中国组织工程研究

中国组织工程研究 ›› 2011, Vol. 15 ›› Issue (38): 7100-7104.doi: 10.3969/j.issn.1673-8225.2011.38.016

• 材料力学及表面改性 material mechanics and surface modification • 上一篇    下一篇

表面改性聚合物左旋聚乳酸-多聚赖氨酸可促进骨髓基质细胞的初始黏附

毛学理1，凌均棨1，肖  殷2，彭  慧3   

  1. 1中山大学光华口腔医学院附属口腔医院，广东省广州市  510055
    2澳大利亚昆士兰科技大学健康与生物医学研究所，澳大利亚昆士兰州布里斯班 4059
    3澳大利亚昆士兰大学生物工程与纳米技术研究所，澳大利亚昆士兰州布里斯班  4059
  • 收稿日期:2011-06-30 修回日期:2011-07-26 出版日期:2011-09-17 发布日期:2011-09-17
  • 通讯作者: 凌均棨，教授，中山大学光华口腔医学院附属口腔医院，广东省广州市510055 lingjq@ mai.sysu.edu.cn
  • 作者简介:毛学理☆，女，1975年生，湖南省涟源市人，汉族，2009年中山大学光华口腔医学院毕业，博士，讲师，主要从事口腔再生医学研究。 maoxuel@mail.sysu.edu.cn
  • 基金资助:

    广东省自然科学基金资助项目(10451008901005929)，课题名称：RhoA 调空hMSCs在聚合物-Mg-Ca仿生表面成骨分化的机制研究。

Modified poly (L-lactic acid)-poly (L-lysine) polymer induces bone marrow stromal cells initial adhesion

Mao Xue-li1, Ling Jun-qi1, Xiao Yin2, Peng Hui3   

  1. 1Department of Operative Dentistry and Endodontics, Stomatology Hopital of Guanghua College of Stomatology, Sun Yat-sen University, Guangzhou  510055, Guangdong Province, China
    2Institute of Health and Biomedical Innovation, Queensland University of Technology, Brisbane, QLD 4059, Australia
    3Centre for Magnetic Resonance, Australian Institute for Bioengineering and Nanotechnology, University of Queensland, Brisbane, QLD 4072, Australia
  • Received:2011-06-30 Revised:2011-07-26 Online:2011-09-17 Published:2011-09-17
  • Contact: Ling Jun-qi, Professor, Department of Operative Dentistry and Endodontics, Stomatology Hopital of Guanghua College of Stomatology, Sun Yat-sen University, Guangzhou 510055, Guangdong Province, China lingjq@ mai.sysu.edu.cn
  • About author:Mao Xue-li☆, Doctor, Lecturer, Department of Operative Dentistry and Endodontics, Stomatology Hopital of Guanghua College of Stomatology, Sun Yat-sen University, Guangzhou 510055, Guangdong Province, China maoxuel@mail.sysu.edu.cn
  • Supported by:

    the Natural Science Foundation of Guangdong Province, No. 10451008901005929*

PDF

379

被引次数

4

摘要：

背景：通过增加表面活性基团对生物支架材料进行表面改性，可提高材料对细胞的亲和力，有效提高材料的细胞相容性。
目的：合成表面改性聚合膜左旋聚乳酸-多聚赖氨酸(PLLA-PLL)，并观察其对骨髓基质细胞黏附、增殖的影响。
方法：通过开环聚合反应合成不同组分高分子聚合膜PLLA139-PLL131，PLLA77-PLL72，PLLA45-PLL246，将人骨髓基质细胞接种至不同组分聚合膜PLLA-PLL表面、左旋聚乳酸及商品化的细胞培养板，寻找最佳PLLA-PLL组分。
结果与结论：与左旋聚乳酸比较，不同组分PLLA-PLL聚合膜细胞黏附量均升高，以PLLA77-PLL72聚合膜组增高显著(P < 0.05)，所以最佳组分为PLLA77-PLL72，连续培养结果显示PLLA77-PLL72聚合膜表面骨髓基质细胞骨架蛋白表达丰富，清晰有序，增殖实验也证实了PLLA77-PLL72聚合膜可促进骨髓基质细胞增殖。

关键词: 左旋聚乳酸, 多聚赖氨酸, 骨髓基质细胞, 黏附, 增殖

Abstract:

BACKGROUND: It is an effective strategy that integrating the activated ligands into the surface of biomaterial scaffolds to enhance and regulate the material affinity for cells and cell compatibility.
OBJECTIVE: To develop poly (L-lactic acid)-poly (L-lysine) (PLLA-PLL) to enhance cell initial adhesion on the polymer surface.
METHODS: Different composition polymer films (PLLA139-PLL131, PLLA77-PLL72, PLLA45-PLL246) were synthesized through ring-opening metathesis polymerization. BMSCs were seeded onto these different composition polymer surfaces ( PLLA and TCP served as the control), and detected the best composition of PLLA-PLL polymer film for cell adhesion and proliferation.
RESULTS AND CONCLUSION: PLLA-PLL films increased the number of initially attached BMSCs in both serum and serum free conditions compared with PLLA. Increased PLL content in the PLLA-PLL co-polymer led to increase cell attachment, significant difference changes were detected on copolymer PLLA77-PLL72 (P< 0.05). Continued culture confirmed that PLLA77-PLL72 enhanced cell proliferation. BMSCs cultured on the PLLA-PLL had a clear and well organized F-actin fibre expression.

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