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    10 September 2015, Volume 19 Issue 37 Previous Issue    Next Issue
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    Transcription factor Runx2 induces matrix extracellular phosphoglycoprotein promoter expression in preosteoblasts 
    Sun Yu-jiao, Gong Chun-mei, Hao Jian-zhong, Sun Yan, Liu Xiao-ying
    2015, 19 (37):  5905-5910.  doi: 10.3969/j.issn.2095-4344.2015.37.001
    Abstract ( 205 )   PDF (930KB) ( 381 )   Save

    BACKGROUND: Matrix extracellular phosphoglycoprotein phosphorylated extracellular matrix glycoprotein (MEPE) gene plays an important role in bone mineralization and absorption as well as the balance of osteoblasts and osteoclasts. Studies on the function and regulatory mechanism of MEPE can provide new ideas for the treatment of osteoporosis.

    OBJECTIVE: To analyze the regulatory effects of transcription factor Runx2 on MEPE promoter in mouse preosteoblasts, thereby preliminarily studying the Runx2 effects in the process of bone formation and development. 

    METHODS: First of all, the Runx2 eukaryotic expression vector was built according to the gene sequence of Runx2 in Genebank; then the dual luciferase reporter assay was employed to analyze the effects of Runx2 on transcription activity of MEPE promoters with different lengths in order to determine the promoter region in which Runx2 has significant effect. Afterwards, the effects of Runx2 on transcipition activity of MEPE gene promoter which induced by three MAPK signaling pathway inhibitors were investigated. Finally, real-time PCR was used to analyze the expression activity of MEPE gene promoter regulated by Runx2.

    RESULTS AND CONCLUSION: We successfully constructed the Runx2 eukaryotic expression vector. Dual luciferase reporter assay showed that Runx2 could increase the transcription activity of MEPE gene promoter in preosteoblasts, and the fragment area in which Runx2 exhibited the more significant up-regulatory effectiveness was (-300 to +66)366 bp. Runx2 could increase the transcription activity of MEPE gene promoter by activating the MAPK single pathway. The real-time PCR verified that Runx2 increased the expression activity of MEPE gene promoter. These findings indicate that Runx2 can regulate the express of MEPE gene promoter by the MAPK single pathway, in order to build the basis for exploring the process of bone formation and development.

     中国组织工程研究杂志出版内容重点:组织构建;骨细胞;软骨细胞;细胞培养;成纤维细胞;血管内皮细胞;骨质疏松组织工程

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    Dynamical effect of fractures combined with brain injury on the bone healing and bone metabolism
    Zhou Qing, Liu Jin-lian, Liu Chao-qun, Zhou Yao-dong, Chen Hao
    2015, 19 (37):  5911-5915.  doi: 10.3969/j.issn.2095-4344.2015.37.002
    Abstract ( 226 )   PDF (833KB) ( 312 )   Save

    BACKGROUND: Peri-fracture nerve injury can inhibit osteoclast activity and promote early fracture healing.

    OBJECTIVE: To investigate dynamically the effects of traumatic brain injury on the bone mineral density, microstructure, biomechanics property and bone metabolism in rat models of fractures. 

    METHODS: Sixty-three male rats were randomly divided into three groups: sham group, simple fracture group and fracture combined with brain injury group. After 3, 6, and 3 months, the animals were sacrificed in batches under anesthesia, and then, the bones and serum specimens were used to detect the bone mineral density, microstructure, biomechanics property, serum cross-linked N-telopeptide of collagen type I and osteocalcin levels. 

    RESULTS AND CONCLUSION: Compared with the simple fracture group, the fracture combined brain injury group had significantly increased bone mineral density of the proximal tibia, bone volume fraction of the cancellous bone, trabecular thickness, cross-sectional area of tibial cortical bone and total area of the bone marrow, ultimate load and stress of the tibia, serum cross-linked N-telopeptide of collagen type I and osteocalcin levels at 3 and 6 weeks after modeling (P < 0.05), but no differences in the above-mentioned indexes were found among the three groups at 3 months after modeling. These findings indicate that traumatic brain injury can increase the bone mineral density at the fracture site, improve bone microstructure and enhance biomechanical properties, thereby promoting bone healing and bone metabolism at the fracture site.

    中国组织工程研究杂志出版内容重点:组织构建;骨细胞;软骨细胞;细胞培养;成纤维细胞;血管内皮细胞;骨质疏松组织工程

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    Osteogenic differentiation of nucleus puplousus cells co-cultured with autologous periosteal cells
    Yang Yu-ming, Yuan Feng, Lu Hai-tao, Zhang Jun-wei, Sheng Xiao-lei, Li Zhi-duo
    2015, 19 (37):  5916-5922.  doi: 10.3969/j.issn.2095-4344.2015.37.003
    Abstract ( 237 )   PDF (5687KB) ( 705 )   Save

    BACKGROUND: Periosteal cells have been used in bone repair, but whether nucleus puplousus cells co-cultured with autologous periosteal cells can differentiate into osteoblasts in spinal fusion is rarely reported.

    OBJECTIVE: To isolate nucleus puplousus cells and periosteal cells so as to observe the osteogenic ability of nucleus puplousus cells co-cultured with periosteal cells or not.

    METHODS: Type II collagenase digestion method was used to isolate and purify nucleus pulposus cells, which were confirmed by toluidine blue and immunohistochemical staining. Periosteal cells were isolated histologically 
    and cultured in complete medium, and cell surface antigens CD90, CD105 were identified by immunofluorescence staining. According to the experimental needs, the cells were assigned into two groups. Nucleus pulposus cells and periosteal cells were co-cultured by osteogenic induction medium in the experimental group. Nucleus pulposus cells in the control group were cultured alone in osteogenic induction medium. Cell morphology was observed by inverted microscopy, and cell proliferation was detected by cell counting kit-8. The osteogenic differentiation indexes of cells in each group were measured using alkaline phosphatase staining, alizarin red staining, and type I collagen immunohistoehemical staining. The expression of osteopontin was tested by western blot assay.

    RESULTS AND CONCLUSION: CD105 and CD90 expressions of the periosteal cells were positive. Nucleus puplousus cells were positive for toluidine blue and collagen type II immunohistochemical staining. The proliferative ability of nucleus puplousus cells was significantly higher in the experimental group than the control group at days 1, 3, 5, 7, 9. After 2 weeks of induction, the cells were positive for alkaline phosphatase staining, alizarin red staining, and type I collagen immunohistoehemical staining, but the experimental group showed higher positive expressions than the control group (P < 0.05). The expression of osteopontin was also higher in the experimental group than the control group. These findings indicate that nucleus puplousus cells possess osteogenic ability, but have lower proliferative ability in vitro. After co-culture with periosteal cells, the proliferative ability of nucleus puplousus cells can be increased. Under osteogenic induction, nucleus puplousus cells co-cultured with periosteal cells have good compatibility and adhere with each other, which have stronger osteogenic ability than cells cultured alone.

