Chinese Journal of Tissue Engineering Research ›› 2021, Vol. 25 ›› Issue (7): 1014-1018.doi: 10.3969/j.issn.2095-4344.2165

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Effect of peroxiredoxin 6 on proliferation and differentiation of bone marrow mesenchymal stem cells into neural lineage in vitro

Lun Zhigang, Jin Jing, Wang Tianyan, Li Aimin   

  1. Lianyungang Hospital, Xuzhou Medical University, Lianyungang 222002, Jiangsu Province, China
  • Received:2020-02-21 Revised:2020-02-28 Accepted:2020-04-11 Online:2021-03-08 Published:2020-12-08
  • Contact: Li Aimin, Chief physician, Professor, Lianyungang Hospital, Xuzhou Medical University, Lianyungang 222002, Jiangsu Province, China
  • About author:Lun Zhigang, Master candidate, Lianyungang Hospital, Xuzhou Medical University, Lianyungang 222002, Jiangsu Province, China
  • Supported by:
    the Young Talents Fund of the First People’s Hospital of Lianyungang, No. QN1702

Abstract:

BACKGROUND: It has been found that many substances can promote the differentiation of bone marrow mesenchymal stem cells into neural lineage in vitro, but the effect of peroxiredoxin 6 on bone marrow mesenchymal stem cells has not been studied.  

OBJECTIVE: To explore the effects of peroxiredoxin 6 on the proliferation of rat bone marrow mesenchymal stem cells and the ability to differentiate into neural lineages in vitro. 

METHODS: Bone marrow mesenchymal stem cells of Sprague-Dawley rats were cultured and passaged by whole bone marrow adherent culture in vitro into passage 3. The cells were assigned to five groups: PBS group, 1, 10, 100 μg/L and 1 mg/L peroxiredoxin 6 groups. CCK-8 assay was used to measure the absorbance at 450 nm of each group of cells for 9 consecutive days. Cell growth curves were drawn. Enzyme linked immunosorbent assay was utilized to measure absorbance at 450 nm in platform period. A relatively optimal concentration of peroxiredoxin 6 was selected to induce bone marrow mesenchymal stem cells to differentiate into neural lineages. PBS served as the control group. After 7 days of differentiation, immunofluorescence staining and western blot assay were used to detect the expression of neuronal marker protein NSE and glial cell marker protein GFAP.
RESULTS AND CONCLUSION: (1) 1, 10 and 100 μg/L peroxiredoxin 6 groups complied with the general growth rule of bone marrow mesenchymal stem cells, and 10 μg/L was the optimal mass concentration. (2) NSE and GFAP immunofluorescence staining showed positive reaction after peroxiredoxin 6 induction; NSE and GFAP expression levels were higher than those in the control group. (3) The results of in vitro experiments show that the proper concentration of peroxiredoxin 6 can promote the proliferation of bone marrow mesenchymal stem cells and induce differentiation into neural lineage.

Key words: stem cells, bone marrow mesenchymal stem cells, reductases, nerve, neural lineage, factor, rats, experiment

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