中国组织工程研究

中国组织工程研究 ›› 2011, Vol. 15 ›› Issue (31): 5831-5834.doi: 10.3969/j.issn.1673-8225.2011.31.031

• 移植与基因 transplantation and gene • 上一篇    下一篇

精子发生相关基因及凋亡基因Dazl、Pgk2、Prm2、bax在卵巢异性移植小鼠睾丸、附睾组织中的表达

彭南妮1,2，薛立群1, 袁安文1，杨  青1，凌理军1，吴  珊1，邹红艳2，吴方贵2   

  1. 1湖南农业大学动物医学院与动物科技学院，湖南省长沙市 410128
    2深圳市第五人民医院生殖医学中心，广东省深圳市 518000
  • 收稿日期:2010-03-04 修回日期:2011-05-04 出版日期:2011-07-30 发布日期:2011-07-30
  • 通讯作者: 袁安文，博士，副教授，湖南农业大学动物医学系产科学教研室，湖南省长沙市 410128 yuananwen@ ahoo.com.cn
  • 作者简介:彭南妮☆，女，1964年生，湖南省新化市人，2009年湖南农业大学毕业，博士，副主任医师， nnpeng@126.com
  • 基金资助:

    湖南省重点科技项目(01NKY1002-01)，课题名称：现代农业生物技术研究与应用。湖南省科技项目(2007-TP4024)，课题名称：实验动物小鼠腔前卵泡冷冻保存与发育的研究。深圳罗湖区科技局资助项目(200806)，课题名称：卵巢组织的冻融移植和复苏卵母细胞体外培养。

Expression of spermatogenesis-related genes Dazl, Pgk2, Prm2 and apoptosis-related gene bax in testicle and epididymis tissue from ovarian graft heterosexual recipients

Peng Nan-ni1,2, Xue Li-qun1, Yuan An-wen1, Yan Qing1, Ling Li-jun1, Wu Shan1, Zou Hong-yan2, Wu Fang-gui2   

  1. 1Colleges of Veterinary Medicine and Animal Science & Technology, Hunan Agricultural University, Changsha 410128, Hunan Province, China; 2Reproductive Medical Center of Shenzhen Fifth People’s Hospital, Shenzhen 518000, Guangdong Province, China
  • Received:2010-03-04 Revised:2011-05-04 Online:2011-07-30 Published:2011-07-30
  • Contact: Yuan An-wen, Doctor, Associate professor, Colleges of Veterinary Medicine and Animal Science & Technology, Hunan Agricultural University, Changsha 410128, Hunan Province, China yuananwen@yahoo. com.cn
  • About author:Peng Nan-ni☆, Doctor, Associate chief physician, Colleges of Veterinary Medicine and Animal Science & Technology, Hunan Agricultural University, Changsha 410128, Hunan Province, China; Reproductive Medical Center of Shenzhen Fifth People’s Hospital, Shenzhen 518000, Guangdong Province, China nnpeng@126.com
  • Supported by:

    Key Science and Technology Program of Hunan Province, No.01NKY1002-01*; Science and Technology Program of Human Province, No. 2007-TP4024*;  a grant from Science and Technology Bureau of Luohu District of Shenzhen City, No.200806*

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摘要：

背景：Dazl、Pgk2、Prm2和细胞凋亡相关基因bax均参与了精子发生的调控，这些基因的表达异常和缺失常常影响精子的发生。
目的：探讨卵巢移植对异性受体精子发生相关基因表达的影响。
方法：将1 d龄小鼠卵巢移植入成年雄性受体鼠肾囊下，分激素处理或不做激素处理两个实验组；于卵巢移植后第21天观察卵巢移植体，选择卵泡发育良好的受体鼠分别采集雄鼠睾丸、附睾提取总RNA，采用半定量RT-PCR分析目的基因表达情况。对照组为未进行卵巢移植的同龄正常雄鼠。
结果与结论：各组间目的基因的表达除Prm2的相对表达量在激素处理移植组显著高于对照组外(P < 0.05)，其余各组间各基因的表达差异无显著性意义(P > 0.05)。初步证实在卵巢异性移植构建雌、雄性腺同体的生理环境中，卵巢移植体对受体雄鼠几种精子发生相关基因及凋亡基因的表达无明显影响。提示卵巢移植对雄性受体小鼠的精子发生在分子水平上无明显的损害作用。

关键词: 小鼠, 卵巢移植, 睾丸, 基因, 异性受体

Abstract:

BACKGROUND: Dazl, Pgk2, Prm2 and apoptosis-related gene bax are involved in the regulation of spermatogenesis. The abnormal expression and deletions of these genes often affect spermatogenesis, and even lead to infertility.
OBJECTIVE: To investigate the effects of ovarian transplantation on the expression levels of spermatogenesis-related genes in heterosexual recipients.
METHODS: Ovaries from 1-day-old mice were transplanted under the kidney capsule of male recipient mice, and recipients were treated with or without hormone. Ovarian grafts were observed 21 days after transplantation. Tissues including testis and epididymis were collected from mice with good developmental follicles. Total RNAs were extracted and expression levels of genes were determined using semi-quantitative RT-PCR. Normal male mice with the same age were used as controls.
RESULTS AND CONCLUSION: Gene Prm2 expression in the transplanted group treated with gonadotrophine was significantly higher than that in the control group (P < 0.05), and there was no significant difference in expression level of other genes between all groups (P  > 0.05). These data confirmed that ovarian grafts have no effects on the expression levels of spermatogenesis-and apoptosis-related genes in the mouse model with male and female gonads. This may suggest that ovarian transplantation has no damage to the spermatogenesis of male recipient mice at the molecular level.

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