    中国组织工程研究杂志出版内容重点:组织构建;骨细胞;软骨细胞;细胞培养;成纤维细胞;血管内皮细胞;骨质疏松组织工程

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    Differentiation and osteoprotegerin secretion of human osteoblasts: R-spondin 1 effect via Wnt/beta-catenin signal pathway  
    Wu Si-min, Liu Qing-mei, Ma Yan-yun, Wang Jiu-cun, Zhao Dong-bao
    2015, 19 (37):  5923-5927.  doi: 10.3969/j.issn.2095-4344.2015.37.004
    Abstract ( 366 )   PDF (6954KB) ( 378 )   Save

    BACKGROUND: Studies have funded that reduced Wnt/β-catenin signaling is involved in the onset and/or progression of bone erosion in rheumatoid arthritis. It can lead to potential new treatment approaches of bone erosion by enhancing Wnt/β-catenin signaling pathway. R-spondin 1 may act as a Wnt agonist, but there is no study in human osteoblasts. 

    OBJECTIVE: To verify the effect of R-spondin 1 on promoting differentiation and maturation of human osteoblasts by inhibiting DKK1.

    METHODS: S40-transfected human osteoblast lines, hFOB1.19, were treated with R-spondin 1, Wnt-3a and DKK1 to detecting the proliferation, alkaline phoshpatase activity and osteoprotegerin concentration.

    RESULTS AND CONCLUSION: R-spondin 1 had no effects on hFOB1.19 cells. Wnt-3a upregulated the activity of alkaline phoshpatase, which could be enhanced by addition of R-spondin 1. R-spondin 1 could reduce the DKK1-mediated inhibition of alkaline phoshpatase activity in hFOB1.19 cells. R-spondin 1 increased the concentration of osteoprotegerin, and moreover, the promotion of osteoprotegerin by R-spondin 1 alone was stronger than the inhibition by DKK1. These findings suggest that R-spondin 1 can inhibit DKK1 by Wnt/β-catenin signal pathway to promote the differential and maturation of human osteoblasts to excrete osteoprotegerin.

    中国组织工程研究杂志出版内容重点:组织构建;骨细胞;软骨细胞;细胞培养;成纤维细胞;血管内皮细胞;骨质疏松组织工程

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    Effects of four decalcifying solutions on pathological structure of bone and bone marrow tissues 
    Luo Can-qiao, Mo Mu-qiong, Nie Zhao-ming, Zhong Jue-min, Li Yu-bin
    2015, 19 (37):  5928-5932.  doi: 10.3969/j.issn.2095-4344.2015.37.005
    Abstract ( 794 )   PDF (4766KB) ( 697 )   Save

    BACKGROUND: As the bone and marrow tissue have very special structure, it is difficult to simultaneously display the bone with tough hard tissue and bone marrow tissues containing various immature hematopoietic cells in the conventional process of pathological section preparation.

    OBJECTIVE: To choose the best decalcifying solution that cannot only completely remove the calcium in the bone tissue but also protect the structure of bone marrow tissues and cells from damage.

    METHODS: Bone marrow tissues from the long bone of dogs were randomly divided into four groups. Under the same conditions, the bone marrow tissues were decalcified with 14% formaldehyde saline solution of nitric acid (group A), 14% nitric acid solution (group B), 20% A saline solution of hydrochloric acid formaldehyde (group C) and 20% A hydrochloric acid aqueous solution (group D). Decalcified time was recorded, followed by routine dehydration, section, hematoxylin-eosin staining and microscopic observation. Pathological section quality and hematoxylin-eosin staining were compared among the four groups.

    RESULTS AND CONCLUSION: Group A had the best sections and hematoxylin-eosin staining, strongest decalcified ability, shortest decalcified time and minimum damage to the bone marrow. Group B had the worst results of section and hematoxylin-eosin staining, in which, the bone tissues were loose and became yellow and the bone marrow tissue were damaged greatly, and the decalcified effect was worse. Group C was worse than group A in decalcified ability, damage degree, section quality and hematoxylin-eosin staining results. Group D also had a better result of section and hematoxylin-eosin staining as well as exhibited uniform decalcification effect and less damage to the bone marrow, which was ranked between group B and group C. All the four kinds of decalcifying solutions have a good decalcification ability, but the section quality and hematoxylin-eosin staining results rank as follows: Group A > Group C > Group D > Group B. Taken together, 14% formaldehyde saline solution of nitric acid is ideal for the clinical preparation of pathological sections.

    中国组织工程研究杂志出版内容重点:组织构建;骨细胞;软骨细胞;细胞培养;成纤维细胞;血管内皮细胞;骨质疏松组织工程

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    Local injection of simvastatin for repair of mild-to-moderate unstable humeral fractures: effects on bone healing and bone density
    Liu Jian, Tian Bao-fang, Wang Wen-zhu, Zheng Wei-dong, Feng Li, Du Bao-yin, Tian Da-wei
    2015, 19 (37):  5933-5937.  doi: 10.3969/j.issn.2095-4344.2015.37.006
    Abstract ( 212 )   PDF (796KB) ( 423 )   Save

    BACKGROUND: Studies have shown that a single injection of small-dose simvastatin can significantly improve bone microstructure and promote trabecular bone remodeling of the femoral condyle after osteoporosis.

    OBJECTIVE: To investigate the effect of local single injection of simvastatin on the prognosis of mild-to-moderate unstable humeral fractures.

    METHODS: A total of 93 patients with mild-to-moderate unstable humeral fracture were selected and randomized into injection group (49 cases) and normal group (44 cases). The normal group received normal physical therapy, and the injection group underwent local single injection of simvastatin based on the physical therapy. Bone mineral density and healing time were compared between two groups.

    RESULTS AND CONCLUSION: There was no difference in the bone mineral density between the two groups within 2 weeks after treatment (P > 0.05), but the bone mineral density was significantly higher in the injection group than the normal group at 1-2 months after treatment (P < 0.05). In addition, the injection group was superior to the normal group in the following aspects: incidence of complications, excellent-good rate of joint  function recovery, and hospital stay. These findings indicate that the local injection of simvastatin based on conventional physical therapy can shorten the time of fracture healing, increase bone mineral density and improve the prognosis of patients with mild-to-moderate unstable proximal humeral fractures.

    中国组织工程研究杂志出版内容重点:组织构建;骨细胞;软骨细胞;细胞培养;成纤维细胞;血管内皮细胞;骨质疏松组织工程

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    Correlation between low-energy hip fracture and knee osteoarthritis
    Zhao Wen-jie, Zhang Bin, Gao Song, Zhou Song, Wang Qiang, Dai Min
    2015, 19 (37):  5938-5942.  doi: 10.3969/j.issn.2095-4344.2015.37.007
    Abstract ( 272 )   PDF (956KB) ( 336 )   Save

    BACKGROUND: Whether the knee is a risk factor for hip fracture is controversial.

    OBJECTIVE: To investigate the effect and mechanism of knee osteoarthritis in the development of low-energy hip fracture.

    METHODS: Totally 116 patients with mild traumatic femoral neck fractures and intertrochanteric fractures admitted from October 2014 to January 2015 were enrolled and retrospectively analyzed. There were 38 males and 78 females. The mean age of onset was (69.2±14.5) years, and all the patients were diagnosed as have simple hip fractures. Anterioposterior and lateral X-ray examination for the hip and knee was performed for each patient. General information, Kellgren-Lawrence grading, scores on Hospital for Special Surgery (HSS), and Western Ontario and McMaster Universities Index of Osteoarthritis were recorded and analyzed.

