Interactions between the recombinant human CREG protein and cathepsins and M6P/IGFIIR

doi:10.3969/j.issn.2095-4344.2015.37.011

Chinese Journal of Tissue Engineering Research ›› 2015, Vol. 19 ›› Issue (37): 5961-5965.doi: 10.3969/j.issn.2095-4344.2015.37.011

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Interactions between the recombinant human CREG protein and cathepsins and M6P/IGFIIR

Sun Ming-yu, Yan Cheng-hui, Tian Xiao-xiang, Li Yang, Han Ya-ling

Department of Cardiology, Cardiovascular Research Institute of PLA, General Hospital of Shenyang Military Region, Shenyang 110016, Liaoning Province, China

Online:2015-09-10 Published:2015-09-10
Contact: Han Ya-ling, M.D., Academician, Professor, Doctoral supervisor, Department of Cardiology, Cardiovascular Research Institute of PLA, General Hospital of Shenyang Military Region, Shenyang 110016, Liaoning Province, China
About author:Sun Ming-yu, M.D., Associate chief physician, Department of Cardiology, Cardiovascular Research Institute of PLA, General Hospital of Shenyang Military Region, Shenyang 110016, Liaoning Province, China
Supported by:
the National Natural Science Foundation of China, No. 81130072, 81370243

Abstract

Abstract:

BACKGROUND: It has been found that cellular repressor of E1A-stimulated genes (CREG) is a lysosomal protein binding directly to the mannose-6-phosphate (M6P)/insulin-like growth factor II receptor (IGFIIR) and depends on the interaction with M6P receptors for efficient delivery to lysosomes

OBJECTIVE: To study the interactions between the exogenous CREG protein and cathepsins and M6P/IGFIIR and to confirm the effect of CREG protein on expression and distribution of M6P/IGFIIR.

METHODS: Double-stained immunofluorescence and coimmunoprecipitation were applied to observe the interactions between the exogenous CREG protein and cathepsin B, cathepsin L and M6P/IGFIIR. Using gain-of-function and loss-of-function approaches, the effect of CREG on expression and distribution of
M6P/IGFIIR were studied by western blot assay and immunofluorescence staining.

RESULTS AND CONCLUSION: Double-stained immunofluorescence and coimmunoprecipitation analyses confirmed the direct interactions between the exogenous CREG protein and cathepsin B, cathepsin L and M6P/IGFIIR. It was verified that CREG plays a critical role not in the expression but in the distribution of M6P/IGFIIR using gain-of-function and loss-of-function approaches. These findings provide evidence that exogenous CREG protein is located in lysosomes and has interactions with cathepsins and M6P/IGFIIR, also CREG plays a critical role in the distribution of M6P/IGFIIR.

Key words: 组织构建, 组织工程, 巨噬细胞, E1A激活基因阻遏子, 溶酶体, 动脉粥样硬化, 国家自然科学基金

CLC Number:

R318

Sun Ming-yu, Yan Cheng-hui, Tian Xiao-xiang, Li Yang, Han Ya-ling. Interactions between the recombinant human CREG protein and cathepsins and M6P/IGFIIR[J]. Chinese Journal of Tissue Engineering Research, 2015, 19(37): 5961-5965.

Figures/Tables 4

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Interactions between the recombinant human CREG protein and cathepsins and M6P/IGFIIR

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