Neuroprotective effects of salvianolic acid B against Schwann cell injury induced by lipopolysaccharide

doi:10.3969/j.issn.2095-4344.2015.42.017

Abstract

Abstract:

BACKGROUND: Salvianolic acid B is an effective monomer component of Salvia miltiorrhiza, which has been shown in recent years to have neuroprotective role and to promote nerve recovery, but its mechanism is not clear.

OBJECTIVE: To explore the protective mechanism of salvianolic acid B on Schwann cells in rats with spinal cord injury.

METHODS: Schwann cells of Sprague-Dawley rats were cultured and divided into normal control group, model group, 10 μmol/L methylprednisolone group and salvianolic acid B group (0.1, 1, 10, 100 μmol/L). Models of Schwann cell injury induced by lipopolysaccharide were established in all the groups except the normal control group. After intervention, growth curve and proliferative activity of Schwann cells were detected, and protein and gene expressions of β-catenin and nuclear factor-κB were observed.

RESULTS AND CONCLUSION: At 48, 72, 96 hours after intervention, the cell viability of the model group was significantly lower than that of the normal control group (P < 0.01). Compared with the model group, the cell viability of salvianolic acid B group (10 μmol/L) was significantly increased at 72 and 96 hours (P < 0.05); the expressions of nuclear factor-κB protein and mRNA in the methylprednisolone group and salvianolic acid B group were declined significantly (P < 0.01), but the expressions of β-catenin mRNA and protein in the salvianolic acid B group significantly increased (P < 0.05). These results suggest that salvianolic acid B improves the viability of Schwann cells which are stimulated with lipopolysaccharide, suppresses expression of nuclear factor-κB mRNA and protein, and promotes the expression of β-catenin mRNA and protein. Above may be one of the mechanisms which salvianolic acid B protects Schwann cells.
中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程

Key words: 组织构建, 骨组织工程, 脊髓损伤, 许旺细胞, 丹参, 丹酚酸B, 神经修复, 脂多糖, 核因子κB, Wnt/β-catenin, β-catenin, 中药单体

Bi Lian-yong. Neuroprotective effects of salvianolic acid B against Schwann cell injury induced by lipopolysaccharide[J]. Chinese Journal of Tissue Engineering Research, 2015, 19(42): 6813-6818.

Figures/Tables 5

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