    RESULTS AND CONCLUSION: Among the 116 cases, there were 66 cases of femoral neck fractures, 48 of intertrochanteric fractures and 2 of subtrochanteric femoral fractures. According to the Kellgren-Lawrence grading, there were 0 case of grade 0, 21 of grade I, 51 of grade II, 44 of grade III, and 0 of grade IV. Average Kellgren-Lawrence grading was 2.19±0.72 in fractured side and 1.51±0.52 in non-fractured side, and there was a significant difference between the fractured side and non-fractured side (P < 0.05). Average score of HSS was 78.4±8.09 in fractured side and 80.2±8.1 in non-fractured side, with statistical difference (P < 0.05). These findings indicate that knee osteoarthritis may have some adverse effects in the development of low-energy hip fracture, which can increase the incidence of femoral neck fracture and intertrochanteric fracture.

    中国组织工程研究杂志出版内容重点:组织构建;骨细胞;软骨细胞;细胞培养;成纤维细胞;血管内皮细胞;骨质疏松组织工程

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    Arthroscopic all-inside suture repair combined with sodium hyaluronate injection for discoid meniscus injury 
    Tang Jin, Ji Ming, Liao Yi-ming, Cheng Zhi-yong, Chen Lin, Zhan Fang-biao, Yang Tao
    2015, 19 (37):  5943-5949.  doi: 10.3969/j.issn.2095-4344.2015.37.008
    Abstract ( 297 )   PDF (555KB) ( 358 )   Save

    BACKGROUND: Studies have shown that arthroscopic all-inside technology for meniscus repair has achieved good outcomes, and arthroscopic suture combined with sodium hyaluronate has an important role in the cartilage repair.
    OBJECTIVE: To observe the therapeutic effect of arthroscopic all-inside suture repair combined with sodium hyaluronate injection in the repair of discoid meniscus tears.
    METHODS: Twenty-two patients with discoid meniscus injury were subjected to arthroscopic all-inside suture repair combined with sodium hyaluronate injection. All cases were confirmed to have attached edge relaxation or longitudinal crack in operation. After repair, effective rehabilitation training was done.
    RESULTS AND CONCLUSION: The operations for the 22 cases were all successful and there was no complication. All patients were followed up for over 18 months. According to Ikenchi’s method (Lysholm knee score criterion), 11 cases were rated excellent, 8 were rated good, and 3 were rated fair, with an excellent/good rate of 86.36%. It is demonstrated that the all-inside suture repair under the guide of arthroscopy in discoid meniscus cases can remain the most appropriate anatomical structure of the meniscus that can play an effective function, and has the advantage of minimal invasion. Arthroscopic all-inside suture repair combined with intra-articular injection of sodium hyaluronate and standard rehabilitation training result in a lower complication rate and effectively promotes the meniscus repair.

    中国组织工程研究杂志出版内容重点:组织构建;骨细胞;软骨细胞;细胞培养;成纤维细胞;血管内皮细胞;骨质疏松组织工程

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    Microscopic changes of freeze-dried vessels
    Xu Jie-yuan, Zhuang Mei-ping, Chen Su-fang, Lin Jing, Hong Cai-hong, Liu Meng-fang, Tao Le-ren
    2015, 19 (37):  5950-5954.  doi: 10.3969/j.issn.2095-4344.2015.37.009
    Abstract ( 313 )   PDF (1295KB) ( 426 )   Save

    BACKGROUND: Mechanical properties of the blood vessels include axial and radial tension which is important to guarantee the tissue perfusion and its pressure.
    OBJECTIVE: To test and analyze stretching and puncture parameters that can fully characterize the performance of blood vessels, so as to predict the health condition of the vessels transplanted into recipients.
    METHODS: Mechanical properties as circumferential tension, axial tension and puncture were tested on the porcine aorta, and then pathological and staining analysis was done to explore whether freeze-dried vessels can withstand the blood pressure in all directions after transplanted into the body.
    RESULTS AND CONCLUSION: Specific trend on the curves of freeze-dried vessels during circumferential tension, axial tension and puncture process was closer to that of fresh vessel curves, suggesting that structural components and arrangement of the freeze-dried vessels were not changed greatly. Meanwhile, the microscopic observations of slice and tensile section were taken into account so that the reason was studied and explained for the mechanical properties of the experimental data and results. The macroscopic mechanical result was analyzed based on the change of microstructure. These findings indicate that the vacuum freeze-drying is a better method to maintain the performance of blood vessels.

    中国组织工程研究杂志出版内容重点:组织构建;骨细胞;软骨细胞;细胞培养;成纤维细胞;血管内皮细胞;骨质疏松组织工程

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    iTRAQ technology is important to screen serum markers of differentially expressed proteins in knee osteoarthritis
    Liang Hai-bo, Su Wei, Luo Shi-xing, Dong Gui-fu, Long Guang-hua, Qin Yu-jie
    2015, 19 (37):  5955-5960.  doi: 10.3969/j.issn.2095-4344.2015.37.010
    Abstract ( 223 )   PDF (2133KB) ( 321 )   Save

    BACKGROUND: There is lack of effective diagnostic methods for early knee osteoarthritis. Proteomics refers to the large-scale experimental analysis of proteomes from the overall level of intracellular protein compositioon, expression and modification, based on which, we can understand protein-protein interaction and relationship, thereby revealing protein functions and cell activity patterns.
    OBJECTIVE: To apply the isobaric tags for relative and absolute quantitation (iTRAQ) to identify proteins differentially in serums of osteoarthritis Kellgren-Lawrence classification at each stage, and to find out potential molecular markers at each stage of osteoarthritis.
    METHODS: Sixty patients with knee osteoarthritis were enrolled, and according to Kellgren-Lawrence (K-L) grading, they were subdivided into K-L 0, II and IV groups. There were 10 males and 10 females randomly selected in each group. Proteins differentially regulated in serums were identified by the stable isotopes 116, 117, 118 of the iTRAQ labeled, reversed-phase column separation, mass spectrometry and Swissport database search, after the serums were subjected to high abundance proteins depletion. Finally, we analyzed the proteins identified by using bioinformatics software.
    RESULTS AND CONCLUSION: Totally 169 proteins were identified through iTRAQ peptides experiments of different sample tags, Q-star mass spectrum identification and MASCOT library search. 153 proteins were identified as K-L 0 to K-L IV, 153 proteins were identified as K-L 0 to K-L II, and 145 proteins were identified as K-L II to K-L IV. iTRAQ technology may help to identify novel serum markers for early diagnosis of knee osteoarthritis, indicating iTRAQ technology for proteomics serum biomarkers of osteoarthritis study has good application prospect.

    中国组织工程研究杂志出版内容重点:组织构建;骨细胞;软骨细胞;细胞培养;成纤维细胞;血管内皮细胞;骨质疏松组织工程

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    Interactions between the recombinant human CREG protein and cathepsins and M6P/IGFIIR
    Sun Ming-yu, Yan Cheng-hui, Tian Xiao-xiang, Li Yang, Han Ya-ling
    2015, 19 (37):  5961-5965.  doi: 10.3969/j.issn.2095-4344.2015.37.011
    Abstract ( 265 )   PDF (814KB) ( 326 )   Save

    BACKGROUND: It has been found that cellular repressor of E1A-stimulated genes (CREG) is a lysosomal protein binding directly to the mannose-6-phosphate (M6P)/insulin-like growth factor II receptor (IGFIIR) and depends on the interaction with M6P receptors for efficient delivery to lysosomes

    OBJECTIVE: To study the interactions between the exogenous CREG protein and cathepsins and M6P/IGFIIR and to confirm the effect of CREG protein on expression and distribution of M6P/IGFIIR.

    METHODS: Double-stained immunofluorescence and coimmunoprecipitation were applied to observe the interactions between the exogenous CREG protein and cathepsin B, cathepsin L and M6P/IGFIIR. Using gain-of-function and loss-of-function approaches, the effect of CREG on expression and distribution of 
    M6P/IGFIIR were studied by western blot assay and immunofluorescence staining.

    RESULTS AND CONCLUSION: Double-stained immunofluorescence and coimmunoprecipitation analyses confirmed the direct interactions between the exogenous CREG protein and cathepsin B, cathepsin L and M6P/IGFIIR. It was verified that CREG plays a critical role not in the expression but in the distribution of M6P/IGFIIR using gain-of-function and loss-of-function approaches. These findings provide evidence that exogenous CREG protein is located in lysosomes and has interactions with cathepsins and M6P/IGFIIR, also CREG plays a critical role in the distribution of M6P/IGFIIR.

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    Protection against apoptosis of islet cells: a combination of Caspase and Cocktail protease inhibitors
    Tian Ting-ting, Zhao Shan-shan, Wang Qun, Ye Bin
    2015, 19 (37):  5966-5971.  doi: 10.3969/j.issn.2095-4344.2015.37.012
    Abstract ( 257 )   PDF (805KB) ( 321 )   Save

    BACKGROUND: Currently, there are few reports on the effects of Caspase inhibitors combined with Cocktail protease inhibitors on isolation and purification of islet cells.
    OBJECTIVE: To explore the effects of Caspase inhibitors and Cocktail protease as protectors on islet cells during isolation and purification process.
    METHODS: New-born pigs were selected to separate, purify and culture the islet cells, and after 24 hours of culture, the specimens were divided into three groups: blank control group, experimental group A (Caspase inhibitors and Cocktail protease were added only during digestion process), and experimental group B (Caspase inhibitors and Cocktail protease were added both during digestion and culture processes). AO-EB staining was used to qualitatively observe cell morphology and apoptosis. Flow cytometry was employed to quantitatively detect cell viability and apoptosis.
    RESULTS AND CONCLUSION: Percentages of β cells were 66.91% in blank control group, 84.58% in the experimental group A, and 87.15% in the experimental group B. The proportions of living cells, apoptotic cells and 
    dead cells were 56.52%, 16.15%, 21.25% in the blank control group, 62.27%, 14.66%, 14.47% in the experimental group A, and 73.09%, 6.83%, 10.28% in the experimental group B, respectively. These findings indicate that in the process of digestion and culture in vitro, Caspase inhibitors and Cocktail protease inhibitors can both obviously decrease the loss of cells, and increase the percentage of islet beta cells.

    中国组织工程研究杂志出版内容重点:组织构建;骨细胞;软骨细胞;细胞培养;成纤维细胞;血管内皮细胞;骨质疏松组织工程

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    ABCE1 gene silencing effect on biological characteristics of human cervical cancer cells XB1702
    Li Ping, Wang De-hua
    2015, 19 (37):  5972-5977.  doi: 10.3969/j.issn.2095-4344.2015.37.013
    Abstract ( 227 )   PDF (967KB) ( 309 )   Save

    BACKGROUND: With the development of genetic engineering and tumor molecular biology, gene therapy has acted as a new treatment mode for tumors.
    OBJECTIVE: To investigate the impact of gene silencing ABCE1 on growth, proliferation and migration of human cervical cancer cells XB1702.
    METHODS: siRNA sequences of ABCE1 were designed and synthesized to transfect XB1702 cells using LipofectamineTM 2000. Cells transfected with NC siRNA carrier served as controls, and untransfected cells acted as blank group. After RNA interference, the expression of ABCE1 mRNA and protein was detected by western blod assay; cell cycle was detected using flow cytometry; cell counting kit-8 test, scratch healing assay and cell invasion assay were used to evaluate the effects of ABCE1 silence gene on XB1702 cell proliferation, migration and invasion ability.
    RESULTS AND CONCLUSION: ABCE1 mRNA and protein expression in the experimental group was significantly lower than that in the control group and blank group (P < 0.05). Growth rate in the experimental group was significantly slowed, cells were arrested in G0/G1 phase, and the number of S-phase cells was reduced. Compared with the control group and blank group, XB1702 cell proliferation was inhibited significantly in the experimental group, and cell migration and invasion was significantly decreased (P < 0.05). These results showed that specific interference with gene expression of ABCE1 can inhibit the migration of human cervical cancer cells XB1702, and restrain tumor cell proliferation; therefore, the siRNA sequence of ABCE1 may be an effective target for treatment of cervical cancer.

    中国组织工程研究杂志出版内容重点:组织构建;骨细胞;软骨细胞;细胞培养;成纤维细胞;血管内皮细胞;骨质疏松组织工程

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    Expression of neuronal cadherin and placental cadherin in facial motoneurons after facial nerve injury
    Li Lei-ji, Xu Chao-ran, Qin Gang, Liu Yue-hua, Zhu Lin
    2015, 19 (37):  5978-5982.  doi: 10.3969/j.issn.2095-4344.2015.37.014
    Abstract ( 175 )   PDF (5490KB) ( 242 )   Save

    BACKGROUND: Peripheral facial nerve injury first involves the retrograde reactions of central nervous system axons, and nerve regeneration will depend on the survival and functional status of neuronal cell bodies.

    OBJECTIVE: To explore the expression of neuronal cadherin and placental cadherin in facial nuclei after facial nerve injury.

    METHODS: New Zealand white rabbits were randomly divided into model group (n=48) and control group (n=8). In the model group, every eight rabbits were used to carry out the test respectively at 1, 4, 7, 14, 21 days after the model of facial nerve injury (right side) was established. SP and real-time quantitative PCR methods were taken to test the expression of neuronal cadherin and placental cadherin in the facial nerve nucleus at mRNA and protein levels.

    RESULTS AND CONCLUSION: The control group had neuronal cadherin- and placental cadherin-positive neurons. In the model group, neuronal cadherin and placental cadherin positively expressed in the facial motorneurons (right side), and their expressions were peaked at 14 days. Compared with the control group, the mRNA expression of neuronal cadherin in the facial motorneurons was increased significantly at 4-28 days after 
    injury; the mRNA expression of placental cadherin in the facial motorneurons was decreased significantly at 1 day after injury, and then increased significantly at 7-28 days. It is suggested that the expression of neuronal cadherin and placental cadherin is positive in the early stage of facial nerve injury, and the expression of placental cadherin is always present, while the expression of neuronal cadherin relatively lasts for a short time. After facial nerve injury, the expression of neuronal cadherin and placental cadherin in the facial nerve nucleus is both increased, which indicates that the facial nerve regeneration may be related to the high expression of adhesion molecules.

    中国组织工程研究杂志出版内容重点:组织构建;骨细胞;软骨细胞;细胞培养;成纤维细胞;血管内皮细胞;骨质疏松组织工程

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    Effect of Wudi Dan on vitality and apoptosis of cartilage chondrocytes after osteoarthritis
    Meng Ya-ke, Liu Yan, Wang Hong-rui, Liu Jian-guo, Guo Yong-fei
    2015, 19 (37):  5983-5987.  doi: 10.3969/j.issn.2095-4344.2015.37.015
    Abstract ( 200 )   PDF (6317KB) ( 318 )   Save

    BACKGROUND: Studies have found that Wudi Dan can suppress local inflammatory response of the lesioned joints to protect the articular cartilage.

