Differentiation and osteoprotegerin secretion of human osteoblasts: R-spondin 1 effect via Wnt/beta-catenin signal pathway

doi:10.3969/j.issn.2095-4344.2015.37.004

Abstract

Abstract:

BACKGROUND: Studies have funded that reduced Wnt/β-catenin signaling is involved in the onset and/or progression of bone erosion in rheumatoid arthritis. It can lead to potential new treatment approaches of bone erosion by enhancing Wnt/β-catenin signaling pathway. R-spondin 1 may act as a Wnt agonist, but there is no study in human osteoblasts.

OBJECTIVE: To verify the effect of R-spondin 1 on promoting differentiation and maturation of human osteoblasts by inhibiting DKK1.

METHODS: S40-transfected human osteoblast lines, hFOB1.19, were treated with R-spondin 1, Wnt-3a and DKK1 to detecting the proliferation, alkaline phoshpatase activity and osteoprotegerin concentration.

RESULTS AND CONCLUSION: R-spondin 1 had no effects on hFOB1.19 cells. Wnt-3a upregulated the activity of alkaline phoshpatase, which could be enhanced by addition of R-spondin 1. R-spondin 1 could reduce the DKK1-mediated inhibition of alkaline phoshpatase activity in hFOB1.19 cells. R-spondin 1 increased the concentration of osteoprotegerin, and moreover, the promotion of osteoprotegerin by R-spondin 1 alone was stronger than the inhibition by DKK1. These findings suggest that R-spondin 1 can inhibit DKK1 by Wnt/β-catenin signal pathway to promote the differential and maturation of human osteoblasts to excrete osteoprotegerin.

中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程

Key words: Osteoprotegerin, Osteoblasts, Osteoclasts

CLC Number:

R318

Wu Si-min, Liu Qing-mei, Ma Yan-yun, Wang Jiu-cun, Zhao Dong-bao. Differentiation and osteoprotegerin secretion of human osteoblasts: R-spondin 1 effect via Wnt/beta-catenin signal pathway [J]. Chinese Journal of Tissue Engineering Research, 2015, 19(37): 5923-5927.

Figures/Tables 4

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