨骼肌损伤修复的影像学研究。
  • 基金资助:
    云南省科技厅-省应用基础研究(昆医联合专项)[2017FE468(-189)]

Preparing a blunt contusion model of rabbit skeletal muscle under different blow strengths

Liu Xing, Wei Xiaohan, Deng Jie, Li Zhongming    

  1. Department of Human Anatomy and Histology and Embryology, Basic Medical College of Kunming Medical University, Kunming 650031, Yunnan Province, China
  • Received:2019-12-09 Revised:2019-12-12 Accepted:2020-01-20 Online:2021-01-18 Published:2020-11-21
  • Contact: Li Zhongming, Master, Associate professor, Master’s supervisor, Department of Human Anatomy and Histology and Embryology, Basic Medical College of Kunming Medical University, Kunming 650031, Yunnan Province, China
  • About author:Liu Xing, Master candidate, Department of Human Anatomy and Histology and Embryology, Basic Medical College of Kunming Medical University, Kunming 650031, Yunnan Province, China
  • Supported by:
     Yunnan Provincial Department of Science and Technology-Provincial Applied Basic Research (Kunming Medical Special Project), No. 2017FE468 (-189)

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摘要：

文题释义：
骨骼肌钝挫伤：是最常见的肌肉损伤类型之一，发生在组织受到快速而强大的压力，如直接且非穿透性撞击肌腹的情况下通常会导致肌肉的钝挫伤，进而导致肌肉内血肿。这类损伤经常发生在如足球、篮球及手球等团队运动中，且损伤后症状多样，主要表现为肌肉酸痛、活动受限等。

背景：骨骼肌钝挫伤模型的研究多以大鼠为实验对象，作者认为以兔为实验对象可以更好地观察其宏观及影像学变化。
目的：以自制重物下砸仪为实验基础，根据不同打击高度，制备骨骼肌损伤兔模型，希望为后续兔腓肠肌钝挫伤模型复制提供参考数据。
方法：33只新西兰兔随机分为4组，正常组3只，75 cm高度打击组、50 cm高度打击组、25 cm高度打击组各10只，除正常组外均进行不同力度的打击，制备骨骼肌损伤兔模型。通过大体观察、苏木精-伊红染色、Masson 染色、超声影像等结果对比不同高度打击后的骨骼肌损伤程度。
结果与结论：①从50 cm高度进行打击，可对骨骼肌造成中度损伤，其自然恢复时间大于21 d，适合于骨骼肌钝挫伤模型的建立；②75 cm高度打击易造成骨折，动物死亡较多；③25 cm高度打击，损伤较轻，7 d即可恢复，不适合用于骨骼肌损伤模型的建立。
https://orcid.org/0000-0001-6570-2052 (刘杏) 

中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程

关键词: 骨骼肌,  钝挫伤,  重物下砸仪,  二维超声,  影像学,  Masson染色,  兔,  实验

Abstract: BACKGROUND: In current studies regarding skeletal muscle blunt contusion model, rats are often taken as the experimental object. The authors believe that rabbits are the better experimental object to observe the macroscopic and imaging changes after blunt contusion.  

OBJECTIVE: Based on the self-made heavy-duty smashing instrument as the experimental basis, to replicate the skeletal muscle injury model according to different strike heights, which is expected to provide a reference basis for the subsequent replication of the rabbit gastrocnemius blunt contusion model.

METHODS: Thirty-three New Zealand rabbits were randomly divided into four groups: normal group (n=3), 75 cm strike group (n=10), 50 cm strike group (n=10), 25 cm strike group (n=10). Except for the normal group, all strikes were performed with different strengths. The severity of skeletal muscle damage after different strikes was compared through gross observation, hematoxylin-eosin staining, Masson staining, and ultrasound imaging.  

RESULTS AND CONCLUSION: The 50 cm height blow can cause moderate damage to the skeletal muscle, and the natural recovery time is over 21 days, which is suitable for the establishment of skeletal muscle blunt contusion model. The 75 cm height blow is likely to cause fractures, resulting in more deaths. The 25 cm height blow can cause mild damage that can be recovered within 7 days, which is not suitable for the establishment of skeletal muscle injury model.

Key words: skeletal muscle, blunt contusion, heavy-duty instrument, two-dimensional ultrasound, imaging, Masson staining, rabbit, experiment

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