    OBJECTIVE: To verify the effect of Wudi Dan on chondrocyte viability and apoptosis as well as the therapeutic effect on osteoarthritis.

    METHODS: Rat chondrocytes were cultured in serum medium containing Wudi Dan, and the effects of Wudi Dan on cell viability and apoptosis were observed by comparison with the control group. Rabbit model of knee osteoarthritis was constructed using modified Hulth method. Rabbit models were divided into two groups: Wudi Dan group treated with Wudi Dan and control group treated with normal saline, twice a day, consecutively for 4 weeks. Therapeutic effect of Wudi Dan on knee osteoarthritis was observed; cell viability and apoptosis were observed under microscope; the levels of interleukin-1 and matrix metalloproteinase-3 were determined using immunohistochemical method.

    RESULTS AND CONCLUSION: The apoptotic rate of chondrocytes was significantly lower in the Wudi Dan group than the control group. Pathological findings of the rabbit knee joints showed that the control group had more severe damage to the articular cartilage than the Wudi Dan group. Immunohistochemical staining revealed that in the Wudi Dan group, the cytoplasm and extracellular matrix were colored light and there were a small  
    number of positive cells as well as low expression of interleukin-1 and matrix metalloproteinase-3. The results suggest that Wudi Dan can effectively protect against articular cartilage lesions, reduce inflammation, and have a good therapeutic effect on osteoarthritis. Its mechanism may be related to inhibition of chondrocyte apoptosis, reduction of cytokine production and inhibition of protein expression of matrix metalloproteinase.

    中国组织工程研究杂志出版内容重点:组织构建;骨细胞;软骨细胞;细胞培养;成纤维细胞;血管内皮细胞;骨质疏松组织工程

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    Denervation effects on bone regeneration and expression of Runx2 during distraction osteogenesis 
    Zheng Ke, Song Dong-hui, Feng Xing-mei, Zhu Song-song, Hu Jing2 Ye Bin
    2015, 19 (37):  5988-5992.  doi: 10.3969/j.issn.2095-4344.2015.37.016
    Abstract ( 261 )   PDF (1709KB) ( 681 )   Save

    BACKGROUND: During the healing of fractures, removal of sciatic nerve can result in insufficient mechanical rigidity of newborn woven bone. However, there are less reports concerning the denervation effects during distraction osteogenesis.

    OBJECTIVE: To observe the effect of removal of the sciatic nerve on bone regeneration and the expression of Runt-related transcription factor 2 (Runx2) protein during distraction osteogenesis in a rabbit model.

    METHODS: Twenty-four adult male New Zealand rabbits were selected and underwent left tibial osteodistraction to construct animal models of distraction osteogenesis. Before distraction, the animals were randomly divided into group R (resecting the left sciatic nerve) and group I (intact left sciatic nerve). Six weeks after completion of distraction, the animals were killed and the lengthened tibias were harvested for radiography, three-dimensional CT reconstruction, histological evaluation, connectivity density (Conn.D) evaluation.

    RESULTS AND CONCLUSION: New regenerated bone was present and Runx2 protein was expressed in the distraction gaps of all animals at the end of the study, as revealed by radiography, three-dimensional CT reconstruction, and histological observation. However, less new bone formation and a lower degree of mineralization and expression of Runx2 protein were observed in group R compared with group I. The results suggest that the denervation appears to have an inhibitory effect on bone formation and the expression of Runx2 protein during distraction osteogenesis.

    中国组织工程研究杂志出版内容重点:组织构建;骨细胞;软骨细胞;细胞培养;成纤维细胞;血管内皮细胞;骨质疏松组织工程

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    Modified primary culture of neonatal mouse myocardial cells
    Meng Lin-lin, Huang Ying, Ma Yi-tong, Liu Fen, Chen Bang-dang, Chen Xiao-cui, Gai Min-tao
    2015, 19 (37):  5993-5997.  doi: 10.3969/j.issn.2095-4344.2015.37.017
    Abstract ( 334 )   PDF (3288KB) ( 247 )   Save

    BACKGROUND: A lot of work has been carried out on the development of the primary cultured rat myocardial cells at home and abroad. The primary culture technology of rat myocardial cells becomes more mature, but myocardial cells from neonatal mice are not easy to be obtained under the same experimental conditions. The mouse genome has more similarities with the human genome, which has a higher research value.
    OBJECTIVE: To improve the primary culture method of neonatal mouse myocardial cells, and to obtain myocardial cells with high purity, vitality and original structure and function.
    METHODS: The mouse cardiac tissues were treated using an enzyme digestion method to isolate isolated single myocardial cells: first, the cardiac tissues were digested using trypsin, and then collagenous fibers were treated with collagenase to isolate single myocardial cells. The concentration and action time of trypsin and type II collagenase were adjusted, and the pH values of reagents and temperature of each step were strictly controlled.
    RESULTS AND CONCLUSION: At 24 hours after inoculation, the myocardial cells began to be adherent; at 48 hours, independent pulsation of myocardial cells could be observed; at 72 hours, myocardial cells were cross-linked; and at 96 hours, myocardial cells formed cell clusters and presented with consistent beating. The  survival rate and purity of myocardial cells were both over 95%. This modified method could successfully culture myocardial cells with high purity and viablility from neonatal mice, and the structure and function of myocardial cells could be retained. Therefore, it is a feasible culture method.

    中国组织工程研究杂志出版内容重点:组织构建;骨细胞;软骨细胞;细胞培养;成纤维细胞;血管内皮细胞;骨质疏松组织工程

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    Hemodynamics simulation of internal carotid artery siphon and relevant influential factors
    Huang Li-dan, Deng Li-zhu, Zhao Wen-jun, Chen Li-jun, Duan Shao-yin
    2015, 19 (37):  5998-6004.  doi: 10.3969/j.issn.2095-4344.2015.37.018
    Abstract ( 385 )   PDF (2015KB) ( 314 )   Save

    BACKGROUND: The siphon of internal carotid artery is a complex of bending and surrounding structure, which has become a research hot in the field of medical imaging and regional anatomy. There is little research on the hemodynamics of internal carotid artery. Finite element analysis provides the basis for the related dynamics research.

    OBJECTIVE: To explicit the hemodynamic characteristics of the normal and stenosis internal carotid artery, and to explore the relevant influential factors.

    METHODS: Finite element model of the siphon of internal carotid artery was built based on 64-slice spiral CT data, and then, three-dimensional models were constructed using Mimics 10.01 software. There were simulations of the normal and post-stenosis blood flows with ANSYS 13.0 CFX software, to observe the changes of hemodynamics, and to analyze their characteristics and differences.

    RESULTS AND CONCLUSION: The normal blood flow at the siphon of internal carotid artery was in a laminar state, but rotation and turbulent flow formed at the two angle regions. The velocity of external bending zone was slower than that of the internal. The slower was the velocity, the more obvious was the turbulence. The wall shear  force decreased at the angle regions, and the wall shear force of external bending zone was smaller than that of the internal. At the region of arterial stenosis, the blood flow was sped, and at the downstream, turbulent flow and low zone of wall shear force were formed. With the increase of stenosis severity, the turbulent flow and low wall shear force area were expanded. Central stenosis showed more obvious effects than the eccentric one. The degree of stenosis and bending at the siphon of internal carotid artery can directly influence the formation of turbulent flow and low wall shear force area, which are more obvious at the external bending zone and central stenosis.

