中国组织工程研究

中国组织工程研究 ›› 2010, Vol. 14 ›› Issue (10): 1843-1846.doi: 10.3969/j.issn.1673-8225.2010.10.028

• 干细胞转基因表达 transgenic expression in stem cells • 上一篇    下一篇

腺相关病毒介导绿色荧光蛋白基因体外转染人羊膜上皮细胞

罗宏武，黄湘俊，刘浔阳，黄飞舟   

  1. 中南大学湘雅三医院肝胆外科，湖南省长沙市 413000
  • 出版日期:2010-03-05 发布日期:2010-03-05
  • 通讯作者: 黄湘俊，硕士，医师，中南大学湘雅三医院肝胆外科，湖南省长沙市 413000 hxiangj168@ 126.com
  • 作者简介:罗宏武，男，1970年生，湖南省宁乡县人，汉族，2008年中南大学湘雅医学院毕业，博士，副主任医师，硕士生导师，主要从事肝胆外科方面的研究。 luohongwuzny@sina.com

Adeno-associated virus vector-medicated green fluorescent protein transfected human amniotic epithelial cells in vitro  

Luo Hong-wu, Huang Xiang-jun, Liu Xun-yang, Huang Fei-zhou   

  1. Department of Hepatobiliary Surgery, Third Xiangya Hospital, Central South University, Changsha   413000, Hunan Province, China
  • Online:2010-03-05 Published:2010-03-05
  • Contact: Huang Xiang-jun, Master, Physician, Department of Hepatobiliary Surgery, Third Xiangya Hospital, Central South University, Changsha 413000, Hunan Province, China hxiangj168@126. com
  • About author:Luo Hong-wu, Doctor, Associate chief physician, Master’s supervisor, Department of Hepatobiliary Surgery, Third Xiangya Hospital, Central South University, Changsha 413000, Hunan Province, China luohongwuzny@sina.com

PDF

515

被引次数

6

摘要：

背景：人羊膜上皮细胞易于获得，采集简单，是细胞移植和组织修复的理想种子细胞，目前国内外尚无关于标记、示踪体外培养的人羊膜上皮细胞的报道。
目的：探讨腺相关病毒载体介导绿色荧光蛋白基因对体外培养的人羊膜上皮细胞的转染效果。
方法：取人羊膜标本，以胰蛋白酶消化法分离培养人羊膜上皮细胞，采用含绿色荧光蛋白的腺相关病毒进行转染，检测其转染效率。
结果与结论：人羊膜上皮细胞可成功地在体外进行原代和传代培养，经含绿色荧光蛋白的腺相关病毒颗粒转染后，可稳定高效表达绿色荧光蛋白，转染效率达58%。

关键词: 腺相关病毒, 绿色荧光蛋白, 基因, 转染, 人羊膜上皮细胞

Abstract:

BACKGROUND: Human amniotic epithelial cells (AECs) are easy to obtain and can function as ideal seed cells for cell transplantation and tissue repair. Currently, marking and tracing of human AECs remains rarely reported.
OBJECTIVE: To explore the efficiency of adeno-associated virus (AAV) vector-medicated green fluorescent protein (GFP) on in vitro cultured human AECs transfection.
METHODS: Human amnion samples were harvested and trypsinized to isolate human AECs. The AECs were transfected with AAV-GFP, and the transfection efficiency was detected.
RESULTS AND CONCLUSION: Human AECs were successfully primary cultured and passaged in vitro. AAV-GFP-transfected AECs stably and highly expressed GFP, with a transfection efficiency of 58%.

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