Expression of hypoxia-inducible factor 1 alpha and its relationship to apoptosis in human lumbar nucleus pulposus

doi:10.3969/j.issn.2095-4344.2015.33.007

Abstract

Abstract:

BACKGROUND: Under hypoxic environment, hypoxia-inducible factor 1α plays a dual regulatory role in cell apoptosis. Severity of hypoxia is the key to determine whether cells appear to have apoptosis or adapt to survive. When the cells are exposed to chronic or extreme hypoxia, a lack of protection mechanisms from hypoxia-inducible factor-1α can induce cell apoptosis.
OBJECTIVE: To research the expression of hypoxia-inducible factor 1α in human lumbar nucleus pulposus of different herniated types and its relationships with cell apoptosis.
METHODS: The nucleus pulposus was harvested from 60 cases of herniation of lumbar intervertebral discs, L4-5 in 41 cases and L5-S1 in 19 cases. The nucleus pulposus tissues were equally divided into protruded and sequestered groups. Meanwhile, the nucleus pulposus tissues from another 10 cases of lumbar spine fracture were taken as control group. Expression of hypoxia-inducible factor 1α and apoptosis of lumbar nucleus pulposus cells were observed and detected with immunohistochemical technique and TUNEL method. Correlation of hypoxia-inducible factor 1α and apoptosis in human lumbar nucleus pulposus of different herniated types was analyzed.
RESULTS AND CONCLUSION: The expression of hypoxia-inducible factor 1α was visualized in each case, but it was significantly higher in the sequestered group than in the protruded group and control group (P < 0.01). Apoptosis of nucleus pulposus cells were found in all the three groups, but the apoptotic rate was also higher in the sequestered group than in the protruded group and control group (P < 0.01). Expression of hypoxia-inducible factor 1α was positively correlated with cell apoptosis in human lumbar nucleus pulposus (P < 0.01). Overall, the expression of hypoxia-inducible factor 1α in degenerative human lumbar nucleus pulposus is associated with herniated types, which is the highest in the sequestered type. The relationship between hypoxia-inducible factor 1α and apoptosis is positive.

中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程

Key words: 组织构建, 组织工程, 髓核, 腰椎, 缺氧诱导因子1α, 凋亡

CLC Number:

R318

Liu Xing-zhen, Jin Wen-jie, Shen Kang-ping, Fu Zhi-yi, Wu Yu-jie. Expression of hypoxia-inducible factor 1 alpha and its relationship to apoptosis in human lumbar nucleus pulposus[J]. Chinese Journal of Tissue Engineering Research, 2015, 19(33): 5279-5283.

Figures/Tables 1

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