中国组织工程研究

中国组织工程研究 ›› 2011, Vol. 15 ›› Issue (37): 6851-6854.doi: 10.3969/j.issn.1673-8225.2011.37.003

• 骨组织构建 bone tissue construction • 上一篇    下一篇

肿瘤坏死因子α干预小鼠成骨细胞cbfa1/runx2基因的表达

朱建华1，张  沁1，刘继光2   

  1. 1佳木斯大学附属口腔医院，黑龙江省佳木斯市  154007
    2佳木斯大学科技处，黑龙江省佳木斯市  154007
  • 收稿日期:2011-03-09 修回日期:2011-04-20 出版日期:2011-09-10 发布日期:2011-09-10
  • 通讯作者: 刘继光，博士，教授，佳木斯大学科技处，黑龙江省佳木斯市 154007 Liujg5550@163.com
  • 作者简介:朱建华，女，1959年生，黑龙江省佳木斯市人，汉族，教授，主要从事牙周黏膜病方面的研究。 zhu8622877@yahoo.com.cn
  • 基金资助:

    教育部科学技术研究项目(205041)，课题名称：PTHrP对牙胚发生作用机制的研究。

Tumor necrosis factor-alpha influences cbfa1/runx2 gene expression in mouse osteoblasts

Zhu Jian-hua1, Zhang Qin1, Liu Ji-guang2   

  1. 1Stomatological Hospital, Jiamusi University, Jiamusi  154007, Heilongjiang Province, China
    2Department of Science and Technology, Jiamusi University, Jiamusi  154007, Heilongjiang Province, China
  • Received:2011-03-09 Revised:2011-04-20 Online:2011-09-10 Published:2011-09-10
  • Contact: Liu Ji-guang, Doctor, Professor, Department of Science and Technology, Jiamusi University, Jiamusi 154007, Heilongjiang Province, China Liujg5550@163.com
  • About author:Zhu Jian-hua, Professor, Stomatological Hospital, Jiamusi University, Jiamusi 154007, Heilongjiang Province, China zhu8622877@yahoo.com.cn
  • Supported by:

    a grant from Science and Technology Program of Education Department of China, No. 205041*

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摘要：

背景：肿瘤坏死因子α可降低牙周膜纤维细胞碱性磷酸酶的活性，抑制牙周膜纤维细胞向成骨细胞的功能转化。
目的：观察肿瘤坏死因子α对小鼠成骨细胞生长及cbfa1/runx2基因表达的影响。
方法：取生长良好的小鼠成骨细胞系MC3T3/E1细胞，分别以20，40，60，80 μg/L的肿瘤坏死因子α进行干预，以正常培养的细胞作为对照。采用RT-PCR法检测MC3T3/E1细胞cbfa1/runx2 mRNA的表达；PNPP法测定碱性磷酸酶活性；MTT法检测细胞活力。
结果与结论：正常培养的MC3T3/E1细胞cbfa1/runx2 mRNA呈阳性表达，随着肿瘤坏死因子α浓度的增高，其表达水平逐渐下降。同时MC3T3/E1细胞活力和碱性磷酸酶活性也随肿瘤坏死因子α浓度的增高而下降。提示肿瘤坏死因子α可抑制MC3T3/E1细胞生长，而cbfa1/runx2可能参与了成骨细胞的分化过程。

关键词: 肿瘤坏死因子&alpha, MC3T3/E1细胞, cbfa1/runx2, 分化, 碱性磷酸酶

Abstract:

BACKGROUND: Tumor necrosis factor-alpha (TNF-α) can decrease alkaline phosphatase (ALP) activity in periodontal ligament fibroblasts and inhibit the functional transformation between periodontal ligament fibroblasts and osteoblasts.
OBJECTIVE: To investigate the effects of TNF-α on the growth of mouse osteoblasts and cbfa1/runx2 gene expression.
METHODS: Well growing mouse osteoblast line MC3T3/E1 were interfered with 20, 40, 60, 80 μg/L TNF-α. The normally cultured cells served as controls. cbfa1/runx2 mRNA expression in osteoblast line MC3T3/E1 was detected by RT-PCR. ALP activity was determined by PNPP method and cell viability was measured by MTT assay.
RESULTS AND CONCLUSION: cbfa1/runx2 mRNA expression was observed in the normally cultured osteoblast line MC3T3/E1. With increasing concentration of TNF-α concentration, cbfa1/runx2 mRNA expression, MC3T3/E1 cell viability and ALP activity were gradually decreased. Results suggested that TNF-α can inhibit osteoblast line MC3T3/E1 growth and cbfa1/runx2 may be involved in osteoblast differentiation.

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