中国组织工程研究

中国组织工程研究 ›› 2010, Vol. 14 ›› Issue (46): 8581-.doi: 10.3969/j.issn.1673-8225.2010.46.008

• 织构建与生物活性因子 • 上一篇    下一篇

大鼠磨牙加力移动龈沟液中核因子κB受体活化子配体/骨保护素的表达变化

张昀1，杨磊2，马钢1，张林棋1，高巍然2，张红霞1   

  1. 1白求恩军医学院附属解放军第260医院口腔科，河北省石家庄市  050041；2解放军264医院，山西省太原市  030001
  • 出版日期:2010-11-12 发布日期:2010-11-12
  • 通讯作者: 杨磊，硕士，主治医师，解放军264医院口腔科，山西省太原市 030001 taiyuandentist@sina.com
  • 作者简介:张昀★，女，1970年生，河北省石家庄市人，汉族，2006年解放军第四军医大学毕业，硕士，主治医师。主要从事正畸牙齿移动的临床及生物学基础研究。Bamboo9928@sina.com

Expression variation of the receptor activator for nuclear factor kappa B ligand and osteoprotegerin in the gingival crevicular fluid of rats with orthodontic appliances

Zhang Yun1, Yang Lei2, Ma Gang1, Zhang Lin-qi1, Gao Wei-ran2, Zhang Hong-xia1   

  1. 1Department of Stomatology, the 260 Hospital of Chinese PLA, Bethune Military Medical College, Shijiazhuang  050041, Hebei Province, China; 2the 264 Hospital of Chinese PLA, Taiyuan  030001, Shanxi Province, China
  • Online:2010-11-12 Published:2010-11-12
  • Contact: Yang Lei, Master, Attending physician, the 264 Hospital of Chinese PLA, Taiyuan 030001, Shanxi Province, China taiyuandentist@sina.com
  • About author:Zhang Yun★, Master, Attending physician, Department of Stomatology, the 260 Hospital of Chinese PLA, Bethune Military Medical College, Shijiazhuang 050041, Hebei Province, China Bamboo9928@sina.com

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摘要：

背景：核因子κB受体活化子配体和骨保护素系统参与破牙骨质细胞的形成并调节牙根吸收过程，而不同力值大小及加力时间下龈沟液中核因子κB受体活化子配体和骨保护素的表达变化是否可以作为判断牙根吸收的量化指标鲜有报道。
目的：探讨核因子κB受体活化子配体和骨保护素表达变化与力值大小及加力时间的相关性。
方法：雄性SD大鼠随机分成0.49 N，0.98 N和1.47 N力值组，每组按照加力时间分为加力0，3，7，10，14，21及28 d亚组。各组大鼠建立大鼠正畸牙模型，采用吸潮纸尖法提取实验牙的龈沟液，应用ELISA法测定不同力值和加力时间龈沟液核因子κB受体活化子配体和骨保护素的浓度及比值变化。
结果与结论：加力不同时间点不同力值加载组组间大鼠龈沟液中核因子κB受体活化子配体/骨保护素的浓度比值比较差异均具有显著性意义(P < 0.01)，力值越大，该比值越大。0.49 N加力组在加力的前21 d，随着加力时间的延长，大鼠龈沟液中核因子κB受体活化子配体/骨保护素的值逐渐增高(P < 0.01)；第28天的核因子κB受体活化子配体/骨保护素的比值趋于稳定。0.98 N和1.97 N加力组，随着加力时间的延长，大鼠龈沟液中核因子κB受体活化子配体/骨保护素的值均持续逐渐增高(P < 0.01)。结果表明，大鼠龈沟液中核因子κB受体活化子配体和骨保护素的比值与力值大小及作用时间具有相关性，可用于正畸牙根吸收的临床检测指标。

关键词: 龈沟液, 正畸牙, 核因子&kappa, B受体活化子配体, 骨保护素, 牙根吸收

Abstract:

BACKGROUND: Receptor activator of nuclear factor κ B ligand ligand (RANKL) and osteoprotegerin (OPG) play an important role in the formation of odontclasts and the root resorption. Whether the expression and changes of RANKL and OPG in gingival crevicular fluid (GCF) may be an index of root resorption in orthodontic tooth movement remains unclear.
OBJECTIVE: To investigate the changes and significance of the receptor activator for RANKL and OPG in GCF of the rats with orthodontic appliances.
METHODS: An animal model of orthodontic tooth movement was established in male SD rat. The rats were randomly divided into 0.49 N, 0.98 N, and 1.47 N groups, which assigned into 0, 3, 7, 10, 14, 21 and 28 days subgroups. Following careful sampling of GCF with absorbed paper points in the rats, the levels of RANKL and OPG were determined by ELISA. The effect of loading duration on RANKL and OPG ratio was observed.
RESULTS AND CONCLUSION: There were obviously differences in RANKL/OPG in different groups (P < 0.01). The RANKL/OPG was increased as the force increased. In the 0.49 N-force application group, the level of RANKL/OPG increased significantly during the first 21 days (P < 0.01), but the changes were not significant at 28 days. In the 0.98 N and 1.47 N-force application groups, the level of RANKL/OPG increased significantly between different orthodontic force application days (P < 0.01). RANKL and OPG in GCF play an important role in the root resorption in the rats with orthodontic appliances, which can be served as an clinical index for testing root resorption.

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