    中国组织工程研究杂志出版内容重点:组织构建;骨细胞;软骨细胞;细胞培养;成纤维细胞;血管内皮细胞;骨质疏松组织工程

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    Effects of Tougu Xiaotong Capsule medicated serum on the expression of uPA system and infammatory factors in osteoarthritis synovial cells 
    Wu Guang-wen, Chen Jun, Ye Jin-xia, Zheng Chun-song, Liu Bo-ling, Liu Xian-xiang, Ye Hong-zhi
    2015, 19 (37):  6005-6009.  doi: 10.3969/j.issn.2095-4344.2015.37.019
    Abstract ( 361 )   PDF (903KB) ( 253 )   Save

    BACKGROUND: Tougu Xiaotong Capsule (TGXTC) is a clinical prescription for the treatment of osteoarthritis; however, its mechanism has not been fully elucidated. Urokinase-type plasminogen activator (uPA) system 
    participating in the degradation of the extracellular matrix of articular cartilage and hyperplasia of joint synovium plays an important role in the pathological process of osteoarthritis.

    OBJECTIVE: To investigate the effects of TGXTC medicated serum on the expression of uPA, uPA receptor (uPAR), plasminogen activator inhibitors (PAIs), matrix metalloproteinase-3 (MMP-3), interleukin-1 beta (IL-1β) and tumor necrosis factor-α (TNF-α) in osteoarthritis synovial cells of rats and to discuss the mechanism by TGXTC medicated serum prevents and cures osteoarthritis.

    METHODS: Rat models with knee osteoarthritis were established by injecting 4% papain into the knee joint cavity. Primary synoviocytes and osteoarthritis synoviocytes were cultured with collagenase digestion method. The cultured synoviocytes were divided into normal group, model group and TGXTC group. The western blot method was adopted to detect uPA, uPAR, PAI, MMP-3, IL-1β and TNF-α protein expression of synoviocytes after acting by TGXTC medicated serum for 72 hours.

    ESULTS AND CONCLUSION: The expression of uPA, uPAR, MMP-3, IL-1β and TNF-α were decreased, while PAI was increased in the TGXTC group, and there were significant differences when compared with model group. In a word, TGXTC can significantly inhibit the expression of uPA, uPAR, MMP-3, IL-1β, TNF-α, and improve PAI expression in synoviocytes, which may partly explain the mechanism of the treatment of Tougu Xiaotong Capsule on osteoarthritis.

    中国组织工程研究杂志出版内容重点:组织构建;骨细胞;软骨细胞;细胞培养;成纤维细胞;血管内皮细胞;骨质疏松组织工程

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    Microtubule-associated protein-2: regulating neuronal development, structural stability, projection formation and synaptic plasticity
    Yao Zhong-kai, Wu Zuo-pei, Sun Gui-xin
    2015, 19 (37):  6010-6016.  doi: 10.3969/j.issn.2095-4344.2015.37.020
    Abstract ( 332 )   PDF (759KB) ( 357 )   Save

    BACKGROUND: Microtubule-associated protein-2 is a kind of important regulatory factor in regulating tubulin assembly. As one of the main members of microtubule-associated proteins, microtubule-associated protein-2 plays an important role in the repairing and development of the nervous system function. It has been found that microtubule-associated protein-2 can promote the repair and rebuilding of injured nerves.

    OBJECTIVE: To summarize the relationship between microtubule-associated protein-2 and nerve injury as well as the mechanism of action.

    METHODS: The PubMed database and CNKI database were retrieved by the fist author for the articles related to microtubule-associated protein-2 published from January 1976 to January 2015. The key words were “microtubule-associated protein-2 (MAP-2), nerve injury, progress” in English and Chinese, respectively. In the same field, articles published recently or in authorized journals were preferred. Repetitive or old articles were excluded, and finally 82 articles were included in result analysis.

    RESULTS AND CONCLUSION: Microtubule-associated protein-2 is involved in nerve repair, and plays a promoting role in neuronal morphology and plasticity. To increase the concentration of microtubule-associated protein-2 contributes to the recovery of neurologic function in the early stage after nerve injury.

    中国组织工程研究杂志出版内容重点:组织构建;骨细胞;软骨细胞;细胞培养;成纤维细胞;血管内皮细胞;骨质疏松组织工程

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    Non-surgical therapy for knee osteoarthritis: effectiveness evaluation using scale and biomechanical methods 
    Gang Jia-hong, Mi Yi-qun, Zhang Yong-liang, Wang Hua-min
    2015, 19 (37):  6017-6023.  doi: 10.3969/j.issn.2095-4344.2015.37.021
    Abstract ( 268 )   PDF (895KB) ( 353 )   Save

    BACKGROUND: Studies have shown that the non-surgical therapy for knee osteoarthritis is exactly effective, but there is a lack of scientific and rational evaluation system.

    OBJECTIVE: To review the evaluation methods of non-surgical therapy for knee osteoarthritis in recent 5 years.

    METHODS: A computer-based retrieval of PubMed, WOK, CNKI, VIP, Wanfang databases was performed to find literature related to the evaluation methods of non-surgical therapy for knee osteoarthritis. All data were primarily screened to exclude irrelevant literature. Those literatures about the evaluation methods of non-surgical therapy for knee osteoarthritis were included. Repetitive studies and untypical reports were excluded.

    RESULTS AND CONCLUSION: Totally 42 articles were collected, including 28 in Chinese and 14 in English. The analysis results showed that the non-surgical treatment for knee osteoarthritis can improve knee function and the quality of life in patients, and have an exact effect and a good economic benefit. Therefore, exploring a scientific and reasonable evaluation method to guide the choice of clinical treatment for knee osteoarthritis is greatly significant, which can improve the efficacy of non-surgical therapy for knee osteoarthritis. Scale-based evaluation method is simple and practical, but the presence of a single scale has a lack of objectivity. The method of biomechanics or imaging has the advantages of objective, highly reliable, accurate, non-invasive and so on. In the future therapeutic evaluation system, the combination of subjective scale observation and objective indicators should be more recommended, and the evaluation methods of non-surgical therapy for knee osteoarthritis should be selected appropriately based on the difference of clinical therapy and effect.

    中国组织工程研究杂志出版内容重点:组织构建;骨细胞;软骨细胞;细胞培养;成纤维细胞;血管内皮细胞;骨质疏松组织工程

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    Lumbar disc degeneration: tissue engineering repair and biomechanical evaluation
    Dong Shi-shi, Li Jing-feng, Zhou Yi-chi, Zha Yuan-yu, Jin Wei
    2015, 19 (37):  6024-6030.  doi: 10.3969/j.issn.2095-4344.2015.37.022
    Abstract ( 189 )   PDF (940KB) ( 311 )   Save

    BACKGROUND: At present, the clinical treatment of lumbar disc degeneration mainly includes conservative treatment, traditional surgery and minimally invasive surgery. The therapeutic purpose is to relieve symptoms, but the long-term effect is not very satisfactory. Therapeutic methods focusing on biological functional recovery have been concerned gradually, but the clinical application is far in sight.

    OBJECTIVE: To review the advances in the treatment of lumbar disc degeneration regarding tissue-engineered repair and biomechanics.

