Effects of eucommia alcohol extract on a rabbit model of mandibular distraction osteogenesis

doi:10.3969/j.issn.2095-4344.2015.42.001

Abstract

Abstract:

BACKGROUND: Long course of treatment and complications limit the extensive application of distraction osteogenesis, which cannot meet the clinical requirements.
OBJECTIVE: To investigate the effect of general administration of eucommia alcohol extract on new bone regeneration based on the rabbit model of mandibular distraction osteogenesis.
METHODS: Twenty-four New Zealand white rabbits were randomly divided into experimental group and control group. Unilateral mandibular distraction osteogenesis model was established by 1 mm/12 h distraction protocol. During the distraction period, the rabbits in the experimental group were intragastrically administered with eucommia alcohol extract and the control animals received the same amount of physiological saline, respectively. Six weeks later, the animals were sacrificed for osteogenic testing.
RESULTS AND CONCLUSION: New bone formation was observed in the distracted gap in both groups. However, the amount, mineralization and biomechanical strength of new regenerated bone in the experimental group were obviously greater than those in the control group by histological observation, dual energy X-ray
absorptiometry, micro-CT and biomechanical test. General administration of eucommia alcohol extract can markedly promote distraction osteogensis in rabbit mandibular osteodistraction.

中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程

Key words: Rabbits, Mandible, Eucommiaceae, Tissue Engineering

Jiang Xiao-wen, Zhang Yi, Fan Xiao-sheng, Deng Xuan. Effects of eucommia alcohol extract on a rabbit model of mandibular distraction osteogenesis[J]. Chinese Journal of Tissue Engineering Research, 2015, 19(42): 6725-6729.

Figures/Tables 6

References

[1] 胡静.颌骨牵张成骨的临床及基础研究[J].中华口腔医学杂志, 2005, 40(1):10-12.

[2] Earley M, Butts SC. Update on mandibular distraction osteogenesis. Curr Opin Otolaryngol Head Neck Surg.2014; 22(4):276-283.

[3] Verlinden CR,van de Vijfeijken SE,Tuinzing DB,et al. Complications of mandibular distraction osteogenesis for acquired deformities: a systematic review of the literature.Int J Oral Maxillofac Surg.2015;44(8):956-64.

[4] Ando Y, Matsubara K, Ishikawa J, et al. Stem cell-conditioned medium accelerates distraction osteogenesis through multiple regenerative mechanisms.Bone.2014; 61(4):82-90.

[5] Jiang XW, Zou SJ, Ye B, et al.bFGF-Modified BMMSCs enhance bone regeneration following distraction osteogenesis in rabbits. Bone.2010;46(4):1156-1161.

[6] Jiang X, Chen Y, Fan X, et al. Osteogenesis and mineralization in a rabbit mandibular distraction osteogenesis model is promoted by the human LMP-1 gene. J Orthop Res.2015;33(4):521-526.

[7] Zheng LW,Wong MC,Rabie AB,et al.Evaluation of recombinant human bonemorphogeneticprotein-2 in mandibular distraction osteogenesis in rabbits: Effect of dosage and number of doses on formation of bone. Br J Oral Maxillofac Surg.2006;44(6):487-494.

[8] Jiang X,Chen Y,Lu K,et al.GYY4137 promotes bone formation in a rabbit distraction osteogenesis model: a preliminary report.J Oral Maxillofac Surg.2015;73(4):732.e1-e6.

[9] Salem KH, Schmelz A. Low-intensity pulsed ultrasound shortens the treatment time in tibial distraction osteogenesis. Int Orthop.2014;38(7):1477-82.

[10] Abd-Elaal AZ, El-Mekawii HA, Saafan AM, et al. Evaluation of the effect of low-level diode laser therapy applied during the bone consolidation period following mandibular distraction osteogenesis in the human. Int J Oral Maxillofac Surg. 2015; 44(8):989-997.

[11] 郭延伟,李松,房殿吉.杜仲水/醇提取物促进兔脂肪基质干细胞增殖及成骨分化[J].中国组织工程研究,2012,16(32):5959-5962.

[12] 刘丽君.杜仲化学活性成分及其药理学研究慨况[J].亚太传统医药,2013,9(5):82-83.

[13] 戚向阳,陈维军,张声华,等.杜仲粉的药理作用及其有效成分特性研究[J],中国食品学报,2002.2(4):42-47.

[14] He X,Wang J,Li M, et al.Eucommia ulmoides Oliv.: ethnopharmacology, phytochemistry and pharmacology of an important traditional Chinese medicine. J Ethnopharmacol. 2014;151(1):78-92.

[15] 年华,徐玲玲,马明华,等.抗骨质疏松中药的研究现状[J].上海中医药大学学报，2008,22(4):90-93.

[16] 肖润梅，陈勇.药材醇提取物对骨质疏松小鼠生化指标影响的比较[J].上海师范大学学报,2007,36(3):85-88.

[17] 张贤,朱丽华,钱晓伟,等.杜仲醇提取物诱导骨髓间充质干细胞成骨分化中的Wnt信号途径[J].中国组织工程研究,2012,16(45): 8520-8523.

[18] Zhang R, Pan YL, Hu SJ,et al.Effects of total lignans from Eucommia ulmoides barks prevent bone loss in vivo and in vitro. J Ethnopharmacol. 2014;155(1):104-112.

[19] Zhang R, Liu ZG, Li C, et al. Du-Zhong (Eucommia ulmoides Oliv.) cortex extract prevent OVX-induced osteoporosis in rats. Bone. 2009;45(3):553-559.

崔永峰,张永斌,李刚,等.杜仲对兔骨折愈合影响的光镜观察实验研究[J].中国比较医学杂志,2005,15(3):154-156.