Repairing skin defects in nude mice using tissue-engineered skin loading keratinocyte growth factor nanocapsules

doi:10.3969/j.issn.2095-4344.2015.42.005

Abstract

Abstract:

BACKGROUND: As an emerging technology, tissue-engineered skin has great application prospects. Keratinocyte growth factor (KGF) is proved to promote the proliferation of epidermal cells.
OBJECTIVE: To evaluate the effect and characteristics of tissue-engineered skin carrying KGF nanocapsules in repairing skin defects of nude mice.
METHODS: (1) The acellular dermal matrix loading KGF (KGF-ADM) was constructed. The human epidermal stem cell population and fibroblasts were captured and cultivated, and then identified. Epidermal stem cells were cultivated on the KGF-ADM and their growth was observed. The tissue-engineered skin loading KGF nanocapsules was transplanted onto the full-skin defects on the back of nude mice compared with a blank group without keratinocyte growth factor nanocapsules and a control group with skin autograft. In 2, 4 and 6 weeks after transplantation, the contraction and histological healing of the skin were observed respectively. Then anti-human keratin 10-FITC and β1-integrin-Cy3 immunofluorescence were applied to detect the origin, growth and differentiation of stem cells in the epidermis and dermis.
RESULTS AND CONCLUSION: The epidermal stem cell population grew well on the surface of KGF-ADM and attached tightly. There were small round epidermal stem cells and polygonal terminally-differentiated cells, which presented with partly cloning growth and a tendency of merging into pieces. The results of tissue-engineered skin with KGF nanocapsules in repairing the skin defects were better than those of the blank group and the control group in 2, 4 and 6 weeks after transplantation. The transplanted skin could fuse with adjacent skin completely, but still showed some contraction. Under the microscope, they showed good epidermis with layers and normal keratose stratum, and meanwhile, there were still some β1-integrin+ cells at 8 and 10 weeks, which were epidermal stem cells or transient amplifying cells identified by immunofluorescence. These findings indicate that the tissue-engineered skin carrying KGF nanocapsules has good outcomes in repairing skin defects of nude mice, which is better than common tissue-engineered skin without KGF nanocapsules and autogeneous skin transplantation.
中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程

Key words: Tissue Engineering, Stem Cells, Skin

Li Bing-hang, Deng Li-huan, Xiang Meng-juan, Yang Bin. Repairing skin defects in nude mice using tissue-engineered skin loading keratinocyte growth factor nanocapsules[J]. Chinese Journal of Tissue Engineering Research, 2015, 19(42): 6746-6752.

Figures/Tables 5

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