Effect of Wudi Dan on vitality and apoptosis of cartilage chondrocytes after osteoarthritis

doi:10.3969/j.issn.2095-4344.2015.37.015

Abstract

Abstract:

BACKGROUND: Studies have found that Wudi Dan can suppress local inflammatory response of the lesioned joints to protect the articular cartilage.

OBJECTIVE: To verify the effect of Wudi Dan on chondrocyte viability and apoptosis as well as the therapeutic effect on osteoarthritis.

METHODS: Rat chondrocytes were cultured in serum medium containing Wudi Dan, and the effects of Wudi Dan on cell viability and apoptosis were observed by comparison with the control group. Rabbit model of knee osteoarthritis was constructed using modified Hulth method. Rabbit models were divided into two groups: Wudi Dan group treated with Wudi Dan and control group treated with normal saline, twice a day, consecutively for 4 weeks. Therapeutic effect of Wudi Dan on knee osteoarthritis was observed; cell viability and apoptosis were observed under microscope; the levels of interleukin-1 and matrix metalloproteinase-3 were determined using immunohistochemical method.

RESULTS AND CONCLUSION: The apoptotic rate of chondrocytes was significantly lower in the Wudi Dan group than the control group. Pathological findings of the rabbit knee joints showed that the control group had more severe damage to the articular cartilage than the Wudi Dan group. Immunohistochemical staining revealed that in the Wudi Dan group, the cytoplasm and extracellular matrix were colored light and there were a small
number of positive cells as well as low expression of interleukin-1 and matrix metalloproteinase-3. The results suggest that Wudi Dan can effectively protect against articular cartilage lesions, reduce inflammation, and have a good therapeutic effect on osteoarthritis. Its mechanism may be related to inhibition of chondrocyte apoptosis, reduction of cytokine production and inhibition of protein expression of matrix metalloproteinase.

中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程

Key words: Osteoarthritis, Drugs, Chinese Herbal, Chondrocytes

CLC Number:

R318

Meng Ya-ke, Liu Yan, Wang Hong-rui, Liu Jian-guo, Guo Yong-fei. Effect of Wudi Dan on vitality and apoptosis of cartilage chondrocytes after osteoarthritis[J]. Chinese Journal of Tissue Engineering Research, 2015, 19(37): 5983-5987.

Figures/Tables 1

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