中国组织工程研究

中国组织工程研究 ›› 2010, Vol. 14 ›› Issue (46): 8576-.doi: 10.3969/j.issn.1673-8225.2010.46.007

• 织构建与生物活性因子 • 上一篇    下一篇

2型糖尿病伴牙周病大鼠牙槽骨中转化生长因子β1 mRNA的表达

曲攀，高秀秋   

  1. 辽宁医学院附属第二医院，辽宁省锦州市  121000
  • 出版日期:2010-11-12 发布日期:2010-11-12
  • 通讯作者: 高秀秋，硕士研究生导师，教授，辽宁医学院附属第二医院，辽宁省锦州市 121000 jzgaoxiuqiu1988@yahoo.com.cn
  • 基金资助:

    辽宁省教育厅基金（2008418），课题名称：2型糖尿病伴发牙周病大鼠IL-1基因多态性的研究。

Expression of transforming growth factor beta 1 mRNA in alveolar bone of rats with type 2 diabetes mellitus combined with periodontal disease

Qu Pan, Gao Xiu-qiu   

  1. Second Affiliated Hospital of Liaoning Medical University, Jinzhou  121000, Liaoning Province, China
  • Online:2010-11-12 Published:2010-11-12
  • Contact: Gao Xiu-qiu, Master’s supervisor, Professor, Second Affiliated Hospital of Liaoning Medical University, Jinzhou 121000, Liaoning Province, China jzgaoxiuqiu1988@yahoo.com.cn
  • Supported by:

    the Foundation of Education Department of Liaoning Province, No. 2008418*

PDF

461

摘要：

背景：研究表明糖尿病可以增加牙周病的发病风险，糖尿病可以引起骨代谢的紊乱并导致骨质疏松症，但其具体机制尚未阐明。
目的：通过对大鼠牙槽骨转化生长因子β1 mRNA表达及根尖区骨密度变化的测定，探究2型糖尿病促进牙周病变的机制。
方法：采用高糖高脂饮食喂养、小剂量链脲佐菌素、一次性腹腔注射及正畸结扎丝结扎的方法建立2型糖尿病伴牙周病SD大鼠模型。应用RT-PCR技术检测实验大鼠牙槽骨中转化生长因子β1 mRNA表达，并对大鼠牙槽骨进行骨密度测量及病理切片光镜观察。  
结果与结论：与正常对照组、2型糖尿病组、牙周病组比较，2型糖尿病伴牙周病组大鼠牙槽骨中转化生长因子β1 mRNA表达最高，骨密度值最低(P < 0.01)。2型糖尿病伴牙周病组大鼠牙槽骨有骨质疏松倾向。结果提示2型糖尿病可能通过转化生长因子β1的过表达来促进牙槽骨骨质疏松，进而说明2型糖尿病可能通过转化生长因子β1的过表达来促进牙周病变。

关键词: 糖尿病, 牙周病, 牙槽骨, 转化生长因子&beta, 1, 骨密度

Abstract:

BACKGROUND: Research showed that diabetes can increase the risk of periodontal disease, diabetes can cause the disorder of bone metabolism and lead to osteoporosis, but the specific mechanisms remain unclear.
OBJECTIVE: To explore the mechanism of type Ⅱ diabetes mellitus on promoting periodontal disease by measuring rat alveolar expression of transforming growth factor β1 mRNA (TGF-β1 mRNA) and apical bone mineral density.
METHODS: SD rats were prepared for type Ⅱ diabetes mellitus models by using high glucose and high fat diet, low-dose streptozotocin intraperitoneal injection and orthodontic ligation wire ligation. Expression of TGF-1 mRNA in alveolar bone was detected by RT-PCR, and the alveolar bone was performed bone mineral density measurement and pathological observation.
RESULTS AND CONCLUSION: Compared with other groups, the expression of TGF-1 mRNA was greatest, and the bone mineral density was smallest in the type 2 diabetes mellitus combined with periodontal disease group (P < 0.01). Type Ⅱ diabetic rats with periodontal disease tended to alveolar bone osteoporosis. The results suggest that type Ⅱ diabetes mellitus possibly promote alveolar bone osteoporosis through over-expression of TGF-β1, and type Ⅱ diabetes mellitus can also pass the transforming growth factor-β1 over-expression to promote periodontal disease.

中图分类号: