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    08 October 2015, Volume 19 Issue 42 Previous Issue    Next Issue
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    Effects of eucommia alcohol extract on a rabbit model of mandibular distraction osteogenesis
    Jiang Xiao-wen, Zhang Yi, Fan Xiao-sheng, Deng Xuan
    2015, 19 (42):  6725-6729.  doi: 10.3969/j.issn.2095-4344.2015.42.001
    Abstract ( 326 )   PDF (1202KB) ( 330 )   Save

    BACKGROUND: Long course of treatment and complications limit the extensive application of distraction osteogenesis, which cannot meet the clinical requirements.
    OBJECTIVE: To investigate the effect of general administration of eucommia alcohol extract on new bone regeneration based on the rabbit model of mandibular distraction osteogenesis.
    METHODS: Twenty-four New Zealand white rabbits were randomly divided into experimental group and control group. Unilateral mandibular distraction osteogenesis model was established by 1 mm/12 h distraction protocol. During the distraction period, the rabbits in the experimental group were intragastrically administered with eucommia alcohol extract and the control animals received the same amount of physiological saline, respectively. Six weeks later, the animals were sacrificed for osteogenic testing.
    RESULTS AND CONCLUSION: New bone formation was observed in the distracted gap in both groups. However, the amount, mineralization and biomechanical strength of new regenerated bone in the experimental group were obviously greater than those in the control group by histological observation, dual energy X-ray 
    absorptiometry, micro-CT and biomechanical test. General administration of eucommia alcohol extract can markedly promote distraction osteogensis in rabbit mandibular osteodistraction.

    中国组织工程研究杂志出版内容重点:组织构建;骨细胞;软骨细胞;细胞培养;成纤维细胞;血管内皮细胞;骨质疏松组织工程

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    Establishing a finite element model of the mandible containing the temporomandibular joint after bilateral-sagitta-split-ramus-osteotomy with internal fixation
    Ma Wen, Hou Min, Song Da-li, Yang Jing-wen, Dai Zhi, Cheng Jia-long, Chai Guo-liang, Zhou Wei-yuan, Zhang Rui-ze
    2015, 19 (42):  6730-6734.  doi: 10.3969/j.issn.2095-4344.2015.42.002
    Abstract ( 414 )   PDF (1487KB) ( 354 )   Save

    BACKGROUND: Bilateral-sagitta-split-ramus-osteotomy (BSSRO) has become a conventional method to correct facial deformities, and the finite element method is a significant way to study biomechanics of the mandible and temporomandibular joint (TMJ) after BSSRO.

    OBJECTIVE: To establish a precise and high simulation model of mandible containing TMJ after BSSRO with internal fixation, which is the base to study the biomechanics of the mandible and TMJ after BSSRO.
    METHODS: Spiral CT scan was used to get the data of DICOM that were input into MIMICS to establish the three-dimensional model of the mandible. The three-dimensional model was wrapped into a single closed shell for mesh generation and conversion in ANSYS. Then, the model was input into the ANSYS software for temporomandibular joint reconstruction and simulation of BSSRO and internal fixation.
    RESULTS AND CONCLUSION: The three-dimensional finite element model of mandible containing TMJ after BSSRO was established using MIMICS and ANSYS. This model had biological similarity and geometric similarity in comparison with the human tissues. The model could undergo various internal fixations through antedisplacement, retroposition and rotational movement of the distal end. Based on different experimental purposes, the established model can apply a load to all parts to study changes in stress and displacement of different tissues after BSSRO and internal fixation, and it also can be used to study the effect of different fixation materials on the rear stability after internal fixation.
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    Calcitonin effect on cartilage morphology and proteoglycan expression in rats with osteoarthritis
    Tao Ye, Zhao Xin-you, Fang Guang
    2015, 19 (42):  6735-6739.  doi: 10.3969/j.issn.2095-4344.2015.42.003
    Abstract ( 256 )   PDF (4858KB) ( 681 )   Save

    BACKGROUND: In recent years, calcitonin has been reported to have better clinical efficacy in the treatment of osteoarthritis, but its mechanism of action for osteoarthritis is rarely reported.

    OBJECTIVE: To investigate the effect of calcitonin on the cartilage morphology and proteoglycan expression in rats with osteoarthritis.
    METHODS: Thirty Sprague Dawley rats were randomly divided into three groups: model group, treatment group, and sham group. Anterior cruciate ligament transaction of the right limbs was implemented in the model and treatment groups, and only the joint cavity was exposed in the sham group. At 2 days after modeling, the treatment group received a daily subcutaneous injection of salmon calcitonin, 15 IU/(kg•d), and the model group and sham group were administered with normal saline at the same dose. The injection lasted for 6 weeks. At 10 weeks after modeling, the articular surface of the tibia of rats in each group was generally observed; bone mineral density of the distal femoral bone and subchondral bone of the lateral and medial ankle were detected using X-ray test; bone morphology and proteoglycan secretion were measured by hematoxylin-eosin staining and toluidine blue staining, respectively.
    RESULTS AND CONCLUSION: The tibial articular surface was smooth and glossy in the sham group, but oxblood in the model group with large-area ulcers; the treatment group showed rough and local ulceration. Compared with the sham group, the bone mineral density of the subchondral bone of medial and lateral ankle were increased significantly in the model group (P < 0.05), but trabecular separation and proteoglycan content were decreased (P < 0.05). Compared with the model group, the bone mineral density of the subchondral bone of medial and lateral ankle were decreased significantly in the treatment group (P < 0.05), while the trabecular separation and proteoglycan content were increased (P < 0.05). These findings indicate that calcitonin has better protection on the cartilage of rats with osteoarthritis, and can promote bone the secretion of proteoglycan.
    中国组织工程研究杂志出版内容重点:组织构建;骨细胞;软骨细胞;细胞培养;成纤维细胞;血管内皮细胞;骨质疏松组织工程
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    Denervation of the inferior alveolar nerve is associated with bone formation and bone mass maintenance in extraction sockets
    Wang Cheng-yong, Chen Wei-hui, Lu Meng, Wang Jin, Mao Chuan-qing, Lin Hai
    2015, 19 (42):  6740-6745.  doi: 10.3969/j.issn.2095-4344.2015.42.004
    Abstract ( 384 )   PDF (4870KB) ( 517 )   Save

    BACKGROUND: There is a close relationship between innervations and bone formation and bone mass maintenance in the extraction sockets.

    OBJECTIVE: To study the possible effect of denervations on the regulation of new bone formation and bone mass maintenance in the extraction sockets.
    METHODS: The unilateral inferior nerve of dogs was sectioned to establish an animal denervation model. The normal side was used as control. After model establishment, the premolars of denervated side and normal side were extracted. Histological method was used to test new bone formation and bone mass maintenance in the extraction sockets at the 2nd, 4th, 8th and 12th weeks after tooth extraction.

    RESULTS AND CONCLUSION: The percentage of new bone areas in the extraction sockets was significantly lower in the experiment group than the control group at weeks 2, 4, 8 after tooth extraction (P < 0.01). The height difference between the buccal and lingual alveolar ridge was higher in the experimental group than the control group at weeks 2, 4, 8, 12 after tooth extraction (P < 0.05 or P < 0.01). These findings indicate that denervation is closely related with new bone formation and bone mass maintenance in the extraction sockets.
    中国组织工程研究杂志出版内容重点:组织构建;骨细胞;软骨细胞;细胞培养;成纤维细胞;血管内皮细胞;骨质疏松组织工程

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    Repairing skin defects in nude mice using tissue-engineered skin loading keratinocyte growth factor nanocapsules
    Li Bing-hang, Deng Li-huan, Xiang Meng-juan, Yang Bin
    2015, 19 (42):  6746-6752.  doi: 10.3969/j.issn.2095-4344.2015.42.005
    Abstract ( 256 )   PDF (4312KB) ( 535 )   Save

    BACKGROUND: As an emerging technology, tissue-engineered skin has great application prospects. Keratinocyte growth factor (KGF) is proved to promote the proliferation of epidermal cells.
    OBJECTIVE: To evaluate the effect and characteristics of tissue-engineered skin carrying KGF nanocapsules in repairing skin defects of nude mice.
    METHODS: (1) The acellular dermal matrix loading KGF (KGF-ADM) was constructed. The human epidermal stem cell population and fibroblasts were captured and cultivated, and then identified. Epidermal stem cells were cultivated on the KGF-ADM and their growth was observed. The tissue-engineered skin loading KGF nanocapsules was transplanted onto the full-skin defects on the back of nude mice compared with a blank group without keratinocyte growth factor nanocapsules and a control group with skin autograft. In 2, 4 and 6 weeks after transplantation, the contraction and histological healing of the skin were observed respectively. Then anti-human keratin 10-FITC and β1-integrin-Cy3 immunofluorescence were applied to detect the origin, growth and differentiation of stem cells in the epidermis and dermis.
    RESULTS AND CONCLUSION: The epidermal stem cell population grew well on the surface of KGF-ADM and attached tightly. There were small round epidermal stem cells and polygonal terminally-differentiated cells, which presented with partly cloning growth and a tendency of merging into pieces. The results of tissue-engineered skin with KGF nanocapsules in repairing the skin defects were better than those of the blank group and the control group in 2, 4 and 6 weeks after transplantation. The transplanted skin could fuse with adjacent skin completely, but still showed some contraction. Under the microscope, they showed good epidermis with layers and normal keratose stratum, and meanwhile, there were still some β1-integrin+ cells at 8 and 10 weeks, which were epidermal stem cells or transient amplifying cells identified by immunofluorescence. These findings indicate that the tissue-engineered skin carrying KGF nanocapsules has good outcomes in repairing skin defects of nude mice, which is better than common tissue-engineered skin without KGF nanocapsules and autogeneous skin transplantation.
    中国组织工程研究杂志出版内容重点:组织构建;骨细胞;软骨细胞;细胞培养;成纤维细胞;血管内皮细胞;骨质疏松组织工程

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    The normal measurements of the hyoid bone position in populations aged 6-19 years from Shandong using cone-beam CT
    Jiang Ying-ying, Xu Xin, Hu Wen-ting
    2015, 19 (42):  6753-6758.  doi: 10.3969/j.issn.2095-4344.2015.42.006
    Abstract ( 375 )   PDF (1105KB) ( 350 )   Save

    BACKGROUND: With the extensive application of cone-beam CT in oral and craniofacial surgery, a clear hyoid bone position indicator for normal population has important implications for the change of hyoid bone position before and after orthodontics.

    OBJECTIVE: To determine the cone-beam CT measurement range of hyoid bone position in populations aged 6-19 years from Shandong, so as to provide a reference marker for the change of hyoid bone position before and after orthodontics in local children and adolescents.
    METHODS: Totally 254 healthy children (120 males and 134 females) aged 6-19 years from Shandong Province were subjected to cone-beam CT scan of the hyoid bone. Mimics10.01 was used to evaluate the linear and angular measurements of hyoid bone position, and then the normal value range was confirmed. Independent-sample t-test was used for analysis of gender difference and 95% confidence interval was calculated.
    RESULTS AND CONCLUSION: There were gender differences in a part of indicators of hyoid bone position among 14-15 years, 16-17 years, 18-19 years groups (P < 0.05). For children aged 12-13 years, the hyoid bone position vertical to the base of skull and upper jaw bone is lower in males than in females. For children aged 14-15 years and 18-19 years, the hyoid bone horizontal to the cervical spine is more forward in males than in females.
    中国组织工程研究杂志出版内容重点:组织构建;骨细胞;软骨细胞;细胞培养;成纤维细胞;血管内皮细胞;骨质疏松组织工程
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    Digital anatomical analysis of drilling position of the clavicle in coracoclavicular ligament reconstruction
    Chen Yu, Song Xuan, Lu Hua, Zhang Tian-hao, Yao Bing
    2015, 19 (42):  6759-6763.  doi: 10.3969/j.issn.2095-4344.2015.42.007
    Abstract ( 283 )   PDF (896KB) ( 258 )   Save

    BACKGROUND: Coracoclavicular ligament reconstruction with transclavicular-transcoracoid drilling is an effective surgical technique to treat acromioclavicular dislocation. A good drilling in the clavicle leads to a perfect bony tunnel and a good surgery.
    OBJECTIVE: To observe the effects of different drilling positions of the clavicle on the location of bony tunnels in coracoclavicular ligament reconstruction.
    METHODS: Sixty three-dimensional digital models of the clavicle and coracoid process were constructed by Mimics13.0. Virtual transclavicular-transcoracoid bony tunnels were established according to different surgical planes with different drilling positions in the clavicle. Parameters of these bony tunnels were measured, and the safety was evaluated. Option 1: The drilling was made 30 mm distal to the clavicle, located in the center of the front and rear edges of the clavicle surface. Option 2: The drilling was made 40 mm distal to the clavicle, located in the center of the front and rear edges of the clavicle surface. Option 3: The drilling was made at the straight line of tapered nodule tip and the midpoint of the base of the coracoid process, located at the rear edge of the clavicle upper surface.
    RESULTS AND CONCLUSION: Bony tunnels in option 1 were extremely on the inside of the coracoid. Bony tunnels in options 1 and 2 were not in the center of clavicle. Bony tunnels in option 3 were in the center of both 
    clavicle and coracoid. The method of locating the drilling position with a certain distance to the distal clavicle leads to different results in man’s and woman’s models. To ensure that the bony tunnel can pass through the center of clavicle and coracoid, it is suggested to drill at the straight line of tapered nodule tip and the midpoint of the base of the coracoid process and nearby the rear edge of the clavicle upper surface. 

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    Reconstruction of anterior cruciate ligament with allogeneic tendon: a medium-term follow-up
    Jia Yan-bo, Liang Zi-hong, Ren Yi-zhong, Han Chang-xu, Kong Ling-yue, Eerduntu
    2015, 19 (42):  6764-6769.  doi: 10.3969/j.issn.2095-4344.2015.42.008
    Abstract ( 306 )   PDF (2114KB) ( 317 )   Save

    BACKGROUND: Allogeneic tendon has been frequently used to reconstruct the anterior cruciate ligament. However, there is a lack of middle- and long-term clinical evaluation.

    OBJECTIVE: To evaluate the middle- and long-term curative effect of allogeneic tendon reconstruction of the anterior cruciate ligament under arthroscopy.
    METHODS: Twenty-five patients with anterior cruciate ligament injury under allogeneic tendon transplantation were reviewed retrospectively. They were all followed up for 3 years or more. The clinical outcomes were evaluated and compared using Lyshlom score scale, IKDC2000 and Tegner score scale.

    RESULTS AND CONCLUSION: All the patients were followed up. Lysholm scale scores on the knee function, Tegner scores and IKDC scores were significantly increased after transplantation (P < 0.05). No complications of infection and compartment syndrome occurred. Symptoms were relieved and the function of knee joint was improved. There was one case weakly positive for anterior drawer test. Both Lachman test and pivot shift test showed negative signs. Overall, the reconstruction of anterior cruciate ligament with allogeneic tendon has less trauma and complications, with good middle-term efficacy.
    中国组织工程研究杂志出版内容重点:组织构建;骨细胞;软骨细胞;细胞培养;成纤维细胞;血管内皮细胞;骨质疏松组织工程

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    Femoral compression variation in osteoporosis rats after intervention with different drugs
    Qi Bing, Dai Wei-qun, You Jian-yu, Li Xiao-li
    2015, 19 (42):  6770-6775.  doi: 10.3969/j.issn.2095-4344.2015.42.009
    Abstract ( 336 )   PDF (995KB) ( 369 )   Save

    BACKGROUND: The mechanical index is an important method for the evaluation of the therapeutic efficiency of drug treatment for osteoporosis animal models.

    OBJECTIVE: To explore the effects of various drug treatments on osteoporosis through a mechanical performance test about the femoral compression of rats.
    METHODS: Thirty-six Wistar female rats were randomized into six groups: normal control group, model group, Dan Qi particles group, alpha-D3 group, premarin group, ipriflavone group, with six rats in each group. Osteoporosis models were made in all groups except for the normal control group, and after modeling, the rats in different groups were treated with Dan Qi particles, alpha-D3 group, premarin and ipriflavone, respectively. After 15 weeks, the rats were killed by abdominal aortic bloodletting to take out the left and right femurs that were placed on a universal testing machine to perform a compressive test at a speed of 5 mm/min.

    RESULTS AND CONCLUSION: The maximum load, maximum stress, maximum displacement, maximum strain, and elastic modulus were significantly lower in the model group than the other four groups (P < 0.05). There was no difference in different mechanical parameters between alpha-D3 group and model group as well as between Dan Qi particles group and normal control group (P > 0.05). These findings indicate that osteoporosis leads to the variation of compression mechanical properties of the femur. There are good compression mechanical properties of the femur after treatment with premarin and ipriflavone, and Dan Qi particles has the best effect.
    中国组织工程研究杂志出版内容重点:组织构建;骨细胞;软骨细胞;细胞培养;成纤维细胞;血管内皮细胞;骨质疏松组织工程

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    Three-dimensional motion analysis of dominant and nondominant hands under weight-bearing conditions in health elderly people
    Mao Yu-rong, Chen Na, Chen Pei-ming, Chen Song-bin, Li Li-fang, Huang Dong-feng
    2015, 19 (42):  6776-6781.  doi: 10.3969/j.issn.2095-4344.2015.42.010
    Abstract ( 303 )   PDF (862KB) ( 323 )   Save

    BACKGROUND: Differences exist between the action execution of the dominant hand and the nondominant hand during daily lives. With the increasing of the age, the dominant hand and the nondominant hand play an equally important role in the action execution and implementation during daily lives. Previous studies mainly focus on the muscle strength of upper limbs. However, studies on the influence of joint dynamic characteristics and trajectory deviation on the occupational activities have been increased gradually. The three-dimension motion capture and analysis have become the reliable and valid standard of the assessment of the upper limb movement.

    OBJECTIVE:By using the three-dimensional kinematic analyze method, to collect the data of the healthy elderly people using the upper limbs to drink water respectively with the cups of different weights and to investigate whether there is a difference between dominant and nondominant hands under different weight-bearing conditions based on upper limb kinematics
    METHODS: Sixteen right-handed elder people were chosen to be the experimental subjects. The upper limb motion of drinking water with different weight was captured by Vicon Nexus. By Data modeling and trajectory filtering with pipeline and data normalizing with the Matlab, the three-dimensional angle and peak value of the velocity of the should, elbow and wrist joint in the bilateral upper limbs were analyzed and compared when lifting the cups of 100, 200 and 500 g.

    RESULTS AND CONCLUSION: The most significant difference could be found in the three-dimensional movement angle of the elbow joint when holding the weights: there were significant differences in 100 g horizontal plane (X axis) and sagittal plane (Z axis), 200 g frontal plane (Y axis) and sagittal plane (Z axis), and 500 g three-dimensional plane (P < 0.05). Difference could be found in the peak value of three-dimension angular velocity in the shoulder, elbow and wrist joints: 100 g (shoulder joint Px=0.01; elbow joint Py=0.048, Pz=0.007), 200 g (elbow joint Py=0.033, Pz=0.005; wrist joint Py=0.035), 500 g (elbow joint Py=0.027, Pz=0.006) had significant differences (P < 0.05). There was no significant difference in the movement angle and angular velocity when holding different weights with the ipsilateral upper limb (P > 0.05). These results show that there is a difference in the movement angle and angular velocity between the dominant hand and the nondominant hand when drinking water. A great change of movement angle could be found in the X axis of the elbow joint in the dominant hand, and a great change of the movement angle could be found in the Z axis of the elbow joint in the nondominant hand. The angluar velocity in the Y axis has better changes than in the Z axis. The size of the weights has no effect on the movement of bilateral upper limbs.
    中国组织工程研究杂志出版内容重点:组织构建;骨细胞;软骨细胞;细胞培养;成纤维细胞;血管内皮细胞;骨质疏松组织工程

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    Spinal motor neurons from neonatal rats: purification, culture and identification
    Yang Lin, Liu Yang, Lv De-cheng
    2015, 19 (42):  6782-6786.  doi: 10.3969/j.issn.2095-4344.2015.42.011
    Abstract ( 531 )   PDF (914KB) ( 622 )   Save

    BACKGROUND: neurons are post-mitotic cells that are difficult to survive. Isolation, purification and culture of spinal motor neurons are technical difficulties of cell culture technology.
    OBJECTIVE: To establish the culture system of spinal motor neurons from neonatal rats and to identify and determine the purity of spinal cord neurons.
    METHODS: Ventral spinal cord tissues from neonatal rats were made into cell suspension that was subjected to density gradient centrifugation and differential adherence followed by purification culture. Then, the cells on cover plates were identified and classified using immunocytochemical double staining method, and the content of cell components was calculated in combination with fluorescent Hoechst nuclear staining.
    RESULTS AND CONCLUSION: The cells adhered well, and the neurons accounted for 85.8%, among which, motor neurons reached 71.6%, astrocytes accounted for 7.8%, cells negative for neurofilament 200 and glial fibrillary acidic protein were 6.4%. These findings indicate that the ventral spinal cord tissues from neonatal rats combined with density gradient centrifugation and differential adherence can develop high-purity spinal motor neurons.
    中国组织工程研究杂志出版内容重点:组织构建;骨细胞;软骨细胞;细胞培养;成纤维细胞;血管内皮细胞;骨质疏松组织工程

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    Directional and specific characteristics of astrocyte fiber growth and synapse formation
    Cao Jing-li, Li Qi, Wang Ya-jie
    2015, 19 (42):  6787-6792.  doi: 10.3969/j.issn.2095-4344.2015.42.012
    Abstract ( 272 )   PDF (893KB) ( 295 )   Save

    BACKGROUND: Studies have shown that astrocytes participate not only in the passive metabolic and structural support, but also actively in the central nervous system information processing.

    OBJECTIVE: By observing the directionality and specificity of astrocyte fiber growth and synapse formation, to explore the possible mechanisms of astrocytes and neurons in the interaction.
    METHODS: Primary cells from the hippocampi of green fluorescent protein transgenic newborn mice were isolated and cultured, glial fibrillary acidic protein was used as specific marker for astrocytes in immunofluorescence staining, and then the results were observed and scanned by confocal microscope.
    RESULTS AND CONCLUSION: The elongation of astrocyte fibers was directional and specific, and terminated to the pyramidal cells nearby. The end of astrocyte fibers and pyramidal cells contributed to the synapse  formation. However, not all the projections were toward the adjacent cells, and synapse-like structure was not only formed nearby. The results implicated that both astrocytes fiber elongation and synapse formation have the directionality and specificity, providing evidence for further studies on signal transduction between astrocytes and pyramidal cells.
    中国组织工程研究杂志出版内容重点:组织构建;骨细胞;软骨细胞;细胞培养;成纤维细胞;血管内皮细胞;骨质疏松组织工程
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    Human cartilage glycoprotein-39 induces chondrogenesis of precartilaginous stem cells
    Chen Yue-ping, Dong Pan-feng, Yuan Zhen-zhong, Rao Yi, Li Jin-huan, Kang Jie, Zhang Xiao-yun
    2015, 19 (42):  6793-6797.  doi: 10.3969/j.issn.2095-4344.2015.42.013
    Abstract ( 424 )   PDF (1898KB) ( 376 )   Save

    BACKGROUND: Studies have shown that human cartilage glycoprotein-39 has a certain relationship to articular cartilage degeneration and repair, but the mechanism of action is not very clear.
    OBJECTIVE: To investigate the effect of human cartilage glycoprotein-39 on chondrogenesis of precartilaginous stem cells.
    METHODS: Precartilaginous stem cells were isolated from the adult articular cartilage. Cells which could express CD105 and CD166 were detected using flow cytometry followed by isolation and purification. Isolated precartilaginous stem cells werecultured using monolayer method, and then, passage 2 cells were cultured in the medium containing human cartilage glycoprotein-39 and normal chondrogenic medium for 14 days, respectively. Immunohistochemical staining was used to observe expression of type II collagen and gross observation was 
    done for evaluation of cartilage formation.
    RESULTS AND CONCLUSION: The precartilaginous stem cells isolated from the adult articular cartilage could express CD105 and CD166. After induction, differentiated precartilaginous stem cells gradually gathered and formed nudes. The induced cells were positive for type II collagen; after induction by human cartilage glycoprotein-39, the nodules became larger and the expression of type II collagen was increased. These findings indicate that precartilaginous stem cells with chondrogenic ability can be isolated from the adult articular cartilage, and can be induced to differentiate into chondrocytes, in which human cartilage glycoprotein-39 plays an important role. 
    中国组织工程研究杂志出版内容重点:组织构建;骨细胞;软骨细胞;细胞培养;成纤维细胞;血管内皮细胞;骨质疏松组织工程

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    Significance of erythropoietin for the embryonic development of the lens
    Guo Qing-min, Meng Xu-xia, Tian Ye, Hu Die
    2015, 19 (42):  6798-6802.  doi: 10.3969/j.issn.2095-4344.2015.42.014
    Abstract ( 233 )   PDF (4824KB) ( 279 )   Save

    BACKGROUND: Studies have found that erythropoietin has a protective effect on embryonic development of the retina, but there are rare studies concerning the embryonic development of the lens.

    OBJECTIVE: To observe the difference in erythropoietin expression in the lens from Wistar rats at different embryonic periods and to investigate the effect and significance of erythropoietin in the embryonic development of the lens.
    METHODS: Clean Wistar rats with pregnancy for 10 days (n=5), 12 days (n=5), 14 days (n=5), 16 days (n=5), 18 days (n=5), 20 days (n=5) were randomly collected and divided into six groups. Every two embryonic ratsof the different 30 pregnant rats were obtained randomly by the caesarean operation under ketamine-induced anesthesia. The eye tissues of all the embryonic rats were isolated and cut into sections. The expression of erythropoietin protein and mRNA in rat lens was detected by immunohistochemistry and reverse t ranscription-PCR, respectively.

    RESULTS AND CONCLUSION: Erythropoietin was distinctly expressed at the six different embryo stages, and the expression of erythropoietin protein and mRNA gradually increased from embryonic day 10 to embryonic day 16, and decreased from embryonic day 10 to embryonic day 20. There were significant differences between the six groups. These findings indicate that the expression of embryonic appears in a low to high to low fashion during the embryonic development of Wistar rats, which may be closely associated with the developing procedure of lens. 
    中国组织工程研究杂志出版内容重点:组织构建;骨细胞;软骨细胞;细胞培养;成纤维细胞;血管内皮细胞;骨质疏松组织工程

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    Effect of static stretching and strength exercise of the lower limb on the balance function of the elderly
    Mao Zhi-bang, Zhang Ling-li, Lai Xiao-yong, Yu Xiao-ming, Wang Hong-lin, Jiang Li-ming,Yu Zhu-sheng
    2015, 19 (42):  6803-6807.  doi: 10.3969/j.issn.2095-4344.2015.42.015
    Abstract ( 433 )   PDF (869KB) ( 332 )   Save

    BACKGROUND: After entering the aging society, improving the balance function to prevent falls and reduce incidence of fractures in the elderly has received widespread attentions in all aspects.

    OBJECTIVE: To design a training program for the elderly, involving static stretching and strength exercise of lower limb, in order to study the combined effect on the balance function of the elderly.
    METHODS: Static stretching and strength training of the lower limbs were carried out in 28 elderly persons who met the inclusion criteria for 3 months, three times a week, once for 60 minutes. Before and after exercise training, the static balance ability with foot standing, eyes open and eyes closed was evaluated using the PRO-KIN balance instrument; the dynamic balance ability and lower limb strength were evaluated using treadmill test with eyes closed and chair-stand test. Evaluation results before and after exercise were compared and anayzed.

    RESULTS AND CONCLUSION: In the eyes open state, the average X-axis coordinate and exercise-induced ellipse area at the center of pressure were significant different before and after exercise (P < 0.01); the mean velocities at the left and right direction were of significant difference before and after exercise (P < 0.05); the results of treadmill test with eyes closed and chair-stand test also presented very significant differences before and after exercise (P < 0.01). Static stretching combined with lower limb strength exercise can effectively improve the balance function and lower limb strength of the elderly, which can be used as an effective exercise way to prevent the falls in the elderly.
    中国组织工程研究杂志出版内容重点:组织构建;骨细胞;软骨细胞;细胞培养;成纤维细胞;血管内皮细胞;骨质疏松组织工程

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    Body composition and resting energy expenditure in perimenopausal women
    Liu Yu-lan, Ruan Xiang-yan, Wu Hong-qin
    2015, 19 (42):  6808-6812.  doi: 10.3969/j.issn.2095-4344.2015.42.016
    Abstract ( 346 )   PDF (796KB) ( 296 )   Save

    BACKGROUND: Aging, lack of estrogen and few activities may lead to the decrease of resting energy expenditure in menopause women so as to induce the body composition changes, which can result in disease, such as obesity and fracture.

    OBJECTIVE: To explore the change of body composition and resting metabolic rate among premenopausal, perimenopausal and postmenopausal women.
    METHODS: 2 312 women who came to outpatient clinic were recruited, including 1 310 premenopausal women, 790 perimenopausal women and 212 postmenopausal women. Body composition and resting energy expenditure were measured in each woman.
    RESULTS AND CONCLUSION: (1) Body fat percentage of perimenopausal and postmenopausal women was significantly higher than that of pre-menopausal women. (2) There was no significant difference in resting metabolic rate among the three groups. When adjusted body weight, the resting energy expenditure per kilogram was significantly lower in perimenopausal women than in premenopausal women. (3) Bone mineral density, femoral neck strength, lower limb maximal strength, and leg muscle distribution coefficient were significantly lower in postmenopausal women than in perimenopausal and premenopausal women (P < 0.05). (4) Weight and leg muscle distribution coefficient were positively correlated with the resting energy expenditure per kilogram, and height, body mass index, body fat percentage were negatively correlated with the resting energy expenditure per kilogram. These findings indicate that the body composition can change in women after menopause, such as body fat increasing, muscle content decreasing, and bone mass loss. There is a certain relationship between the change in body composition and resting energy expenditure.
    中国组织工程研究杂志出版内容重点:组织构建;骨细胞;软骨细胞;细胞培养;成纤维细胞;血管内皮细胞;骨质疏松组织工程
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    Neuroprotective effects of salvianolic acid B against Schwann cell injury induced by lipopolysaccharide
    Bi Lian-yong
    2015, 19 (42):  6813-6818.  doi: 10.3969/j.issn.2095-4344.2015.42.017
    Abstract ( 342 )   PDF (1065KB) ( 565 )   Save

    BACKGROUND: Salvianolic acid B is an effective monomer component of Salvia miltiorrhiza, which has been shown in recent years to have neuroprotective role and to promote nerve recovery, but its mechanism is not clear.

    OBJECTIVE: To explore the protective mechanism of salvianolic acid B on Schwann cells in rats with spinal cord injury.
    METHODS: Schwann cells of Sprague-Dawley rats were cultured and divided into normal control group, model group, 10 μmol/L methylprednisolone group and salvianolic acid B group (0.1, 1, 10, 100 μmol/L). Models of Schwann cell injury induced by lipopolysaccharide were established in all the groups except the normal control group. After intervention, growth curve and proliferative activity of Schwann cells were detected, and protein and gene expressions of β-catenin and nuclear factor-κB were observed.
    RESULTS AND CONCLUSION: At 48, 72, 96 hours after intervention, the cell viability of the model group was significantly lower than that of the normal control group (P < 0.01). Compared with the model group, the cell viability of salvianolic acid B group (10 μmol/L) was significantly increased at 72 and 96 hours (P < 0.05); the expressions of nuclear factor-κB protein and mRNA in the methylprednisolone group and salvianolic acid B group were declined significantly (P < 0.01), but the expressions of β-catenin mRNA and protein in the salvianolic acid B group significantly increased (P < 0.05). These results suggest that salvianolic acid B improves the viability of Schwann cells which are stimulated with lipopolysaccharide, suppresses expression of nuclear factor-κB mRNA and protein, and promotes the expression of β-catenin mRNA and protein. Above may be one of the mechanisms which salvianolic acid B protects Schwann cells.
    中国组织工程研究杂志出版内容重点:组织构建;骨细胞;软骨细胞;细胞培养;成纤维细胞;血管内皮细胞;骨质疏松组织工程
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    Maxillofacial and periodontal tissue-engineered bone: vascularization and osteogenesis strategies
    Zhang Xin-yue, Li Yan, Li Chen-jun
    2015, 19 (42):  6819-6825.  doi: 10.3969/j.issn.2095-4344.2015.42.018
    Abstract ( 342 )   PDF (1062KB) ( 414 )   Save

    BACKGROUND: Maxillofacial bone and periodontal tissue defect is one of the important diseases that affect human functionality and aesthetic appearance, and bone tissue engineering becomes the main means to repair maxillofacial and periodontal tissue defects. Currently, the basic mode is constructed by the combination of co-culture of seed cells and cells, scaffolds and micro-environment. Pre-vascularization and rapid osteogenesis of tissue-engineered bone can reduce implant necrosis and absorption, and improve repair success rate.
    OBJECTIVE: To summarize the new progress of bone tissue engineering used in the oral and maxillofacial and periodontal tissue in the past 5 years.
    METHODS: CNKI database and PubMed database from 2010 to 2015 were searched using the keywords of “oral and maxillofacial, bone tissue engineering, bone regeneration, vascularization, genetic modification, seed cells, support material, microenvironment” in Chinese and English, respectively. After elimination of independent and repetitive studies, 68 articles were included in result analysis.
    RESULTS AND CONCLUSION: The tissue-engineered bone has achieved tremendous progress in the repair of oral and maxillofacial and periodontal tissue defects. The three-dimensional scaffold with gene-modified seed cells can effectively promote the vascularization, improve the osteogenic effect and increased the probability of success in mandibular defect repair. In addition, tissue-engineered bone implantation into the alveolar ridge defects or fresh extraction fossa can effectively restore and preserve alveolar ridge height and width, to ensure a good bone condition for subsequent restorative treatment. After the implantation of tissue-engineered bone, different external environmental stimuli could be loaded at defect sites, and the extracellular matrix components or signal pathway could be adjusted to change the process of vascularization. Vascularization is a premise condition for the establishment of an effective blood circulation to ensure the success of scaffold implantation.
    中国组织工程研究杂志出版内容重点:组织构建;骨细胞;软骨细胞;细胞培养;成纤维细胞;血管内皮细胞;骨质疏松组织工程

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    Role of insulin-like growth factor in the orthodontic periodontal tissue remodeling
    Lang Yao-ling, Guan Xiao-yan, Liu Jian-guo
    2015, 19 (42):  6826-6831.  doi: 10.3969/j.issn.2095-4344.2015.42.019
    Abstract ( 231 )   PDF (1077KB) ( 279 )   Save

    BACKGROUND: Orthodontic treatment is a mechanical force for tooth to cause the remodeling of periodontal tissue, produced by the tooth movement. The main aspect of orthodontic periodontal tissue remodeling is the alveolar bone. Insulin-like growth factor is an important factor in the remodeling of periodontal tissue, which plays an important role in the growth, differentiation and growth of the cells.
    OBJECTIVE: To review the role of insulin-like growth factors in periodontal tissue remodeling. 
    METHODS: A computer-based retrieval of PubMed, CNKI and Guizhou Province Digital Library Database was
    performed to search articles related to the role of insulin-like growth factors in periodontal tissue remodeling. The keywords were “insulin-like growth factor; periodontal tissue; remodeling” in English and Chinese, respectively.
    RESULTS AND CONCLUSION: Insulin like growth factor belongs to the insulin family, a kind of peptides, which can promote the migration, proliferation, differentiation, collagen and matrix synthesis and alkaline phosphatase activity of osteoblasts, fibroblasts and mesenchymal cells in the periodontal ligament. It also plays an important role in the repair of injury. During orthodontic treatment, the use of suitable orthodontic force combined with insulin-like growth factor can promote periodontal tissue remodeling, accelerate the orthodontic tooth movement and shorten the treatment time for patients.
     

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    How to build a tissue-engineered intervertebral disc annulus fibrosus that is more close to the natural one?
    Yuan De-chao, Chen Zhu, Feng Da-xiong, Feng Gang
    2015, 19 (42):  6832-6837.  doi: 10.3969/j.issn.2095-4344.2015.42.020
    Abstract ( 293 )   PDF (910KB) ( 407 )   Save

    BACKGROUND: Tissue-engineered intervertebral disc has provided a new biological therapeutic approach for intervertebral disc degeneration. Tissue-engineered annulus fibrosus is one key step of constructing a complete tissue-engineered intervertebral disc.
    OBJECTIVE: To sum up the research progress of tissue-engineered annulus fibrosus from the following aspects: structural features, scaffold materials, seed cells.
    METHODS: PubMed database and Wanfang database (2000-2015) were retrieved by the key words of “tissue engineering, intervertebral disc, annulus fibrosus, seed cell, scaffold, construction” in Chinese and in English, respectively. According to inclusion and exclusion criteria, 48 literatures were involved for summarization. 
    RESULTS AND CONCLUSION: Previous studies about tissue-engineered annulus fibrosus only focused on cell adhesion, proliferation and extracellular matrix secretion on the scaffold. Currently, tissue-engineered annulus fibrosus exhibit similar features to the natural annulus fibrosus in the following aspects: cell function, tissue structure and mechanical features, and relevant animal experiments have achieved certain results in animal experiments. However, it is still difficult to build a tissue-engineered annulus fibrosus entirely similar to the natural one, and we need to further improve scaffold materials, culture conditions, collection of seed cells. The current strategies of annulus fibrosus construction still focus on single phase of scaffold, and the biphasic scaffold and complete intervertebral disc scaffold will be the trend of the researches. Technology of induced differentiation of stem cells provides a broach source of seed cells for tissue-engineered annulus fibrosus.
    中国组织工程研究杂志出版内容重点:组织构建;骨细胞;软骨细胞;细胞培养;成纤维细胞;血管内皮细胞;骨质疏松组织工程

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    Bone metabolism and gut microbiota
    Yuan Si-yuan, He Fang, Sheng Tong, Ishimi Yoshiko, Wang Xin-xiang
    2015, 19 (42):  6838-6842.  doi: 10.3969/j.issn.2095-4344.2015.42.021
    Abstract ( 471 )   PDF (820KB) ( 623 )   Save

    BACKGROUND: The gut microbiota in our intestine performs numerous useful functions and has a major impact on the host’s health. Recently some studies have revealed that the gut microbiota cannot only control intestinal activity but also affect bone metabolism by regulating the immune system.

    OBJECTIVE: To review the new research development in the effects of gut microbiota on bone metabolism.
    METHODS: We retrieved the PubMed database using “gut microbiota, immune system, bone metabolism, osteoporosis” as keywords. A total of 46 articles were included which were related to gut microbiota, immune system and bone metabolism. For the articles in the same field, those published recently or in authorized journals were selected.

    RESULTS AND CONCLUSION: Gut microbiota-osteoporosis research will bridge the gaps between bone physiology, gastroenterology, immunology, and microbiology. In vivo experiments in the germ-free mice and human body have found that the gut microbiota has important effects on bone metabolism, and the intervention of antibiotics, probiotics and prebiotics has further confirmed the effects of gut microbiota on bone mass. The gut microbiota has more obvious effects on bone mass in the adolescent and post-menopause periods.
    中国组织工程研究杂志出版内容重点:组织构建;骨细胞;软骨细胞;细胞培养;成纤维细胞;血管内皮细胞;骨质疏松组织工程

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    SenseWear Pro Armband: a monitoring device of physical activity energy consumption
    Wang Si-ya, Zhuang Jie, Zhu Zheng
    2015, 19 (42):  6843-6848.  doi: 10.3969/j.issn.2095-4344.2015.42.022
    Abstract ( 664 )   PDF (1018KB) ( 721 )   Save

    BACKGROUND: SenseWear Pro Armband (SWA) is a monitoring device of physical activity energy consumption which consists of many kinds of motion sensors, and it has been widely used in the study of physical activity energy consumption in recent years.

    OBJECTIVE: To summarize the SWA accuracy, software version, time of wear as well as its application in different populations and at different physical activity levels, for the purpose of providing some practical reference in the future usage of SWA.
    METHODS: A computer-based search of CNKI (full-text database), PubMed and Web of Science databases was done for relevant articles published before January 2015 using the keywords of “physical activity, sensewear pro armband, energy expenditure, review” in Chinese and English, respectively. Articles published in core journals or SCI database were preferred.
    RESULTS AND CONCLUSION: Double labeled water method and indirect measurement method serve as standard criteria, and the relevant study shows that SWA has higher accuracy and can effectively evaluate the daily physical activity energy consumption. But under the special circumstances, there are some differences, such as different people and different exercise intensities. Meanwhile, to determine the appropriate wearing time and software version for data statistics are also one of the factors influencing the accuracy of the final result. SWA can accurately predict physical activity energy consumption, but for different populations and exercise intensities, it is necessary to improve the further calculation formula of SWA.
    中国组织工程研究杂志出版内容重点:组织构建;骨细胞;软骨细胞;细胞培养;成纤维细胞;血管内皮细胞;骨质疏松组织工程
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    Re-cognition of exercise-induced central and peripheral fatigue
    Tian Feng
    2015, 19 (42):  6849-6854.  doi: 10.3969/j.issn.2095-4344.2015.42.023
    Abstract ( 462 )   PDF (856KB) ( 526 )   Save

    BACKGROUND: Animal exhaustion models are used mostly in exercise-induced fatigue research, in which complete exhaustion is confused with sports fatigue.

    OBJECTIVE: By understanding of exercise-induced fatigue, understanding the mechanism of its occurrence, to grasp the reasonable and effective control measures, which to eliminate fatigue is very important for improving public health, improving sports performance and preventing sports injuries.
    METHODS: A computer-based search of Medline, Embase, CBMdisc and CNKI was used for articles related to exercise-induced fatigue published from 1966 to 2014. The keywords were “exercise-induced fatigue, peripheral fatigue; central fatigue, exercise-induced fatigue” in English and Chinese, respectively. Finally, 49 articles were included in result analysis.

    RESULTS AND CONCLUSION: The criteria and condition control of exercise-induced fatigue should be recognized. The mechanisms of peripheral fatigue and central fatigue are still unclear. The peripheral fatigue may locate in the neuromuscular junction, T system, sarcoplasmic reticulum, mitochondria and myocardial cell membrane with the corresponding material changes. During the exercise-induced central fatigue, there are corresponding changes in the neurotransmitters produced by the central nervous system, transfers quality and neural hormone.
    中国组织工程研究杂志出版内容重点:组织构建;骨细胞;软骨细胞;细胞培养;成纤维细胞;血管内皮细胞;骨质疏松组织工程

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    Clinical application and progression of warm acup-moxibustion as an analgesic therapy
    Zheng Han-dan, Zhao Ji-meng Wu Lu-yi, Huang Ren-jia, Zhu Yi, Wang Shuo-shuo, Hu Zhi-hai, Liu Hui-rong, Dou Chuan-zi, Huang Yan, Wu Huan-gan
    2015, 19 (42):  6855-6860.  doi: 10.3969/j.issn.2095-4344.2015.42.024
    Abstract ( 430 )   PDF (931KB) ( 264 )   Save

    BACKGROUND: Warm acup-moxibustion is an effective treatment in Chinese traditional medicine, which combines acupuncture with moxibustion. With an immediate analgesia or/and long-term effect, warm acup-moxibustion has been used clinically for acute and chronic pain.

    OBJECTIVE: To analyze the progress in the studies about clinical application of warm acup-moxibustion as analgesic therapy for acute and chronic pain and to discuss the influential factors.
    METHODS: CNKI, VIP and Wanfang databases were searched for relevant articles published between January 1995 and July 2015 using the keywords of “warm needling; warm acup-moxibustion; pain; analgesic” in Chinese. According to the exclusion and inclusion criteria, 51 articles were included in result analysis.
    RESULTS AND CONCLUSION: Compared with ordinary acupuncture, medicine and other treatment methods, warm acup-moxibustion can play an analgesic role in multiple systems, which is better than ordinary acupuncture and Western medicine. Warm acup-moxibustion combined with drugs and acupuncture techniques can increase the analgesic effect and improve joint function activity. Warm acup-moxibustion with the overall regulatory role plays an effective analgesic pain role in the multiple systems and shortens the duration of treatment, which is an effective method for treating pain and worthy of further promotion and application.
    中国组织工程研究杂志出版内容重点:组织构建;骨细胞;软骨细胞;细胞培养;成纤维细胞;血管内皮细胞;骨质疏松组织工程
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    Knowledge base, research front and hot spot analysis of Cu-Zn superoxide dismutase
    Zou Yu, Li Yan-jun Yang Shuang, Zhang Qing-wen
    2015, 19 (42):  6861-6867.  doi: 10.3969/j.issn.2095-4344.2015.42.025
    Abstract ( 279 )   PDF (1430KB) ( 311 )   Save

    BACKGROUND: Studies have found that Cu-Zn superoxide dismutase (SOD1) is associated with familial amyotrophic lateral sclerosis, opening up a new way of studying molecular genetics. So far more than 100 kinds of SOD1 gene mutations have been found.

    OBJECTIVE: To analyze the hot spot, research front and knowledge base of amyotrophic lateral sclerosis visually.
    METHODS: Totally 4 693 relevant articles published from 2005 to 2014 were retrieved from Web of Science in ISI with “Cu-Zn superoxide dismutase” or “SOD1” as search keywords. With the aid of CiteSpace III software, the visualization mapping of the network in co-cited articles and keywords was drawn to reveal knowledge base, hot spots and research front of SOD1. The parameters include the number of published papers and citations within 10 years, distribution of research countries and institutions, main source journals, research area of highly cited papers, keywords with high-frequency and emerging keywords with high-frequency in recent 5 years.

    RESULTS AND CONCLUSION: The number of published papers and citations in a year showed a trend of sustained growth. United State, China and Japan rank the top three in this area, in which Chinese Academy of Sciences has a great influence among the research institutions. The research fields of SOD1 focus on neurosciences and neurology, biochemistry and molecular biology and so on. The high impact factors of journals with a large number of articles reflect the importance and innovation of this research. Ten high-cited articles consist of the knowledge base on SOD1, directing to the finding of different sites of SOD1 mutation and the measurement of protein concentrations and activity of SOD. The hot spots of SOD1 mainly focus on oxidative stress, familial amyotrophic lateral sclerosis caused by SOD1 mutation and different types of transgenic animal models. The research fronts mainly focus on the finding of pathogenesis in amyotrophic lateral sclerosis, such as the aggregation of TDP-43, the interaction between astrocytes and motor neurons, optineurin and the inhibitor of nuclear factor-κB, hexanucleotide repeat expansion in C9ORF72 and autophagy.
    中国组织工程研究杂志出版内容重点:组织构建;骨细胞;软骨细胞;细胞培养;成纤维细胞;血管内皮细胞;骨质疏松组织工程

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    Methylprednisolone for acute spinal cord injury: a Meta-analysis of therapeutic efficacy and adverse reactions
    Zhao Wen-tao, Yuan Hong-bing, Li Pan-pan, Zhang Hai-feng, Liu Ming-qing
    2015, 19 (42):  6868-6874.  doi: 10.3969/j.issn.2095-4344.2015.42.026
    Abstract ( 319 )   PDF (1163KB) ( 333 )   Save

    BACKGROUND: Methylprednisolone has been used for the treatment of acute spinal cord injury but there is a dispute about the efficacy and safety of methylprednisolon.

    OBJECTIVE: To evaluate the efficacy and safety of methylprednisolon based on system review.
    METHODS: PubMed database, EMBASE database, Cochrane Library, ISI Web of knowledge, CBM database, VIP database, CNKI database and Wanfang database were searched from their start year up to December 2014 for relevant randomized clinical trials on the treatment of acute spinal cord injury with methylprednisolon.
    RESULTS AND CONCLUSION: Twelve randomized clinical trials with 642 acute spinal cord injury patients were included. The results of the Meta analysis showed: methylprednisolone+conventional therapy was better to improve American Spinal Injury Association (ASIA) motor function score, ASIA touch sensation score, ASIA pinprick sensation score and the overall Frankel score than the conventional therapy alone (control group) with statistical significance (P < 0.05). In the aspect of safety, the methylprednisolone group had higher death ratio, digestive tract reaction ratio and urinary infection ratio than the control group but with no statistical significance. The gastrointestinal bleeding ratio and lung infection ratio was significantly higher in the methylprednisolone group than the control group (P < 0.05). We conclude that methylprednisolone has protective effect on acute spinal cord injury, but the main side effects are gastrointestinal bleeding and lung infection. There is a need for high-quality randomized controlled trials to prove the efficiency and safety of methylprednisolone.
    中国组织工程研究杂志出版内容重点:组织构建;骨细胞;软骨细胞;细胞培养;成纤维细胞;血管内皮细胞;骨质疏松组织工程
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    High-dose methylprednisolone sodium succinate therapy for acute spinal cord injury in adults: a Meta-analysis
    Liu Xiang-dong, Wang Lei, Dong Zhe, Ma Yan-hui, Hu Fang, Ma Gai-ping, Wang Feng-feng,Yang Sheng-jun
    2015, 19 (42):  6875-6881.  doi: 10.3969/j.issn.2095-4344.2015.42.027
    Abstract ( 253 )   PDF (1180KB) ( 327 )   Save

    BACKGROUND: Some control studies attempt to answer the advantages and disadvantages of high-dose methylprednisolone sodium succinate therapy for acute spinal cord injury in adults, but have arrived at different conclusions.

    OBJECTIVE: To explore the therapeutic efficacy of high-dose methylprednisolone sodium succinate therapy on acute spinal cord injury in adults by Meta analysis.
    METHODS:PubMed, Embase, Cochranel Library, CBMdisc, VIP and WanFang Databases were searched by computer, and relevant Chinese and English orthopedic journals were retrieved by hand. Controlled trials related to high-dose methylprednisolone sodium succinate therapy of acute spinal cord injury in adults were included. The methodology quality of included trials was critically assessed. RevMan 5.0 software was used for data analysis.
    RESULTS AND CONCLUSION: Nine clinical controlled trials were included. Meta-analysis results showed that compared with the conventional therapy, the neurological recovery rate after 24 hours of administration,  pneumonia incidence and gastrointestinal reactions increased significantly after high-dose methylprednisolone sodium succinate therapy. However, there were no statistical differences in the rate of urinary tract infection, nonunion rate and stress ulcer incidence between these two therapies. These findings indicate that the high-dose methylprednisolone sodium succinate therapy on acute spinal cord injury in adults has better outcomes in neurological function recovery, but can lead to higher incidence of lung infection and gastrointestinal reactions. Therefore, lung infection and gastrointestinal reactions should be avoided as much as possible during the course of treatment.
    中国组织工程研究杂志出版内容重点:组织构建;骨细胞;软骨细胞;细胞培养;成纤维细胞;血管内皮细胞;骨质疏松组织工程
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    Effects of hypothermic machine perfusion versus static cold storage of kidney allografts on transplant outcomes: a Meta-analysis
    Hu Xiao-yan, Wang Yan-feng, Ye Qi-fa, Chen Zhi-quan, Fan Xiao-li, Guo Yi, Li Ning
    2015, 19 (42):  6882-6888.  doi: 10.3969/j.issn.2095-4344.2015.42.028
    Abstract ( 336 )   PDF (1149KB) ( 326 )   Save

    BACKGROUND: At present, there are a number of articles about hypothermic machine perfusion versus static cold storage of kidney allografts; however, the conclusions are various. Furthermore, due to the limitation of single sample size, there is a lack of objective evaluation on the merits and demerits of hypothermic machine perfusion.

    OBJECTIVE: To compare the prognostic outcomes of hypothermic machine perfusion and static cold storage of kidney allografts.
    METHODS: A computer-based search of PubMed, sinoMed, EMbase, Web of Science, the Cochrane Central Register of Controlled Trials (CENTRAL), Wanfang and CNKI databases were searched from their establishment to March 4, 2015 to screen the randomized controlled trials (RCTs) about hypothermic machine perfusion versus static cold storage for kidney transplantation. Meanwhile, the references of included RCTs were also searched by hand. After study selection, RCTs screening, data extraction and quality assessment were conducted by two reviewers independently. Meta-analyses were performed by using the RevMan5.3.0 software. The quality of evidence was assessed by using the GRADEpro3.6 software.

    RESULTS AND CONCLUSION: Six articles were included, involving 619 cases undergoing hypothermic machine perfusion and 620 cases undergoing static cold storage. The results of Meta-analyses showed that the incidence of primary graft non-function, incidence of delayed graft function of functional kidney allografts, and incidence of delayed graft function were significantly lower in the hypothermic machine perfusion group than the static cold storage group (P < 0.05 or 0.01). There were no differences in the 1-year recipient survival rate and 1-year allograft survival rate between the two groups (P > 0.05). These findings indicate that the hypothermic machine perfusion only can reduce the incidence of postoperative complications to maintain the function of kidney, but cannot improve the 1-year recipient survival rate and 1-year allograft survival rate. Hypothermic machine perfusion has no advantage on the long-term preservation of donor organ compared with the static cold storage. 
    中国组织工程研究杂志出版内容重点:组织构建;骨细胞;软骨细胞;细胞培养;成纤维细胞;血管内皮细胞;骨质疏松组织工程

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