    METHODS: PubMed database was searched by the first author for relevant articles published before December 2014 using the keywords of “intervertebral disc degeneration, clinical treatment, biological treatment, tissue engineering, biomechanics, repair, progress” in English. A total of 100 articles were searched initially and finally, 40 articles were included in result analysis.

    RESULTS AND CONCLUSION: Although the therapeutic schemes are varied, the treatment of intervertebral disc degeneration is a great challenge for clinicians and basic researchers. Currently there is no perfect clinical treatment, and indications corresponding to various therapies should be paid attention as well as long-term follow-up evaluation. For various reasons, the biological treatment for intervertebral disc degenerative disease is becoming more and more popular, providing a promising prospect for the treatment of intervertebral disc degeneration. So far, large amounts of data have been obtained from animal experiments, but there are still many problems to be solved. Other challenges also involve the all aspects of general tissue engineering methods, such as cells, cytokines and scaffolds. In these studies, the nucleus pulposus tissue engineering based on the combination of heparin-functionalized chitosan hydrogel, cytokines and stem cells exhibits a promising prospect.

    中国组织工程研究杂志出版内容重点:组织构建;骨细胞;软骨细胞;细胞培养;成纤维细胞;血管内皮细胞;骨质疏松组织工程

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    Delayed onset muscle soreness and muscle satellite cells: repair of skeletal muscle injury
    Zhang Xiang, Chai Zhi-ming, Zhao Li
    2015, 19 (37):  6031-6036.  doi: 10.3969/j.issn.2095-4344.2015.37.023
    Abstract ( 282 )   PDF (903KB) ( 290 )   Save

    BACKGROUND: Skeletal muscle fiber damage that is induced by prolonged or high-intensity exercise directly cause muscle injury.
    OBJECTIVE: To propose the existence of a relationship between delayed onset muscle soreness and muscle satellite cells after skeletal muscle injury from the objective reality.
    METHODS: A retrieval of CNKI and PubMed databases was done for relevant literature published from February 1961 to February 2015 using the keywords of “delayed onset muscle soreness; skeletal muscle injury; satellite cells, skeletal muscle; myogenic regulatory factors” in Chinese and English, respectively. Finally, 59 articles were included to explore the mechanism of skeletal muscle injury accompanied by delayed onset muscle pain.
    RESULTS AND CONCLUSION: Skeletal muscle micro-injury mainly refers to ultrastructural destroy and metabolic disorders, involving muscle cell membrane, cytoskeleton, sarcomere and mitochondria, thereby leading to skeletal muscle contraction dysfunction that is often accompanied with delayed onset muscle soreness. Eccentric exercise can cause the severest microdamage to the skeletal muscle fibers. The occurrence of delayed  
    onset muscle soreness can stimulate the secretion of growth factors from skeletal muscle satellite cells to a certain extent, and the time series of delayed onset muscle soreness triggered by sustained high-intensity eccentric exercise has a certain correlation with the time series of the proliferation of skeletal muscle satellite cells.

     中国组织工程研究杂志出版内容重点:组织构建;骨细胞;软骨细胞;细胞培养;成纤维细胞;血管内皮细胞;骨质疏松组织工程

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    Articular injection of opioids for postoperative pain management of knee arthroplasty: current status and progress
    Zhang Miao, Zhang Xu-ran, Sun Yu
    2015, 19 (37):  6037-6041.  doi: 10.3969/j.issn.2095-4344.2015.37.024
    Abstract ( 217 )   PDF (451KB) ( 250 )   Save

    BACKGROUND: Articular injections of opioids are widely adopted for pain management of total knee arthroplasty, with both peri-articular and intra-articular administration routes. Recent studies have indicated that commonly used anesthetics, steroids and non-steroidal anti-inflammatory drugs are associated with potential adverse effects. Peripheral opioids are relatively safe with lower complication rates, but great controversy exits regarding the analgesic effects.

    OBJECTIVE: To introduce the current clinical application status and basic research progress in peri-articular and intra-articular opioid injections for postoperative analgesia of knee arthroplasties.

    METHODS: A computer-based search in PubMed and Embase databases was conducted for clinical and basic research articles related to pain management of knee arthroplasties using peri-articular and intra-articular injections of opioids published from January 1967 to May 2015, using the keywords of “opioids; peri-articular injection; intra-articular injection; analgesia; knee arthroplasty” in English. Irrelevant, poorly related and repetitive studies were excluded.

    RESULTS AND CONCLUSION: Peri-articular and intra-articular opioids injection plans vary greatly between different institutions, which does not support reliable quantitative data synthesis for a meta-analysis. Controversy exists regarding its efficacy, but there are studies supporting the usage of opioids for providing effective analgesia in a dose-dependent manner. The peri-articular administration route is associated with lower complication rates including nausea and vomiting compared to systematic injections. Applications of opioids help to avoid potential chondrocyte and stem cell cytotoxicity caused by other anesthetics, steroids and non-steroidal anti-inflammatory drugs, which is important for pain management of total knee arthroplasties. Future investigations are required to promote articular analgesic effects and time duration by exploring more advanced drug combinations and dosage forms.

     中国组织工程研究杂志出版内容重点:组织构建;骨细胞;软骨细胞;细胞培养;成纤维细胞;血管内皮细胞;骨质疏松组织工程

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    Rehabilitation practice and outcomes after spinal cord injury 
    Wang Yu-ming, Wu Yong-gang, Duan Li-jun
    2015, 19 (37):  6042-6047.  doi: 10.3969/j.issn.2095-4344.2015.37.025
    Abstract ( 307 )   PDF (822KB) ( 310 )   Save

    BACKGROUND: Rehabilitation outcomes of patients with spinal cord injury are associated with degree of injury, therapeutic method, recovery time and subsequent treatment. Multidisciplinary, comprehensive, specialized rehabilitation unit can provide a better recovery after spinal cord injury.

    OBJECTIVE: To review the effect or combined effect of rehabilitation units after spinal cord injury.

    METHODS: A computer-based search of Springer and PubMed databases was done using the keywords of “spinal cord injury, rehabilitation practice, outcomes”, which appeared in the title and abstract. Finally, 44 English papers were included in result analysis.

    RESULTS AND CONCLUSION: Based on practical evidence, various rehabilitation practices are recognized, and then, the relevant information is connected with the outcomes to evaluate the efficacy of rehabilitation interventions. Studies have shown that age had no influence on rehabilitation outcomes in traumatic and non-traumatic mixed samples, and there is also little difference in the rehabilitation outcomes between male and female. Incidence of complications is lowest in the vast majority of patients with spinal cord injury who are initially admitted to a specialist center of spinal cord injury. The hospital stay can be shorten in patients who can be admitted to a multidisciplinary, comprehensive, specialized division of spinal cord injury as early as possible. Patients who can receive regular, comprehensive outpatient follow-up show no significant differences in health perception, independence and depression, but the frequency and degree of certain secondary situations can be significantly reduced.
     

     中国组织工程研究杂志出版内容重点:组织构建;骨细胞;软骨细胞;细胞培养;成纤维细胞;血管内皮细胞;骨质疏松组织工程

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    Biological function and exercise regulation of high-density lipoprotein
    Su Zhong-jun, Zhang Su, Jiang Jun
    2015, 19 (37):  6048-6054.  doi: 10.3969/j.issn.2095-4344.2015.37.026
    Abstract ( 210 )   PDF (903KB) ( 329 )   Save

    BACKGROUND: High-density lipoprotein is related to lipid metabolism, but its function cannot be restricted to the scope of lipid metabolism. Simultaneously, exercise regulation has effects on the activity and amount of high-density lipoprotein, but the optimal movement pattern needs to be studied.

