中国组织工程研究

中国组织工程研究 ›› 2011, Vol. 15 ›› Issue (32): 6027-6031.doi: 10.3969/j.issn.1673-8225.2011.32.031

• 干细胞与中医药 stem cells and traditional Chinese medicine • 上一篇    下一篇

体外高浓度葡萄糖培养及黄芪干预人外周血内皮祖细胞的数量和增殖与分化

徐寒松1，雷闽湘2，孔德明1，向  慧3，谢晓云4   

  1. 1贵阳中医学院第二附属医院内分泌科，贵州省贵阳市  550003
    2中南大学湘雅医院内分泌科，湖南省长沙市  410008
    3贵州省人民医院心理科，贵州省贵阳市  550003
    4中南大学湘雅三医院内分泌科，湖南省长沙市410008
  • 收稿日期:2011-02-01 修回日期:2011-03-14 出版日期:2011-08-06 发布日期:2011-08-06
  • 作者简介:徐寒松☆，男，1973年生，贵州省安顺市人，汉族，2007年中南大学湘雅医院内科学内分泌专业毕业，博士，副教授，主要从事糖尿病慢性并发症研究。 xuhansong911@163.com
  • 基金资助:

    国家自然科学基金资助(30960491)；贵州省优秀科技教育人才省长基金资助。

Effects of high glucose and Astragalus Membranaceus on the number, proliferation, and differentiation of endothelial progenitor cells from human peripheral blood in vitro

Xu Han-song1, Lei Min-xiang2, Kong De-ming1, Xiang Hui3, Xie Xiao-yun4   

  1. 1Department of Endocrinology, the Second Affiliated Hospital of Guiyang College of TCM, Guiyang  550003, Guizhou Province, China
    2Department of Endocrinology, Xiangya Hospital of Central South University, Changsha  410008, Hunan Province, China
    3Department of Psychology, Guizhou Provincial People’s Hospital, Guiyang  550003, Guizhou Province, China
    4Department of Endocrinology, Third Xiangya Hospital of Central South University, Changsha  410008, Hunan Province, China
  • Received:2011-02-01 Revised:2011-03-14 Online:2011-08-06 Published:2011-08-06
  • About author:Xu Han-song☆, Doctor, Associate professor, Department of Endocrinology, the Second Affiliated Hospital of Guiyang College of TCM, Guiyang 550003, Guizhou Province, China xuhansong911@163.com
  • Supported by:

    the National Natural Science Foundation of China, No. 30960491*; Guizhou Grovernor Funding for Excellent Education Professionals*

PDF

364

被引次数

7

摘要：

背景：内皮祖细胞数量及功能受损是糖尿病血管并发症发生发展的重要环节，黄芪对多种原因引起内皮功能障碍具有保护作用，可以有效保护血管内皮功能。
目的：观察高浓度葡萄糖及黄芪干预对人外周血内皮祖细胞数量、增殖及分化影响。
方法：不同浓度葡萄糖孵育内皮祖细胞24 h以及30 mmol/L葡萄糖孵育内皮祖细胞不同时间;内皮祖细胞经20 g/L黄芪预处理24 h后，再加入30 mmol/L葡萄糖培养24 h。
结果与结论：高葡萄糖以浓度及时间依赖方式减少内皮祖细胞的数量，降低内皮祖细胞的增殖及向内皮细胞系分化能力(P  < 0.05或0.01)，给予黄芪预处理后，内皮祖细胞数量明显增加，增殖及向内皮细胞系分化能力明显增强(P < 0.05或0.01)。提示高葡萄糖以浓度及时间依赖方式减少外周血内皮祖细胞数量，降低内皮祖细胞的增殖及向内皮细胞系分化能力，黄芪可以改善高葡萄糖对内皮祖细胞的损伤作用。

关键词: 内皮祖细胞, 黄芪, 葡萄糖, 增殖, 分化

Abstract:

BACKGROUND: The quantity and impaired function of endothelial progenitor cells (EPCs) is the important link of diabetic vascular complications. Astragalus Membranaceus (AMI) can protect endothelium of dysfunction which caused by various reasons and can effectively protect the function of vascular endothelium.
OBJECTIVE: To investigate the effects of high glucose and AMI on the number, proliferation, and differentiation of EPCs from human peripheral blood in vitro.
METHODS: EPCs were incubated with various concentrations of glucose for 24 hours and with 30 mmol/L glucose for different periods. Simultaneously, EPCs were pre-incubated with 20 g/L AMI for 24 hours and then all the cells were incubated with 30 mmol/L glucose for 24 hours.
RESULTS AND CONCLUSION: High glucose decreased the number of EPCs and abilities of EPCs proliferation and differentiation in a dose- and time-dependent manner (P < 0.05 or 0.01). Pre-incubated with AMI could increase EPCs quantity and improve EPCs proliferative and differentiative capacity (P < 0.05 or 0.01). AMI can increase the number and improve the capacity of proliferation and differentiation of EPCs cultured with high glucose.

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