软骨组织工程中种子细胞选择的研究热点

doi:10.12307/2021.151

中国组织工程研究 ›› 2021, Vol. 25 ›› Issue (31): 5059-5064.doi: 10.12307/2021.151

• 干细胞综述 stem cell review • 上一篇下一篇

软骨组织工程中种子细胞选择的研究热点

刘小刚，李甜，张舵

吉林大学第一医院整形美容外科，吉林省长春市 130021

收稿日期:2020-07-01 修回日期:2020-07-06 接受日期:2020-08-10 出版日期:2021-11-08 发布日期:2021-04-25
通讯作者: 张舵，主任医师，教授，博士生导师，吉林大学第一医院整形美容外科，吉林省长春市 130021
作者简介:刘小刚，男，1994年生，湖北省黄冈市人，汉族，吉林大学在读硕士，主要从事器官修复再造与软骨组织工程研究。
基金资助:
中国医学科学院医学与健康科技创新工程项目(2017-I2M-1-007)，项目参与人：张舵

Research hotspot of seed cell selection in cartilage tissue engineering

Liu Xiaogang, Li Tian, Zhang Duo

Department of Plastic Surgery, the First Hospital of Jilin University, Changchun 130021, Jilin Province, China

Received:2020-07-01 Revised:2020-07-06 Accepted:2020-08-10 Online:2021-11-08 Published:2021-04-25
Contact: Zhang Duo, Chief physician, Professor, Doctoral supervisor, Department of Plastic Surgery, the First Hospital of Jilin University, Changchun 130021, Jilin Province, China
About author:Liu Xiaogang, Master candidate, Department of Plastic Surgery, the First Hospital of Jilin University, Changchun 130021, Jilin Province, China
Supported by:
Chinese Academy of Medical Sciences Medical and Health Technology Innovation Project, No. 2017-I2M-1-007 (to ZD)

摘要/Abstract

摘要：

文题释义：

软骨组织工程：是一门结合生物医学和材料工程学等学科的交叉学科，将软骨细胞或干细胞等种子细胞经过体外培养，种植于生物相容性良好、可降解的支架材料中，最终将形成的组织工程软骨植入软骨缺损处以修复软骨的技术。
共培养技术：主要将2种及2种以上的细胞一起培养在体外的相同环境中，以模拟细胞之间相互联系、共同生长的体内环境，自从20世纪80年代以来，在细胞相关研究中被广泛应用。
背景：耳、鼻、喉、气管与关节等软骨缺损修复始终是临床治疗所面临的难点之一，软骨组织工程的出现，成为了软骨修复的热门发展方向，其中种子细胞是构建组织工程软骨的关键。
目的：文章拟对不同类型的种子细胞特点及其在软骨组织工程中的应用、存在的问题等研究进展做一综述，为软骨组织工程从实验向临床进行转化带来新的思路。
方法：第一作者通过检索中国知网、万方、PubMed、Scopus和 Web of Science 数据库的相关文献，以“种子细胞，组织工程，软骨细胞，干细胞，共培养”为中文检索词，以“seed cells，tissue engineering，chondrocytes，stem cells，co-culture”为英文检索词进行检索。最终选取符合标准的70篇文献进行综述。
结果与结论：①目前软骨组织工程的实验研究取得了大量的成果，但关于种子细胞的选择问题仍是一大难题，软骨细胞、各种类型的间充质干细胞、胚胎干细胞、诱导多能干细胞等，均具有不同程度的优势，同时也面临着不同的挑战；②各种种子细胞的发现、发展与应用均推动了软骨组织工程的进步，其中软骨细胞与干细胞共培养是目前比较热门且具有研究前景的种子细胞选择。
https://orcid.org/0000-0003-1174-4008(刘小刚)

中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程

关键词: 种子细胞, 软骨组织工程, 软骨细胞, 间充质干细胞, 胚胎干细胞, 共培养

Abstract: BACKGROUND: The repair of cartilage defects such as ears, noses, throats, trachea and joints has always been one of the difficulties faced by clinical treatment. The emergence of cartilage tissue engineering has become a popular development direction for cartilage repair, in which seed cells are used to construct tissue engineered cartilage key.
OBJECTIVE: This article intends to review the characteristics of various types of seed cells, their application in cartilage tissue engineering, and existing problems, and to bring new ideas to clinical transformation.
METHODS: The first author searched the relevant articles of CNKI, Wanfang, PubMed, Scopus and Web of Science databases, using “seed cells; tissue engineering; chondrocytes; stem cells; co-cultivation” as the Chinese and English search terms. Finally, 70 articles that met the criteria were selected for review.

RESULTS AND CONCLUSION: (1) Current experimental research on cartilage tissue engineering has achieved a lot of results, but the problem of selecting seed cells is still a big problem. Chondrocytes, various types of mesenchymal stem cells, embryonic stem cells, and induced pluripotent stem cells have different degrees of advantages, but also face different challenges. (2) The discovery, development and application of various seed cells have promoted the progress of cartilage tissue engineering. Among them, the method of co-culture of chondrocytes and stem cells is currently a popular and promising seed choice for research.

Key words: seed cells, cartilage tissue engineering, chondrocytes, mesenchymal stem cells, embryonic stem cells, co-culture

中图分类号:

刘小刚, 李甜, 张舵. 软骨组织工程中种子细胞选择的研究热点[J]. 中国组织工程研究, 2021, 25(31): 5059-5064.

Liu Xiaogang, Li Tian, Zhang Duo. Research hotspot of seed cell selection in cartilage tissue engineering[J]. Chinese Journal of Tissue Engineering Research, 2021, 25(31): 5059-5064.

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软骨组织工程中种子细胞选择的研究热点

Research hotspot of seed cell selection in cartilage tissue engineering

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