    OBJECTIVE: To offer the theoretical support for formulating fitness training and prospect the possible direction and reference in follow-up studying the biological function and exercise regulation of high-density lipoprotein.

    METHODS: PubMed database was searched for relevant articles published from 1990 to 2015 using the keywords of “HDL and exercise, HDL and biological function” in English. Finally, 44 articles were included in result analysis.

    RESULTS AND CONCLUSION: The biological functions of high-density lipoprotein mainly include high-density lipoprotein-mediated metabolism of glucose, protein and fat, anti-inflammation, antioxidation and insulin secretion, and at the same time, we summarize the effects of exercise on the concentration, numbers of molecular and components of high-density lipoprotein. The results indicate that the combination of resistance and endurance training is the best way for raising the concentration and functional effects of high-density lipoprotein.

     中国组织工程研究杂志出版内容重点:组织构建;骨细胞;软骨细胞;细胞培养;成纤维细胞;血管内皮细胞;骨质疏松组织工程

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    A survey on the incidence of hip fractures in middle-aged and old population from Changning District, Shanghai 
    Zhang Guo-ning, Hou Bo, Shan Lian-cheng, Wang Jia, Yang Wei, Liang Chao-ge
    2015, 19 (37):  6055-6059.  doi: 10.3969/j.issn.2095-4344.2015.37.027
    Abstract ( 279 )   PDF (637KB) ( 596 )   Save

    BACKGROUND: Hip fracture has become one of the main reasons for morbidity, death and heavy medical costs in the elderly. With aging, the incidence of hip fractures in the elderly is increased day by day.

    OBJECTIVE: To investigate and analyze the incidence and distribution of hip fractures in middle-aged and old population from Changning District of Shanghai, China.

    METHODS: Medical records of patients with hip fracture ICD-9 CODE (femoral neck fracture and intertrochanteric fracture) admitted from July 2008 to June 2013 were collected at the Changning District Public Health Information Center and Shanghai Shen Kang Medical Alliance Center. Population distributions of Changning District in 2010 and 2011 were provided by the Disease Control Center of Changning District. Then, the crude morbidity of hip fracture was calculated in terms of different ages and genders. For patients aged over 50 years, every 5 years served as an age group.

    RESULTS AND CONCLUSION: The incidence rate of hip fractures in the population aged over 50 years was 197.04/100 000, 88.51/100 000 in male and 304.73/100 000 in female. The annual standardized incidence rate of hip fracture was higher in female than male (P < 0.05). The crude incidence rate of hip fracture was increased dramatically in the population over 70 years, especially in females. It indicates that the incidence rate of hip fractures is closed related to age, gender and osteoporosis.

     中国组织工程研究杂志出版内容重点:组织构建;骨细胞;软骨细胞;细胞培养;成纤维细胞;血管内皮细胞;骨质疏松组织工程

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    Reliability of pressure biofeedback instrument in patients with low back pain
    Dong Yu-lin, Wang Xue-qiang, Hu Jing-yun1
    2015, 19 (37):  6060-6063.  doi: 10.3969/j.issn.2095-4344.2015.37.028
    Abstract ( 463 )   PDF (696KB) ( 673 )   Save

    BACKGROUND: Treatment programs for low back pain are of great varieties, but there is still no a definite treatment. Pressure biofeedback instrument is a tool to measure abdominal muscle activity, and its reliability in the treatment of low back pain is less reported.

    OBJECTIVE: To explore the reliability and feasibility of the pressure biofeedback instrument for assessment of low back pain.

    METHODS: Thirty patients with chronic low back pain were enrolled and subject to four kinds of postures: posture A was prone position; posture B was supine position with flexion of the knee joints, posture C was supine position with unilateral flexion of the hip and knee joint, posture D was prone position with unilateral shoulder flexion. Intra-abdominal pressure values under the different postures were measured twice within a week in the same subject. Intraclass correlation coefficient (ICC) was used to assess the reliability.

    RESULTS AND CONCLUSION: Posture A had good test-retest reliability, and the value of ICC was 0.853 (95% confidence interval: 0.691-0.930); posture B showed moderate test-retest reliability, and the ICC value was 0.751 (95% confidence interval: 0.477-0.882); posture C also had moderate test-retest reliability, and the ICC value was 0.789 (95% confidence interval: 0.557-0.900), posture D had good test-retest reliability, and the value of ICC was 0.892 (95% confidence interval: 0.641-0.919). Therefore, the pressure biofeedback for evaluation of low back pain has good reliability, which can be used for the evaluation of low back pain.

     中国组织工程研究杂志出版内容重点:组织构建;骨细胞;软骨细胞;细胞培养;成纤维细胞;血管内皮细胞;骨质疏松组织工程

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    Association between IRF6 gene and non-syndromic cleft lip and palate in Xinjiang Uygur population: a next-generation sequencing analysis 
    Gu Li, Bilikezi Yusufu, Ainiwaer Mijiti, Adili Moming
    2015, 19 (37):  6064-6068.  doi: 10.3969/j.issn.2095-4344.2015.37.029
    Abstract ( 281 )   PDF (763KB) ( 372 )   Save

    BACKGROUND: There are few genetic data related to nonsyndromic cleft lip and palate in Xinjiang Uygur population.

    OBJECTIVE: To investigate the relationship between interferon regulatory factor 6 (IRF6) and non-syndromic cleft lip and palate in Xinjiang Uygur population.

    METHODS: A total of 100 Uyghur patients with non-syndromic cleft lip and palate from Xinjiang were included as case group, and meanwhile, 60 children with upper respiratory infection were enrolled as controls. Next-generation sequencing was used to detect coding region, 5’UTR and 500 bp before exon1 of IRF6 gene, and sequencing results were compared with the information on the genome database.

    RESULTS AND CONCLUSION: Eleven high-frequency SNPs were found: rs861019, rs7552506, rs2235377, 
    rs2235371, rs2013162, rs7545538, rs7545542, rs12403006, rs846808, rs34743335, rs2235373. The frequencies of alleles of loci rs7545538 (C > G, P=0.007), rs7545542 (C > T, P=0.044) and rs2235373 (G > A, P=0.049) were significantly different in the case group and control group; the genotype distribution of rs7545538 showed significant difference between the case group and control group (P=0.037); the genotype distribution and the frequency of alleles of other loci had no significant difference between the case group and control group (P > 0.05). Two blocks were identified in the 11 high-frequency SNPs. There were four common haploid types in Block 1: CCGGT > CCGAT > CACAT > TAGAC, and the most significant single type was CCGAT (P=0.032). There were three common haploid types in Block 2: TAC > ATG > TAG, and the most significant single types were TAC (P=0.009) and TAG (P=0.003). These findings indicate that IRF6 gene polymorphisms are probably associated with the occurrence of non-syndromic cleft lip and palate in Xinjiang Uygur population.

     中国组织工程研究杂志出版内容重点:组织构建;骨细胞;软骨细胞;细胞培养;成纤维细胞;血管内皮细胞;骨质疏松组织工程

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